LOCUS       Exported     2857 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2857
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      895..942
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2790..2806
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2807..2838
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..823
                     /label="Mfl545"
                     /note=""
                     /translation="MDNLFIINLMLLIVNFIVMISLLFSVLYFNKSYINYQVPRINSYNDVISSKEIERIIEQFKRIYHLADYEIIYADTENYISLFRNLNKSKKQIVISKKIFESVGYEIDYIISRLWIASKINEKNGLVRGYKWLLVTIPFLSLALMCICLLINCILFGYMSGRTSENTDKIILWIWKIPMFSVLFFIGFISMIMSYFFSLKVKEAIEYNYTDEISSLVKLTLEEYVQDFISARTYAQNIKISYLPLIKNSEFWENAKWVGPFVYM"
     primer_bind     complement(947..963)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1030..1618)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1809..2669)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2670..2774)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAGAACGAA CAGGCACTAC GGTCTCAAAT GGACAACCTG TTTATCATCA ATCTGATGCT
       61 GCTGATCGTC AATTTTATTG TTATGATCAG CCTGCTGTTC AGCGTTCTGT ATTTCAACAA
      121 GAGCTATATC AACTACCAAG TTCCGCGTAT TAATAGCTAC AATGATGTGA TCAGCTCTAA
      181 AGAAATTGAA CGTATTATTG AGCAGTTCAA ACGTATTTAT CATTTAGCTG ATTATGAGAT
      241 CATCTACGCA GATACGGAGA ACTATATTAG CCTGTTTAGA AATTTAAACA AGTCTAAGAA
      301 GCAAATCGTT ATCAGCAAAA AGATTTTTGA GAGCGTCGGC TACGAAATTG ACTACATTAT
      361 CAGCCGTCTG TGGATCGCGA GCAAGATCAA TGAGAAAAAC GGTCTGGTTC GTGGTTACAA
      421 GTGGCTGCTT GTGACCATTC CGTTTCTGAG CCTGGCCCTG ATGTGTATTT GCCTGCTCAT
      481 CAATTGTATC CTGTTTGGTT ACATGAGCGG TCGTACGAGC GAGAATACGG ATAAGATCAT
      541 TCTGTGGATT TGGAAAATCC CGATGTTTAG CGTGCTGTTC TTTATCGGTT TTATCAGCAT
      601 GATTATGAGC TACTTCTTTT CGCTGAAGGT CAAAGAAGCG ATTGAGTACA ACTATACCGA
      661 TGAGATCAGC AGCCTGGTTA AATTGACCCT GGAAGAATAC GTTCAGGACT TTATTTCTGC
      721 CCGCACCTAC GCACAAAATA TTAAGATTAG CTATCTGCCG TTGATTAAGA ACAGCGAATT
      781 CTGGGAGAAC GCGAAATGGG TTGGCCCGTT CGTCTACATG TGAAGAGCTT AGAGACCGAG
      841 GTCAATCATG CCTCTCTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
      901 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
      961 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1021 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1081 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1141 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1201 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1261 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1321 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1381 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1441 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1501 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1561 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1621 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1681 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1741 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1801 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     1861 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     1921 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     1981 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2041 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2101 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2161 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2221 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2281 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2341 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2401 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2461 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2521 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2581 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2641 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2761 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     2821 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//