LOCUS       Exported     3596 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3596
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3562
                     /label="Mfl527"
                     /note=""
                     /translation="MKHAKINSHFYDECKKVREAVGGIKNIELINRCSTRIRIKVIDITKLNLNKLEGSKIFVKTLLKDDFVQLIVNENIDEVFLNLLKSLKISYEEIPNDFEVRVNKEGNFFKVVTDGISVIVKPLIPLLITMAIVATLSNVFNGIDFGSGTLSETGQFAKAVGEMFDILQKALNLAFSIMIPWSIFKLMKGSQAIGISIGIVLCFHGLISTNDIMSGEYGGIFKWFGDASFLESGYPWKISYVGQILPIVAMSFIAVYIERFANKFDIPVFKEMVAIPMITISTFFIGILLVGPLGLLLTYGMNEGVVWATTDNVAKYIFNPILGLALPWLVITGLIQVLVVINLQQFTTFGGTTMMPMFTQLNIAVATSILAVTIINRHNKELKRTAIPSYSLAYVSGSTEPALFGVGLKFVYPVIAASIGATVGIIITTFAGVICTNGNASLLVFLSITTKPEILDKFNIHTIAGGPYLWMAIAIVATFITTFFATIGLSKIKVFKEMNAKVLERDFSVN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGTATGT ATAGCGACCG CGGTCTCAAA TGAAGCATGC
     2041 AAAGATCAAC TCTCATTTCT ACGATGAGTG CAAGAAGGTC CGCGAAGCGG TCGGTGGCAT
     2101 CAAGAACATC GAGCTGATCA ACCGTTGCAG CACCCGTATT CGTATCAAAG TGATTGATAT
     2161 CACCAAGCTG AATCTGAATA AGTTGGAGGG CAGCAAAATT TTCGTCAAGA CCCTGTTGAA
     2221 AGACGACTTT GTGCAACTGA TCGTGAATGA AAACATTGAC GAGGTGTTTT TGAATCTGCT
     2281 GAAAAGCCTG AAGATTAGCT ACGAGGAGAT TCCGAATGAT TTTGAGGTGC GCGTGAACAA
     2341 AGAAGGCAAT TTCTTCAAAG TCGTTACGGA TGGTATCTCC GTTATCGTAA AGCCGTTGAT
     2401 TCCACTGCTG ATCACGATGG CGATTGTTGC TACCCTGAGC AACGTTTTCA ACGGTATTGA
     2461 CTTCGGTTCT GGCACCCTGA GCGAAACCGG TCAGTTCGCA AAAGCGGTCG GCGAGATGTT
     2521 TGATATTCTG CAAAAAGCGC TGAACTTGGC ATTTTCCATT ATGATCCCGT GGAGCATTTT
     2581 TAAATTGATG AAAGGCAGCC AGGCGATCGG CATCAGCATT GGCATCGTGC TGTGTTTCCA
     2641 CGGCCTGATC TCTACCAACG ATATTATGAG CGGTGAGTAC GGTGGCATTT TCAAATGGTT
     2701 CGGTGATGCG AGCTTCTTGG AGAGCGGCTA CCCGTGGAAG ATTAGCTATG TTGGCCAAAT
     2761 CCTGCCGATC GTCGCAATGA GCTTTATCGC GGTGTACATT GAGCGTTTCG CAAACAAGTT
     2821 TGATATCCCG GTGTTTAAGG AAATGGTCGC CATCCCGATG ATTACCATCA GCACCTTTTT
     2881 CATTGGTATC CTGCTGGTAG GTCCTCTGGG TTTGCTCCTG ACCTATGGCA TGAATGAGGG
     2941 TGTTGTGTGG GCGACGACCG ATAATGTTGC TAAGTATATC TTCAATCCGA TTCTGGGTTT
     3001 GGCGTTGCCG TGGCTTGTTA TCACGGGTCT GATCCAAGTC CTGGTCGTCA TTAATCTCCA
     3061 ACAATTTACG ACCTTCGGTG GCACCACGAT GATGCCAATG TTCACCCAGC TGAATATTGC
     3121 AGTGGCAACC TCTATCCTGG CCGTCACCAT CATTAACCGT CATAACAAAG AGCTGAAACG
     3181 TACCGCGATC CCGAGCTATT CTCTGGCGTA CGTGAGCGGT AGCACGGAAC CGGCCCTGTT
     3241 TGGCGTCGGC CTGAAATTCG TGTACCCGGT TATCGCTGCG TCCATCGGTG CGACGGTCGG
     3301 CATCATCATT ACGACTTTTG CCGGTGTCAT TTGCACCAAT GGCAACGCGA GCCTGTTAGT
     3361 GTTCCTGAGC ATCACGACGA AGCCGGAGAT TCTGGATAAA TTTAACATCC ACACGATCGC
     3421 TGGTGGCCCG TACCTGTGGA TGGCTATCGC AATTGTTGCG ACGTTTATCA CCACGTTTTT
     3481 CGCAACGATC GGTCTGAGCA AAATCAAGGT TTTCAAAGAG ATGAACGCGA AAGTGTTGGA
     3541 ACGTGACTTC AGCGTTAACT GAAGAGCTTA GAGACCGTGC TATATCTTCC TCCCGG
//