LOCUS       Exported     4043 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4043
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4009
                     /note=""
                     /translation="MIDREEQEMLDNEKINESSTPQVRVSRWLKKSDLNTELEPTKSKKKSKSYIALILRYMGKNKMWTFLMLVSVLILSVATAMTPKIIEQMSTAVVVERYKDAAEGTDLWERYSQALAKWWGVNFTGLLWIEMGIVIAMAIATFVSQWTAGMLGKKIEVDLRNDLNKKLVSMDMSYYSDKKIGEILTKVVSDTQIIGEQTGIIPITFLNGILTTIAAIIVMSTISGSLTIVLICVFLTLFLIFFILFIPMKPIAYKTRKIVTEVNGDVTDRINNVKLIKASGTEEYEENRFIEKHIPYFKQSSLMNYYQAIVLSLIFLIINAIETVMIIATVLIYDSNQVVAVLPGMISASSLMIGPLMSFLRVLVGLTQSNVASKRIGEILEQEPRFNNHFEDKNGVIVEKIKGNIYFKDVAFAYPEKPTELVLPKFDFTFEQGKSYAFVGTTGAGKSTISKLLLRFYDPTIGQVLINEDIDLKDVVLSSYLDKVGYVEQEPAIFLGNVYDNVRYGRFEATNKQVIAACKKAELHDLIMTWPDGYDTILGERGFMLSGGQKQRLVIARMFLKDPQLLILDEATSALDNIVEKEIQAKLNELMKGRTSVTIAHRLSTIKNVDEILVLAPKQGIVQRGTFNELKKIPGHFKDLYDAGNSKKDGKEVTNNEQQ"
                     /label="Mfl534"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGAACTA TGGCATCGAG CGGTCTCAAA TGATTGACCG
     2041 TGAGGAGCAG GAGATGCTGG ACAATGAGAA GATTAATGAG TCCAGCACTC CGCAAGTCCG
     2101 TGTCAGCCGC TGGCTGAAAA AGAGCGATCT GAACACTGAA CTGGAGCCGA CCAAGAGCAA
     2161 GAAGAAAAGC AAAAGCTATA TCGCCCTCAT CCTGCGTTAC ATGGGTAAGA ACAAGATGTG
     2221 GACGTTTTTG ATGCTGGTGA GCGTTCTGAT TCTGAGCGTT GCAACGGCAA TGACCCCGAA
     2281 GATTATCGAA CAAATGTCTA CCGCGGTTGT CGTGGAACGT TACAAAGACG CAGCGGAGGG
     2341 TACCGACTTG TGGGAGCGTT ACAGCCAAGC GTTGGCAAAG TGGTGGGGCG TTAACTTTAC
     2401 CGGTTTGTTG TGGATTGAAA TGGGTATCGT GATCGCAATG GCGATCGCGA CCTTCGTTAG
     2461 CCAGTGGACG GCGGGTATGC TGGGTAAGAA AATTGAAGTG GATCTGCGTA ACGACCTGAA
     2521 CAAGAAATTG GTCAGCATGG ATATGAGCTA TTATAGCGAT AAAAAGATCG GTGAAATTCT
     2581 GACGAAAGTT GTCAGCGATA CGCAAATTAT TGGCGAACAG ACCGGCATTA TCCCGATTAC
     2641 GTTCCTGAAT GGCATCCTGA CCACGATCGC TGCTATCATC GTGATGTCAA CGATCTCCGG
     2701 TTCCCTCACG ATTGTGCTGA TTTGCGTCTT TTTGACCTTA TTTCTGATCT TCTTCATTCT
     2761 GTTTATTCCG ATGAAACCGA TCGCATACAA AACCCGCAAA ATTGTTACCG AGGTGAATGG
     2821 TGATGTGACG GATCGCATCA ACAACGTTAA GCTGATCAAA GCGTCGGGTA CCGAGGAATA
     2881 TGAAGAAAAT CGTTTTATCG AAAAGCATAT CCCGTATTTC AAACAATCTA GCTTGATGAA
     2941 TTATTACCAA GCAATCGTCC TGTCTCTGAT TTTCCTGATC ATCAACGCGA TTGAGACTGT
     3001 GATGATTATC GCGACGGTGC TGATTTATGA CAGCAACCAA GTGGTGGCTG TTTTGCCGGG
     3061 CATGATCAGC GCGTCCTCCC TGATGATCGG CCCGCTGATG AGCTTCCTGC GTGTCCTGGT
     3121 GGGTCTGACC CAGAGCAACG TCGCGAGCAA ACGTATTGGT GAGATTCTGG AACAAGAGCC
     3181 GCGTTTCAAC AATCATTTCG AAGATAAAAA CGGCGTTATC GTGGAAAAAA TCAAAGGTAA
     3241 TATCTACTTT AAAGACGTGG CGTTTGCATA TCCGGAGAAA CCGACCGAAC TGGTTCTGCC
     3301 GAAATTTGAT TTTACCTTCG AGCAGGGTAA ATCCTATGCG TTTGTTGGTA CGACCGGCGC
     3361 CGGCAAGAGC ACCATCTCCA AACTGCTGCT GCGTTTCTAT GATCCGACCA TCGGTCAAGT
     3421 GCTGATTAAC GAGGACATCG ACCTGAAAGA TGTTGTGCTG AGCTCGTACC TGGATAAAGT
     3481 TGGCTACGTC GAGCAAGAGC CGGCGATTTT TCTGGGCAAC GTGTATGATA ATGTGCGTTA
     3541 CGGCCGCTTC GAAGCGACGA ATAAACAGGT GATCGCCGCA TGCAAGAAGG CGGAGCTGCA
     3601 CGACCTGATT ATGACCTGGC CGGATGGTTA CGACACCATT CTGGGCGAAC GCGGTTTCAT
     3661 GCTGTCCGGT GGTCAGAAGC AACGTTTAGT GATCGCGCGC ATGTTCTTGA AAGACCCACA
     3721 ACTGCTGATT CTGGACGAGG CGACCAGCGC TCTGGATAAC ATCGTTGAGA AAGAAATTCA
     3781 AGCAAAACTG AATGAACTGA TGAAGGGTCG TACTTCCGTG ACCATTGCGC ACCGCCTGAG
     3841 CACTATTAAA AATGTAGACG AAATCCTGGT CCTGGCGCCG AAACAGGGCA TTGTTCAGCG
     3901 CGGCACGTTC AACGAGCTGA AAAAGATCCC GGGCCACTTC AAGGACCTGT ATGACGCGGG
     3961 TAACAGCAAA AAAGACGGCA AAGAAGTCAC GAACAATGAA CAGCAGTGAA GAGCTTAGAG
     4021 ACCGTGTTCT ATGTCCCCAT CGT
//