LOCUS       Exported     2842 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2842
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      880..927
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2775..2791
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2792..2823
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..808
                     /label="Mfl535"
                     /note=""
                     /translation="MDNVLLYFSLKYHGDWEKIYDALDRKEKIETQDLIKVPNSIPNNFISIINPLYPNNLKQIMKPPFILYYLGNISLLQNYFQTIFINSSDNIDEYNFKVIKQVVEDLIKENRTILLSCENSQDNKLLDFIIEKQGKLIVVTKEPLQSFLNSESWAKHKNIEYYDFIIISEYEESNNFKYKSNNNKEEMNIRIINGLSKAIIFLEHSNFKKIEPLFNAVLNENKPYFAIPQNLKDTESSSNNLLKNGAKLLESATDILNQI"
     primer_bind     complement(932..948)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1015..1603)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1794..2654)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2655..2759)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGACTGCA TACCAGGTTG GGTCTCAAAT GGACAACGTG CTGCTGTACT TCAGCTTGAA
       61 GTATCATGGT GACTGGGAGA AGATTTATGA TGCGCTGGAT CGTAAAGAAA AAATCGAAAC
      121 CCAAGATCTG ATCAAAGTCC CGAATAGCAT CCCGAATAAT TTCATTTCCA TTATTAACCC
      181 GCTCTATCCG AATAATTTGA AGCAAATTAT GAAACCGCCT TTTATCTTAT ACTACCTGGG
      241 TAACATTAGC CTGCTGCAAA ATTATTTCCA GACCATCTTT ATTAATAGCT CTGACAACAT
      301 TGATGAATAT AATTTCAAGG TGATTAAACA GGTGGTCGAG GATCTGATCA AAGAAAATCG
      361 CACCATTCTG CTCTCCTGCG AGAACAGCCA GGACAATAAA CTGCTGGACT TCATCATTGA
      421 GAAACAAGGC AAGTTGATCG TTGTCACCAA AGAGCCGCTG CAATCTTTTC TGAACAGCGA
      481 AAGCTGGGCG AAGCATAAAA ATATCGAGTA CTACGATTTT ATCATTATCA GCGAGTACGA
      541 AGAGTCTAAC AACTTTAAGT ACAAGAGCAA CAATAACAAA GAGGAGATGA ACATCCGTAT
      601 TATCAATGGT CTGAGCAAAG CGATTATCTT CCTGGAACAT TCCAATTTTA AGAAAATTGA
      661 GCCGCTGTTC AACGCAGTGT TAAACGAGAA CAAACCGTAC TTCGCGATCC CGCAAAATCT
      721 GAAGGACACG GAGAGCAGCT CCAATAATTT GCTGAAGAAC GGCGCGAAGC TGCTGGAGAG
      781 CGCGACCGAT ATTCTGAATC AGATTTGAAG AGCTTAGAGA CCGTAGTGCC TGTTCGTTCT
      841 GTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      901 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      961 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1021 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1081 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1141 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1201 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1261 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1321 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1381 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1441 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1501 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1561 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1621 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1681 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1741 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     1801 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     1861 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     1921 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     1981 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2041 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2101 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2161 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2221 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2281 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2341 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2401 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2461 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2521 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2581 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2641 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2701 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     2761 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     2821 ACTTGGTCAG GTGGAGTGGG AG
//