LOCUS       Exported     4124 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4124
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4090
                     /label="Mfl562"
                     /note=""
                     /translation="MQPEATNATENIVIKTSARTSVWSALVGVAISLTNTIIQILMIYWILSAYGTEFNGFVRISMSIAIIGGTCEGALGITTVILMAKPIMEKDWITANESFSTAKRKYRNKLWRGLIFVTLISILYPLEIALAPHFLQGDPITTSITGPIIKDGMDPITIHLWELGFVCFFFGFKQVVSSSIFGIYENVLEADKQNYLRRAIILFTDVIVYMLIISLLSYINFKGIHISPVLVFLPIVIYPFIRGFMIMVYVKRKYPWIKFYSDFNDHNLVRKSSKLFFSSLGINTLMNADIIILLIVLGASGLKTTSMLSIYMIVGINIRIIMLNFIVSFREFFISQILREGRIEWKTYTNYELYTYIVAAFSFVMISILTPFVATGLFGNVIAKDFDGTEASAIYQAEALKYIFSGQEFSILFGLSTSAIIIVQGQYVLVQAKGIEKRTTKWLNLIATWYVVTEAITLVLIYFFAFNSGDKPNWIPNIIRYFYIIKLIFMMIAYFFLWSFTWTHITYNSSLKNVIPNFIFLLVPVISTSLFIKYGLSQFALIKINIATTEAGTISVIVDSISIPTIVSVLAITGITSLPICIILPLIFRPKIGLNIIVNLPIVKQIIQKNSSKLKIARFIDAGINAEEESNQNDQIMKSLYDQIEENNEIINDENYFEKRYKYKNTPKVYTLKPTETTVAKVKTKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATAGGAAC CTCTTACGCG GGGTCTCAAA TGCAGCCGGA
     2041 AGCCACCAAT GCGACCGAAA ACATCGTCAT TAAGACCAGC GCGCGCACCA GCGTTTGGAG
     2101 CGCGCTGGTT GGCGTGGCGA TCAGCCTGAC CAATACGATC ATCCAAATTC TGATGATCTA
     2161 CTGGATTCTG AGCGCGTATG GTACGGAGTT TAACGGTTTC GTGCGTATTA GCATGAGCAT
     2221 TGCAATTATT GGTGGCACGT GCGAAGGTGC GCTGGGTATC ACCACTGTCA TTCTGATGGC
     2281 GAAGCCGATC ATGGAGAAAG ATTGGATTAC GGCAAACGAG AGCTTTTCGA CCGCAAAACG
     2341 TAAGTATCGC AATAAATTAT GGCGTGGTCT GATCTTCGTG ACCCTGATCT CCATCCTGTA
     2401 CCCGCTGGAA ATCGCGCTGG CTCCGCACTT TCTGCAAGGT GATCCGATCA CCACTTCTAT
     2461 TACTGGCCCG ATCATTAAGG ACGGCATGGA TCCGATCACT ATTCATCTGT GGGAGCTGGG
     2521 CTTTGTGTGC TTCTTCTTTG GTTTCAAACA GGTTGTCTCT AGCTCCATTT TCGGCATTTA
     2581 CGAGAACGTG CTGGAGGCGG ATAAGCAAAA CTATCTGCGT CGCGCCATTA TTCTGTTTAC
     2641 GGATGTTATT GTGTATATGC TGATTATCAG CCTGCTGAGC TATATTAATT TCAAAGGTAT
     2701 CCATATTAGC CCGGTATTGG TTTTTCTGCC GATCGTGATC TACCCGTTTA TTCGTGGTTT
     2761 CATGATCATG GTCTATGTCA AACGCAAATA TCCGTGGATT AAATTCTATA GCGATTTCAA
     2821 CGATCACAAT CTGGTCCGCA AGAGCAGCAA GCTGTTTTTC AGCTCCCTTG GCATCAACAC
     2881 GTTGATGAAT GCGGACATCA TTATCTTGCT GATCGTGCTG GGTGCGAGCG GCCTCAAGAC
     2941 GACCTCTATG CTGAGCATTT ACATGATTGT GGGTATTAAC ATTCGCATCA TTATGCTGAA
     3001 TTTTATTGTT AGCTTCCGTG AGTTTTTCAT CAGCCAGATC CTGCGCGAAG GCCGCATTGA
     3061 ATGGAAAACC TACACGAACT ATGAGCTGTA CACCTACATT GTTGCGGCGT TTTCTTTTGT
     3121 GATGATTTCC ATTCTGACCC CGTTCGTCGC CACGGGCCTG TTCGGCAATG TTATCGCGAA
     3181 AGACTTTGAC GGTACGGAGG CCAGCGCAAT TTATCAAGCA GAGGCGCTGA AATACATCTT
     3241 CAGCGGTCAG GAGTTCAGCA TTCTGTTTGG TCTGTCGACG TCGGCAATTA TCATCGTCCA
     3301 GGGCCAGTAC GTCCTGGTTC AGGCGAAAGG TATTGAGAAA CGCACTACTA AGTGGCTGAA
     3361 TTTGATCGCA ACTTGGTACG TGGTTACCGA GGCGATCACG CTGGTTTTAA TTTACTTCTT
     3421 CGCCTTCAAC AGCGGCGATA AACCGAACTG GATCCCGAAT ATTATTCGTT ACTTTTACAT
     3481 CATTAAACTG ATTTTCATGA TGATTGCCTA CTTCTTTCTG TGGTCGTTTA CGTGGACCCA
     3541 TATCACCTAC AACAGCAGCC TGAAAAATGT GATCCCGAAT TTTATCTTTC TGCTGGTCCC
     3601 TGTGATCTCT ACGTCTTTGT TCATCAAATA CGGCCTGAGC CAGTTCGCAT TGATCAAGAT
     3661 TAATATTGCC ACCACGGAGG CTGGTACGAT CAGCGTTATC GTGGATAGCA TCAGCATCCC
     3721 GACGATCGTT AGCGTGCTGG CGATCACCGG CATCACCAGC CTCCCGATCT GCATCATTCT
     3781 TCCGCTGATC TTTCGTCCGA AAATCGGTCT GAATATCATC GTTAATCTGC CGATTGTCAA
     3841 GCAGATTATC CAGAAAAACT CTAGCAAACT GAAGATTGCT CGTTTTATCG ACGCCGGTAT
     3901 CAATGCGGAG GAAGAGTCTA ACCAGAATGA TCAAATCATG AAAAGCCTGT ACGATCAGAT
     3961 CGAAGAAAAT AACGAAATTA TTAATGATGA AAATTACTTC GAAAAACGTT ACAAGTACAA
     4021 GAATACCCCG AAGGTGTATA CCCTGAAACC GACGGAAACG ACCGTTGCAA AGGTTAAGAC
     4081 CAAAAAGTGA AGAGCTTAGA GACCGTGTAG TGTTCCCCAT ACGT
//