LOCUS       Exported     3503 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3503
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3469
                     /translation="MKLNKETILNLITEHNQDDIKKANELVASDKYYRPTYHIAPPNGLLNDPNGLVYIDGEHYIHYQWSPLQPYHGMKHWRLVKTKDFVNYDDLGVSVIPTEEFERTGAFSGSAFKEKDGVKIYYTGNIEENGEMIEEVQIKADFIDEKIVNKRVVVEHDKNLFTPHVRDPKIFEYENNKYMIFGAQCKADMLGGLVFYKTDDMEKYTFDRILKPSLDQTYGYMWECPNLDYLEGKMLFMQSSEGWFNEENPYELNSSRNVVYTQIDKLDFENNKLNEKSILKTMDFGHDFYAPQTYWVDKKLLMIGWLGAVDVQYPTDEYSWHSMLTIPRELSWSKEILVQKPYSEFKKNVLTKTTKKAFKELSVNKAIHLEFDLSDNLDIKILNEKNEFIEIKFTDKEIILDRTNQTAKVDWNFETPRKALRKFKNQKVEIFIDSSSIEIFADNYETIFTSRFFVKNFNKIQLNKEHEMLLSDIKEMKLK"
                     /label="Mfl526"
                     /note=""
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATGCGTCA TTTTACACGG CGGTCTCAAA TGAAGCTGAA
     2041 TAAAGAAACC ATTCTGAACC TGATTACGGA ACACAATCAA GACGACATCA AAAAGGCGAA
     2101 CGAGCTGGTT GCCTCTGATA AATACTATCG CCCGACGTAC CATATCGCGC CGCCGAACGG
     2161 CCTGCTGAAT GATCCAAACG GCCTGGTTTA CATCGACGGC GAGCATTATA TCCACTATCA
     2221 ATGGAGCCCG TTGCAACCTT ACCATGGCAT GAAGCATTGG CGCCTGGTGA AAACGAAGGA
     2281 CTTCGTGAAT TACGACGATT TGGGCGTTAG CGTGATTCCG ACGGAAGAAT TCGAACGTAC
     2341 GGGCGCATTT AGCGGTAGCG CTTTCAAAGA AAAAGACGGC GTTAAAATTT ATTATACGGG
     2401 CAATATTGAG GAAAATGGCG AGATGATTGA GGAGGTTCAA ATCAAGGCCG ATTTCATCGA
     2461 TGAAAAAATT GTCAACAAGC GCGTGGTGGT CGAGCACGAT AAGAATCTGT TCACCCCGCA
     2521 CGTTCGCGAC CCGAAAATCT TCGAGTATGA AAATAACAAG TATATGATTT TTGGTGCGCA
     2581 GTGTAAAGCA GATATGCTGG GCGGCCTGGT TTTCTACAAG ACCGATGACA TGGAGAAATA
     2641 CACCTTCGAC CGCATCCTGA AGCCGTCCCT GGACCAGACG TACGGTTACA TGTGGGAATG
     2701 CCCGAATCTG GATTACCTGG AGGGTAAAAT GCTGTTCATG CAAAGCTCGG AAGGTTGGTT
     2761 TAACGAGGAG AACCCTTACG AGCTGAATAG CAGCCGTAAC GTGGTCTACA CGCAGATCGA
     2821 CAAACTGGAC TTCGAAAATA ACAAGCTGAA CGAAAAGAGC ATTCTGAAAA CTATGGACTT
     2881 TGGTCACGAT TTTTACGCGC CGCAGACCTA CTGGGTGGAC AAAAAGCTGT TGATGATCGG
     2941 TTGGCTGGGT GCGGTCGATG TTCAGTATCC TACGGATGAA TATAGCTGGC ACTCTATGCT
     3001 TACCATCCCG CGTGAGCTGA GCTGGAGCAA AGAGATTCTG GTGCAGAAGC CGTATAGCGA
     3061 ATTCAAGAAA AATGTCCTTA CCAAAACCAC GAAAAAAGCC TTCAAAGAGC TGAGCGTCAA
     3121 TAAGGCCATC CATCTGGAGT TCGACCTGAG CGACAACTTG GACATTAAAA TCCTGAACGA
     3181 GAAAAATGAG TTCATTGAGA TTAAATTTAC TGATAAGGAG ATCATTCTGG ACCGTACCAA
     3241 CCAGACCGCG AAAGTCGATT GGAACTTTGA AACCCCTCGT AAGGCGCTGC GTAAGTTTAA
     3301 GAATCAAAAA GTTGAAATCT TCATCGACAG CAGCAGCATC GAAATCTTTG CCGACAACTA
     3361 CGAAACCATT TTTACTAGCC GTTTCTTCGT TAAGAACTTC AATAAAATTC AGCTGAATAA
     3421 AGAGCACGAA ATGCTGCTGT CCGATATTAA GGAAATGAAG TTGAAGTGAA GAGCTTAGAG
     3481 ACCGCTGGGC ACTATGCTTT ACT
//