LOCUS       Exported     2800 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2800
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      838..885
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2733..2749
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2750..2781
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..766
                     /label="Mfl524"
                     /note=""
                     /translation="MNFYDELIIELKELVLKNDFETALLKINQELSMPYVPSDIEEQLLEFKTQIFEQISKKANSNNNLNAEYILQLLKSSDREQQAFGINFLNKINLRDKLNDVSELLTLEKIDNSIKTLILFTLKEQEINKSFDVLYSGVQQSWNPIEIDFENNFKFITEIDEVISKSIENENPGIAHDAKMLNINFYLNNLDKFNNSFLNEKAAAFILIAIEMLEEKKEMSYIKQWFNFDEDKAHEILKEIKGYEQ"
     primer_bind     complement(890..906)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(973..1561)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1752..2612)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2613..2717)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GAATTTTTAC GATGAACTGA TTATCGAGCT
       61 GAAAGAACTG GTGTTAAAGA ACGACTTCGA GACTGCTCTG CTGAAAATTA ATCAAGAGCT
      121 GAGCATGCCG TATGTGCCGA GCGACATCGA GGAGCAACTG CTGGAGTTCA AGACCCAAAT
      181 CTTCGAGCAG ATTTCTAAGA AGGCGAATTC TAACAACAAT TTGAATGCAG AATATATCCT
      241 GCAACTGTTG AAAAGCAGCG ACCGTGAACA ACAGGCTTTC GGTATTAACT TTCTGAATAA
      301 GATCAACCTC CGTGACAAAC TGAACGACGT GAGCGAACTG CTGACTCTGG AAAAGATCGA
      361 CAACAGCATT AAGACGCTGA TCCTGTTTAC GTTGAAAGAA CAGGAGATCA ACAAGTCCTT
      421 CGATGTGCTG TACTCCGGTG TGCAGCAGTC CTGGAACCCG ATCGAAATTG ATTTTGAAAA
      481 CAACTTCAAA TTCATCACCG AGATCGACGA AGTCATTTCT AAAAGCATTG AAAACGAAAA
      541 CCCGGGTATT GCGCATGACG CGAAGATGCT GAATATCAAC TTCTATTTGA ATAATCTGGA
      601 CAAGTTCAAC AATAGCTTTC TGAACGAGAA AGCAGCGGCG TTTATTCTGA TCGCCATCGA
      661 GATGCTGGAA GAGAAGAAAG AGATGAGCTA TATCAAACAG TGGTTCAATT TCGACGAAGA
      721 TAAGGCGCAC GAAATCCTGA AGGAGATTAA GGGTTATGAG CAGTGAAGAG CTTAGAGACC
      781 GCCTTCGCCT TTTCTAGGAT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      841 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
      901 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
      961 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1021 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1081 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1141 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1201 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1261 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1321 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1381 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1441 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1501 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1561 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1621 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1681 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1741 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1801 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     1861 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     1921 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     1981 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2041 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2101 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2161 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2221 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2281 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2341 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2401 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2461 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2521 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2581 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2641 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2701 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     2761 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//