LOCUS       Exported     2902 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2902
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      940..987
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2835..2851
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2852..2883
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..868
                     /label="Mfl551"
                     /note=""
                     /translation="MNELKLIDYSEKIKQLIKIVKNKRIKQSEADDILFFIANMIDEVILKDSTISHRLNINLIKNNRILDIDIKPSKKVVSTKDTHEFLYLLKTLLSLMTIKNYFFDFYIYSEIKMIVNYYINKSSKSKSYDSVNERFAINELEFHDQLVMYKYLYSIFDKLMFINVFFVDKYNLKSIDVKSNFIFTKDFDSVSMPLFKTTVRKLAFADYLKILNKSVSFHYIRKLRNNLEHSFTDPKSKYNIALETELLFVLVARTLIQMHRDLKNDDELLKLIKLNQVNK"
     primer_bind     complement(992..1008)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1075..1663)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1854..2714)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2715..2819)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GAACGAACTG AAACTGATCG ACTATAGCGA
       61 AAAGATTAAA CAGCTGATCA AGATCGTTAA GAACAAGCGT ATTAAGCAGA GCGAGGCGGA
      121 CGATATTCTG TTCTTCATTG CAAACATGAT CGATGAGGTG ATTCTGAAAG ATAGCACCAT
      181 CAGCCACCGC CTGAACATCA ATCTGATTAA AAATAACCGT ATCCTGGACA TCGATATCAA
      241 GCCGTCTAAG AAAGTGGTCA GCACTAAAGA CACCCATGAG TTCCTGTATT TATTAAAGAC
      301 CCTTCTGAGC TTGATGACCA TTAAGAATTA CTTCTTTGAT TTTTATATCT ACAGCGAAAT
      361 TAAAATGATT GTTAACTATT ACATTAACAA GTCCAGCAAA AGCAAAAGCT ATGATTCCGT
      421 TAATGAGCGT TTCGCGATTA ACGAACTGGA ATTCCATGAT CAGCTGGTGA TGTACAAGTA
      481 TCTGTACAGC ATCTTTGACA AATTAATGTT CATTAATGTG TTCTTTGTAG ACAAATACAA
      541 TCTCAAGAGC ATCGACGTTA AGAGCAATTT TATTTTTACC AAGGACTTTG ATAGCGTTAG
      601 CATGCCGCTG TTCAAGACGA CGGTCCGCAA GCTGGCGTTT GCGGACTACC TGAAAATCCT
      661 GAATAAGAGC GTTTCTTTCC ACTATATCCG CAAACTGCGT AACAATTTGG AGCACAGCTT
      721 CACGGATCCG AAAAGCAAGT ATAATATCGC GTTGGAAACC GAGCTCCTCT TCGTGCTGGT
      781 TGCGCGCACC CTGATTCAGA TGCACCGTGA TCTGAAAAAC GACGATGAAC TGCTGAAACT
      841 GATCAAGCTG AACCAAGTTA ACAAGTGAAG AGCTTAGAGA CCGCTTGACG GAGCATCTAT
      901 CTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      961 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1021 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1261 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1501 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1681 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1741 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1801 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     1861 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     1921 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     1981 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2041 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2101 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2161 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2221 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2281 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2341 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2401 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2461 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2521 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2581 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2641 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2701 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2761 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     2821 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     2881 ACTTGGTCAG GTGGAGTGGG AG
//