LOCUS       Exported     4229 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4229
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4195
                     /label="Mfl528"
                     /note="Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides."
                     /translation="MEDKKINTLSGTDESDEYVTLNARAKILAAGSDNFKLDVDAAKAYLKHHVEPRLVKFGSVKERIEYLVSNGYYEEELINKYTIEQIEEITDIAYGFHREFPSFMGAMKFYNSYGLKTFDGQKYLENFEDRAVMNALFLGNGNFESATKTLRQLMLGRFQPATPTFLNAGKKQRGEYVSCYLLRVEDNMESICRAVTTSLQLSKRGGGVALCLTNLRELGAPIKQIANQATGVIPVMKILEDSFSYANQLGQRQGAGAVYLNAHHPDVMSFLDTKRENADEKIRIKSLSLGLVIPDITFELAQKNEEMALFSPYDVEREYGKAMSDISITQEYQNLVKNPNIKKTYINARKFFLTVAELHFESGYPYILFDDTVNNNNAHAATGRIVMSNLCSEIAQVSTPSTFNDDLSFKSMGDDICCNLGSLNIAKVMESGAEFAEVIEHSINALDLVSRTSDLSSAPSIQQGNKKNHAVGLGAMNLHGFLATNFIWYNSIEAVDFTNMFFYAMAYNAFKASHKLALQYGTFDGFETSQFADGSWFNKYTKCAEDKWTPETQRVRELFAQYNVTIPTQKDWIELVELIKKDGIANSHLMAVAPTGSISYLSSCTPSLQPVVAPIEVRKEGKLGRIYVPAYNINFDNMAYYALGAYEVGPMPIIDIVAAAQQHVDQAISLTLFMTDQVTTRDLNKAYIHAFKKKCASIYYVRVRQEVLEDSENYECDACVI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGATATG TAGACACCGC CGGTCTCAAA TGGAGGACAA
     2041 GAAAATCAAC ACCCTGAGCG GCACGGATGA GAGCGACGAG TATGTTACGC TGAACGCGCG
     2101 TGCGAAAATC CTGGCAGCGG GTAGCGATAA CTTTAAGCTG GACGTAGACG CGGCTAAGGC
     2161 TTACCTGAAA CATCACGTCG AGCCGCGTCT GGTGAAATTC GGTTCCGTTA AGGAGCGCAT
     2221 CGAATACCTT GTCTCCAACG GTTACTACGA GGAAGAACTG ATCAACAAGT ACACCATCGA
     2281 ACAAATTGAA GAAATCACTG ATATCGCTTA CGGTTTCCAT CGTGAATTTC CGAGCTTCAT
     2341 GGGTGCTATG AAATTCTATA ATTCGTATGG TCTTAAGACC TTTGATGGTC AAAAATACCT
     2401 GGAAAATTTC GAAGATCGTG CAGTCATGAA CGCGCTGTTT CTGGGCAATG GCAACTTCGA
     2461 AAGCGCTACG AAAACCTTGC GCCAGCTGAT GCTGGGTCGC TTCCAACCAG CAACGCCGAC
     2521 CTTCCTGAAC GCCGGTAAAA AACAGCGTGG CGAGTATGTT AGCTGTTACC TTCTGCGTGT
     2581 GGAGGATAAT ATGGAAAGCA TCTGTCGTGC GGTTACCACC TCCCTGCAGC TGAGCAAGCG
     2641 TGGTGGTGGT GTGGCCCTGT GTCTCACGAA TCTGCGTGAA TTGGGTGCAC CTATTAAACA
     2701 AATTGCGAAC CAGGCGACCG GCGTTATCCC GGTTATGAAG ATTCTGGAAG ATAGCTTTTC
     2761 TTACGCAAAC CAACTGGGTC AACGTCAGGG TGCCGGTGCC GTCTATCTGA ATGCGCACCA
     2821 CCCAGATGTG ATGAGCTTCC TGGACACGAA ACGTGAGAAC GCCGATGAGA AAATCCGCAT
     2881 CAAAAGCCTG AGCCTGGGCC TGGTAATTCC GGATATTACC TTTGAACTGG CCCAGAAGAA
     2941 TGAAGAAATG GCCCTGTTCA GCCCGTATGA CGTGGAGCGT GAGTACGGTA AGGCAATGAG
     3001 CGATATTAGC ATTACCCAGG AATACCAAAA CCTGGTCAAG AATCCAAATA TCAAGAAAAC
     3061 CTACATTAAC GCCCGTAAGT TCTTCCTGAC CGTGGCAGAA CTGCATTTCG AGTCCGGTTA
     3121 CCCGTACATT CTGTTCGATG ATACCGTTAA CAATAACAAT GCACATGCGG CCACTGGTCG
     3181 CATCGTTATG TCCAATCTGT GCAGCGAAAT CGCCCAGGTG TCTACGCCGA GCACCTTCAA
     3241 CGATGACCTG TCCTTTAAAT CCATGGGTGA CGATATCTGC TGCAATCTGG GTTCTCTGAA
     3301 TATCGCGAAA GTCATGGAAA GCGGTGCAGA ATTTGCCGAG GTCATCGAAC ATAGCATCAA
     3361 CGCGCTGGAC CTCGTGTCTC GTACCTCCGA CCTGAGCTCC GCTCCGAGCA TCCAGCAGGG
     3421 TAATAAGAAA AATCACGCAG TTGGTCTGGG TGCCATGAAT CTGCATGGTT TTCTGGCGAC
     3481 CAACTTTATT TGGTATAATA GCATCGAAGC GGTGGATTTC ACCAACATGT TCTTCTACGC
     3541 CATGGCGTAC AACGCGTTTA AGGCGAGCCA CAAGCTGGCA CTCCAATATG GTACCTTCGA
     3601 TGGTTTTGAA ACGAGCCAGT TCGCAGACGG TAGCTGGTTT AATAAGTACA CGAAGTGCGC
     3661 CGAGGATAAA TGGACCCCGG AAACCCAACG CGTTCGTGAG CTGTTTGCGC AGTATAACGT
     3721 CACGATTCCG ACCCAAAAGG ACTGGATTGA ACTGGTGGAG CTGATCAAGA AAGATGGTAT
     3781 CGCAAACTCC CATCTGATGG CTGTGGCTCC AACCGGCAGC ATCAGCTATC TGAGCAGCTG
     3841 CACCCCGAGC CTGCAACCGG TTGTCGCGCC GATCGAAGTT CGTAAAGAAG GTAAATTAGG
     3901 CAGAATCTAC GTGCCGGCCT ACAATATTAA TTTTGATAAC ATGGCATACT ATGCGCTGGG
     3961 CGCGTATGAG GTCGGTCCGA TGCCAATTAT TGACATTGTC GCTGCGGCGC AGCAGCACGT
     4021 TGATCAAGCG ATTAGCCTGA CTCTGTTTAT GACCGATCAA GTCACCACCC GTGACCTGAA
     4081 CAAAGCGTAC ATCCATGCTT TTAAGAAGAA ATGTGCGTCT ATCTATTACG TCCGTGTGCG
     4141 CCAAGAGGTT CTGGAGGACT CTGAAAATTA TGAATGTGAT GCTTGCGTCA TTTGAAGAGC
     4201 TTAGAGACCC GCGGAAAATG GAAAACACT
//