LOCUS       Exported     3724 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3724
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1762..1809
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3657..3673
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3674..3705
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1690
                     /label="argS"
                     /note=""
                     /translation="MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAENIKNELLQKDYYDQIEIAGPGFINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYVSANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTNDAGNQINILAVTVFVHYLWALGIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFLTEIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGALWLKTTEFGDDKDRVLVKKDGSLTYIVPDLATHNIRIQRTHADVLINIWGGDHHGYIPRMRAGLQLLGHNPDILEIEMVQMVRLIKDGKEYKMSKRKGTAVWLVDIMEMVGKDALRYMLASKSSSSHMDLDLDLVQQKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFNQVIQMSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNIIGVSALETM"
     primer_bind     complement(1814..1830)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1897..2485)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2676..3536)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3537..3641)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GGAAAACATT ATCAGCATCG TTAAGAAGGA
       61 CCTGAAAGAA ATCGCGCAGA AATTCAACAT CAGCAAAGAT CCAATTGTGG AAATTAACAA
      121 AAACAATATC GACTCTCACT TCTCCACGAC CCTGGCGCTG ATGTCGGCGA AGGAACTGAA
      181 ACAAAACCCG ATTCAATTGG CAGAAAACAT TAAGAATGAG CTGCTGCAAA AGGATTACTA
      241 TGACCAGATC GAGATCGCCG GCCCAGGTTT CATCAACATT AAGTTGAAAA CCGAGCTGCT
      301 GTCCACGACC ATCAAGAATA TCACGACGCT GAAGGAAGCG TACGGCAAGA ATAAGATTAA
      361 AAACAAAATT ATTAACATCG AATATGTTTC CGCGAACCCG ACCGGTTTTC TGCACGTTGG
      421 CCACGCGCGC AACGCGGTTA CGGGTTCCGT GCTGGAGGAA GTGCTGAAAT TCGACGGCTA
      481 CGAAGTCCAG ACCGAGTTCT ATACCAACGA TGCGGGTAAC CAGATCAACA TTCTGGCAGT
      541 CACCGTGTTC GTCCACTATC TGTGGGCACT GGGCATTGAA ACCGAGAAGC CGGCTAACAC
      601 TTACGGTGGC ACGTTCTATG ACGACCTGGC AAATATTATG ATCCAGAAGT ACGGCGACAA
      661 ATTTAAAAAT TTGACCTTTA CGGAAACCGC CATCTCTGAT GAGGAAACCC ACCAGATTTT
      721 CCGTAAGGAA GCGGTCCAGC ATTTCTTGAC CGAGATTAAG CAGCAACTGA AGGACTTCGG
      781 TGTTGTCATT GACCACTACA GCTCCGAACA AGAAATGTAC GACACGAACC AGATCGAAAA
      841 ACTGCTGAAA GAGTATAAAG AGAAAAACGC GACCTACGAG GCTGACGGTG CCCTGTGGCT
      901 GAAAACCACG GAGTTTGGTG ATGATAAAGA CAGAGTGCTG GTTAAAAAAG ATGGTAGCCT
      961 GACGTATATC GTCCCGGATT TAGCAACGCA CAATATCCGC ATCCAGCGTA CGCATGCGGA
     1021 CGTCCTGATT AATATCTGGG GCGGCGATCA TCATGGCTAC ATTCCTCGCA TGCGCGCAGG
     1081 CCTGCAGCTG TTGGGTCACA ATCCTGACAT CCTGGAGATT GAGATGGTTC AAATGGTTCG
     1141 TTTGATCAAA GATGGTAAGG AGTACAAAAT GAGCAAACGT AAAGGTACCG CGGTGTGGCT
     1201 GGTTGACATC ATGGAAATGG TTGGCAAAGA CGCGTTACGC TATATGCTGG CGAGCAAGAG
     1261 CAGCAGCAGC CATATGGATC TGGACCTGGA CCTGGTTCAA CAAAAGAACG CGACTAACCC
     1321 GGTCTATTAT GCCCAATATG CGACCGCTCG TTGCCACTCG ATTCTGAATC AAGCCGACCA
     1381 AAAGAACATC AAGGCGAACC TGAACGTGTC AAATCTGCTG AGCAACAAAA AGGAGGTAGA
     1441 GCTGCTCCTG ACGCTGGATA ATTTTAACCA GGTTATCCAG ATGAGCGCGA AAAATCGCGC
     1501 ACCGCAACTG ATCTGTGAAT ACATCCAGAC CGTGTGTAAG CAGTTCCACA GCTATTATGC
     1561 CGATACCAAA ATCTTGGACG AAAACGATGT TCCGACCAGC GAAGCACGTC TGGGTCTGGT
     1621 GCTGAGCGTT CTCCAGGTGC TGACGAACGC CTTTAACATC ATCGGTGTCT CTGCGCTGGA
     1681 AACGATGTGA AGAGCTTAGA GACCGGTATA TTTCTCCCCT CGGGCGCTGA GGTGTCAATC
     1741 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1801 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1861 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1921 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1981 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     2041 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     2101 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     2161 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2221 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2281 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2341 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2401 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2461 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2521 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2581 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2641 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2701 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2761 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2821 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2881 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2941 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     3001 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     3061 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     3121 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     3181 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3241 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3301 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     3361 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     3421 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3481 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3541 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3601 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3661 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     3721 GGAG
//