LOCUS       Exported     3971 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3971
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3937
                     /label="Mfl533"
                     /note=""
                     /translation="MSNNNLQNGKQLNKHGFDVNLEFSWKKMGVFMRQIFESAKENKLIFWAMCLFTMFDAIVATFLPVFATMMISAITKPGADTLNFLAWEVGLTEWTAWLYITLAALVLLLLSEYVVNWSIAQFSLHVEINQRQKMLIRLAQQDVDFFFDHVSGNILTRLVGDTQSLAFGIQQFFTNIIYFLTGITMSVVIMLLSGLWYVALILAVYMFLSIAIAVVIFIQSRRKLITAFDQKRDVDQDMTDRISNISLIKSSGLEEYEIERIEELNEKYNRAGDAAITWSAILTQWIQVTASLMMPMFVIIFCIMFAKESDLAAGIELLSVKMPLAQVLVSFVIGAIAMLIPTLRSATRAQNAAQRISELTDPIPTIKPNPEGPVIEKINSISFENVQFAYPKKPEKTILPKMNIVFEKGKSYAFVGETGSGKSTVAKLLLRFYMPTEGKIMVEGKYKNDESVSKQSHDLNEINLPAFLNKVGYVEQEPQILFGDFFENIRYAKFDATDEEIMRACKKANLHDFIISLKDGYNTILGQRGFLLSGGQKQRLVIARVFLKNPDFLILDEATSALDNIVEKEIQAELDKLMKGRTSVTIAHRLSTIKNVDQIIVLGANGKGIVQQGSYEELISTPGRFKKLYEAGLMN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCAAA TGAGCAATAA
     2041 TAATCTGCAG AACGGCAAAC AGCTGAACAA GCATGGTTTT GACGTTAACC TGGAGTTCAG
     2101 CTGGAAGAAG ATGGGTGTTT TCATGCGCCA AATTTTTGAG TCTGCGAAAG AAAACAAGCT
     2161 GATTTTCTGG GCTATGTGCC TGTTTACCAT GTTCGATGCA ATTGTCGCGA CGTTCCTGCC
     2221 AGTGTTCGCG ACGATGATGA TCAGCGCCAT TACGAAACCG GGCGCGGATA CCCTCAACTT
     2281 CCTGGCGTGG GAGGTGGGTC TGACCGAATG GACCGCGTGG CTGTATATTA CCCTGGCGGC
     2341 GCTGGTCCTG CTGTTGCTGA GCGAATACGT CGTGAACTGG AGCATTGCCC AGTTCAGCCT
     2401 GCACGTTGAG ATTAACCAAC GCCAAAAAAT GCTGATTCGC TTGGCGCAGC AAGATGTTGA
     2461 TTTCTTTTTC GATCATGTAT CTGGCAACAT TTTGACCCGC CTGGTGGGTG ACACGCAAAG
     2521 CCTGGCGTTC GGTATTCAAC AATTCTTCAC CAATATCATC TACTTTCTGA CGGGTATCAC
     2581 CATGTCCGTT GTCATTATGC TGCTGAGCGG CCTGTGGTAC GTGGCGCTGA TTCTGGCCGT
     2641 TTATATGTTC CTCTCTATCG CGATCGCGGT TGTTATCTTC ATTCAGAGCC GTCGTAAACT
     2701 GATTACGGCA TTTGACCAGA AACGCGACGT AGATCAAGAT ATGACGGACC GCATTAGCAA
     2761 CATTTCTCTG ATTAAAAGCT CGGGCCTGGA GGAGTATGAG ATTGAACGTA TCGAGGAACT
     2821 GAACGAGAAG TATAACCGCG CCGGCGACGC TGCGATCACT TGGAGCGCGA TCCTGACGCA
     2881 GTGGATTCAA GTTACGGCCT CGTTGATGAT GCCGATGTTC GTCATTATTT TCTGCATTAT
     2941 GTTCGCAAAG GAAAGCGACC TGGCAGCAGG CATCGAACTT CTGAGCGTTA AAATGCCGCT
     3001 GGCCCAGGTC CTGGTCAGCT TTGTTATCGG TGCAATCGCC ATGCTGATTC CGACGCTGCG
     3061 TTCAGCGACT CGTGCTCAAA ACGCAGCGCA ACGCATCTCC GAGTTGACGG ACCCGATCCC
     3121 GACCATTAAA CCGAACCCGG AAGGTCCGGT GATCGAGAAA ATCAATAGCA TCAGCTTCGA
     3181 AAATGTGCAA TTCGCGTATC CGAAAAAGCC GGAAAAGACC ATTCTGCCGA AAATGAACAT
     3241 CGTGTTCGAG AAAGGTAAAA GCTATGCATT CGTGGGTGAA ACCGGCAGCG GTAAATCCAC
     3301 GGTTGCGAAG CTGCTCCTGC GCTTCTACAT GCCGACCGAG GGCAAAATTA TGGTCGAGGG
     3361 TAAGTATAAG AACGACGAAT CCGTTAGCAA ACAAAGCCAT GATTTGAATG AAATCAATCT
     3421 GCCGGCATTT CTCAATAAAG TCGGCTATGT CGAGCAGGAA CCGCAGATCC TGTTCGGCGA
     3481 CTTCTTTGAA AACATCCGCT ACGCCAAATT CGATGCAACG GATGAGGAGA TTATGCGTGC
     3541 CTGTAAAAAA GCAAATCTGC ATGATTTCAT CATTAGCCTG AAGGACGGCT ACAACACCAT
     3601 CCTGGGTCAA CGTGGTTTTT TGCTGAGCGG TGGTCAAAAA CAACGTTTGG TGATTGCCCG
     3661 TGTTTTCCTG AAAAACCCGG ACTTCCTGAT TCTGGATGAG GCAACCTCTG CACTGGACAA
     3721 CATTGTCGAG AAGGAAATTC AGGCAGAGTT GGATAAATTG ATGAAGGGTC GTACCAGCGT
     3781 CACGATTGCC CACCGCCTGA GCACCATTAA AAACGTTGAC CAGATTATTG TTCTGGGCGC
     3841 GAATGGTAAA GGTATCGTCC AACAAGGCTC GTACGAAGAA CTGATTAGCA CGCCGGGTCG
     3901 CTTTAAAAAG TTGTATGAGG CAGGCCTGAT GAACTGAAGA GCTTAGAGAC CCAGCCTCTG
     3961 TGTTGTCTGA T
//