LOCUS       Exported     3404 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3404
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..3370
                     /label="Mfl532"
                     /note="Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs."
                     /translation="MKNKTVNIIGAGLAGTEAAYQLAKRNIKVKLFEVKRITKNPVQVLDGFAELVCSNSLRSNELTNAVGTLKEEMRLLDSLIIRAAEYAQVPAGGSLAVDRVLFSEYITKEISSNENIEVIDLEVENINPNELTLIASGPLTTNKLQEQITKLIGNDDFYFYDAVAPIIEKESINMEIAYQKNRYDKGETSDYINCPMSKEEYLNFYKELINAEISIGHLPGEAELKYFEGCMPVEAMAKRGVDTLLFGPMKPRGLNKPDGTRNHAVVQLRQDDANDRLYNIVGFQTNLKWPEQKRIFSMIPGLENAKFVRYGVMHKNNFINSPKVLNKYLQLKTNENIFFAGQITGVEGYVESCVSGLITAINIANFINGKEMQVPSSKSVSGALLNYINKASITNFQPMKANWGIVDEIEFKRPNRKVTKQEIKKMKNEKISELAIDSIKDFIKNI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGGGGCC GTCAATATCA GGGTCTCAAA TGAAGAACAA
     2041 GACTGTAAAC ATTATTGGCG CCGGTCTGGC GGGTACCGAA GCCGCATACC AGCTGGCAAA
     2101 GCGTAATATT AAAGTGAAGC TCTTTGAGGT TAAGCGCATT ACCAAAAATC CGGTTCAAGT
     2161 GCTGGATGGC TTTGCAGAGC TGGTGTGCAG CAACAGCCTG CGTTCCAACG AGTTGACGAA
     2221 TGCAGTCGGC ACGTTGAAAG AAGAGATGCG TCTGCTGGAC AGCTTGATTA TTCGTGCGGC
     2281 GGAGTACGCG CAAGTCCCGG CGGGTGGTAG CCTGGCGGTT GATCGCGTGC TGTTCTCCGA
     2341 ATACATCACC AAGGAGATTA GCAGCAACGA AAACATCGAA GTCATCGATC TGGAAGTTGA
     2401 GAATATCAAC CCTAATGAGC TGACTCTGAT TGCTTCTGGT CCACTGACGA CTAACAAACT
     2461 GCAAGAACAG ATTACCAAAC TGATTGGTAA CGATGACTTC TATTTTTACG ATGCGGTCGC
     2521 CCCGATCATT GAGAAGGAGA GCATTAATAT GGAAATCGCA TACCAGAAAA ATCGCTATGA
     2581 CAAGGGTGAG ACTAGCGACT ATATCAACTG CCCGATGAGC AAGGAAGAGT ATCTGAACTT
     2641 CTACAAAGAG CTGATCAACG CAGAGATTAG CATTGGCCAC TTGCCGGGTG AGGCGGAGCT
     2701 GAAATATTTT GAAGGCTGCA TGCCGGTCGA GGCAATGGCG AAGCGTGGTG TGGATACGCT
     2761 GCTGTTTGGT CCGATGAAAC CGCGTGGTCT GAACAAGCCG GATGGCACCC GTAACCACGC
     2821 GGTGGTGCAG CTGCGCCAGG ACGATGCCAA TGATCGTTTG TACAATATTG TTGGCTTTCA
     2881 GACGAATCTG AAATGGCCGG AGCAGAAACG CATCTTTTCT ATGATTCCGG GCTTGGAGAA
     2941 CGCTAAGTTC GTGCGCTACG GCGTCATGCA TAAAAACAAC TTTATCAATA GCCCGAAAGT
     3001 CCTGAACAAA TATCTGCAAC TGAAAACCAA TGAAAACATC TTCTTTGCCG GTCAAATCAC
     3061 GGGTGTTGAA GGTTACGTCG AAAGCTGTGT CAGCGGCCTG ATCACCGCGA TCAATATCGC
     3121 GAACTTTATT AATGGCAAAG AGATGCAAGT GCCGAGCTCG AAAAGCGTCA GCGGCGCACT
     3181 GCTCAACTAT ATCAATAAAG CCAGCATCAC TAATTTCCAA CCGATGAAAG CTAATTGGGG
     3241 TATCGTTGAT GAAATTGAGT TTAAACGTCC GAACCGTAAG GTTACCAAGC AGGAGATCAA
     3301 GAAAATGAAG AACGAAAAGA TCAGCGAGCT GGCGATCGAC AGCATTAAAG ACTTTATTAA
     3361 GAACATTTGA AGAGCTTAGA GACCGCCCGG CAATTCTTAT AGCT
//