LOCUS       Exported     3361 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3361
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1399..1446
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3294..3310
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3311..3342
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1327
                     /translation="MKFIDTAKFTIKAGNGGNGAVSFHTALFVPNGGPNGGDGGNGGSVIFEADGGKHSLLDLKLQKQLSAQDGFKGDIKNMHGAQGKDLIVRVPVGTLIIENKTGTILADMDEDKKQVLVAKGGKGGKGNARFANSRNKAPTIFEAGEIGQFYEVKAELKVLADVGFVGLPNAGKSTLLRAISNSKPEVADYAFTTLNPQLGVSRAKDGSTFVVADLPGLIEGASLGKGLGHQFLKHIERCRVICHVLDMSGNYGQEDVIKNYELIRSELVKYNYKLDERPEIIVANKMDTDEAQLNMMEEDIKKYFKDKKVVFVSGLLKDNVDELLLKIAKELETAKYVPLWEMEQDIYEGIKVYRLEEDEEDIQIINKGNGRWEVAGDTIYKIYQKFPITTDDNLLLFNEKLKKSGVYDMLRERGVGAGDIVKVFEYELEWMD"
                     /label="obgE"
                     /note="An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control."
     primer_bind     complement(1451..1467)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1534..2122)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2313..3173)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3174..3278)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAAATTCATT GATACCGCTA AGTTCACCAT
       61 CAAAGCCGGT AACGGCGGTA ACGGCGCGGT TAGCTTCCAC ACGGCATTGT TCGTGCCGAA
      121 CGGTGGTCCT AATGGTGGTG ATGGCGGCAA CGGTGGCTCT GTGATCTTTG AGGCAGACGG
      181 TGGTAAGCAC AGCCTCCTGG ATCTGAAACT GCAAAAACAA TTAAGCGCGC AAGACGGTTT
      241 TAAGGGCGAT ATTAAAAACA TGCATGGTGC GCAGGGTAAG GACCTGATCG TTCGTGTGCC
      301 GGTTGGTACC CTGATCATCG AGAATAAAAC CGGTACGATC CTGGCGGATA TGGACGAGGA
      361 CAAGAAACAA GTGCTGGTTG CGAAGGGTGG TAAGGGTGGC AAAGGTAATG CACGTTTTGC
      421 GAATTCCCGT AATAAAGCAC CGACCATCTT TGAGGCAGGC GAAATTGGTC AGTTCTACGA
      481 AGTTAAAGCG GAACTGAAAG TCCTGGCCGA CGTGGGCTTT GTGGGCCTGC CTAACGCGGG
      541 CAAATCTACC CTGCTGCGTG CGATCTCGAA CAGCAAACCG GAGGTTGCAG ACTATGCCTT
      601 TACCACGCTT AACCCGCAGC TGGGCGTTAG CCGCGCCAAA GATGGCAGCA CCTTCGTGGT
      661 GGCGGACTTA CCGGGCCTGA TTGAAGGCGC AAGCTTGGGT AAGGGTCTGG GCCATCAGTT
      721 CCTGAAACAC ATCGAACGTT GCCGCGTTAT TTGTCACGTG CTGGACATGA GCGGTAACTA
      781 CGGTCAAGAG GACGTGATTA AAAACTATGA GCTGATCCGC AGCGAACTGG TTAAATACAA
      841 CTACAAGCTG GATGAGCGTC CGGAAATCAT TGTGGCAAAC AAGATGGATA CCGATGAGGC
      901 CCAGCTGAAT ATGATGGAGG AGGACATTAA GAAGTACTTT AAGGATAAGA AGGTTGTTTT
      961 CGTTAGCGGT TTGCTGAAAG ACAATGTCGA TGAATTGTTA CTGAAGATCG CGAAAGAGCT
     1021 GGAAACCGCC AAATATGTTC CGCTGTGGGA AATGGAGCAA GACATCTACG AGGGTATCAA
     1081 GGTTTATAGA TTGGAAGAGG ACGAGGAGGA TATTCAAATT ATTAATAAGG GTAATGGTCG
     1141 TTGGGAAGTC GCGGGTGACA CTATTTACAA AATTTATCAG AAGTTCCCGA TTACGACCGA
     1201 CGACAATCTG CTGCTGTTCA ATGAAAAGCT GAAAAAGAGC GGTGTTTATG ACATGCTGCG
     1261 CGAACGTGGT GTCGGTGCTG GTGACATTGT CAAGGTGTTC GAGTATGAAC TGGAATGGAT
     1321 GGATTGAAGA GCTTAGAGAC CCCGATAGTT CCAGCTAGCA TCGCTGAGGT GTCAATCGTC
     1381 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1441 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1501 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1561 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1621 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1681 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1741 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1801 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1861 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1921 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1981 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2041 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2101 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2161 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2221 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2281 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2341 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2401 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2461 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2521 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2581 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2641 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2701 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2761 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2821 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2881 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     2941 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3001 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3061 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3121 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3181 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3241 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3301 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3361 G
//