LOCUS Exported 2896 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2896 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 934..981 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2829..2845 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2846..2877 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..862 /label="Mfl550" /note="Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves." /translation="MQHWDELNISNFSGPLDLLWNMVKDKKIDIFEINLSEIIDQYLKYIEGQQKLDIELASEYLVLAAQMIEMKSRILLPNDDDDSEEELMFEDLLEQINQYGQIKEVSEYFYNKQEEYLKTFSKPKTKKSFIQSIADRNDELMVNPLDIGIDEFAEIFQSILQRAQNFDEDFEYDENMLLEDAYAPIQNEIISPQVIARSIVDVMKTNKHKEWRLEEVITGYELNLINLISTFLAVLDMVRHQVAVINQKNDTLEFRFTSEALADESVLRRIEEVEEYE" primer_bind complement(986..1002) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1069..1657) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1848..2708) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2709..2813) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AATGTCCTTA GGCAGTCGTC GGTCTCAAAT GCAACACTGG GACGAGCTGA ACATCAGCAA 61 TTTTAGCGGC CCTCTGGATC TGCTGTGGAA CATGGTGAAA GATAAGAAAA TTGATATTTT 121 CGAAATTAAT CTGAGCGAAA TCATCGATCA ATACCTGAAG TACATCGAGG GTCAGCAAAA 181 ACTGGATATC GAACTGGCAT CCGAATACCT GGTGCTGGCG GCTCAAATGA TTGAAATGAA 241 AAGCCGTATT CTGCTGCCAA ATGATGATGA TGATAGCGAA GAAGAATTGA TGTTTGAGGA 301 TCTGCTGGAG CAAATCAATC AGTACGGTCA GATTAAAGAA GTTTCCGAGT ATTTTTACAA 361 TAAACAAGAG GAGTACCTCA AGACCTTTAG CAAACCAAAG ACCAAAAAGA GCTTTATTCA 421 ATCTATCGCG GATCGCAATG ACGAACTGAT GGTTAATCCA CTGGATATTG GCATCGACGA 481 GTTTGCCGAG ATTTTCCAGA GCATCCTGCA ACGTGCGCAA AACTTTGATG AGGACTTCGA 541 GTATGACGAG AATATGCTGC TGGAAGATGC GTATGCCCCA ATCCAGAATG AGATCATCAG 601 CCCACAAGTG ATCGCGCGTA GCATTGTTGA TGTAATGAAA ACGAACAAGC ATAAAGAATG 661 GCGCTTAGAG GAAGTGATTA CTGGTTATGA GCTGAATCTG ATCAACCTGA TCAGCACGTT 721 TCTGGCAGTT CTGGACATGG TTCGCCACCA GGTCGCAGTC ATTAATCAGA AGAATGATAC 781 CCTGGAGTTC CGCTTTACCA GCGAGGCGCT GGCGGACGAA TCGGTCCTGC GTCGCATTGA 841 AGAAGTCGAG GAATACGAGT GAAGAGCTTA GAGACCGGTA TATTTCTCCC CTCGGGCGCT 901 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 961 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 1021 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 1081 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1141 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1201 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 1261 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1321 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1381 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 1441 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 1501 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 1561 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 1621 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 1681 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 1741 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1801 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 1861 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 1921 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 1981 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 2041 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 2101 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2161 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 2221 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 2281 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2341 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 2401 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 2461 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 2521 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 2581 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 2641 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 2701 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 2761 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 2821 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 2881 TCAGGTGGAG TGGGAG //