LOCUS       Exported     3830 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3830
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3796
                     /label="Mfl570"
                     /note="An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay."
                     /translation="MEKEKITSTNQIIEKEDFEPFVFKQKEVQRKYIKTESPTKVFALGGLEEIGKNTYCIEHENEIIMIDAGVKFPDDSMLGINAVIPDYSYLKENESKIKALFITHGHEDHIGGIHYLVKQVNIPVIYAPELAAALIRDRLKEHKLTDKTIVKEYVADDIWATKNLRVSYAALNHSIPDAFGILVETPNGNIFSTGDYKFDWSPLGHFAELSKLASMGDKGIELLMSDSTNAEVEGYTPGEKGIIENIDKLFLKAKGRIFITTFASNVHRIQQIVELANKYDKKVVILGRSIERIIKIIRQMGHLKINDKMFIKANDIDKHPANKIMIISTGSQGEPMAALSRIATNRHQSISIIPGDTVIFSSSPIPGNRADVEILINRLTRIGANVIESSPSNRIHTSGHASQEEQKLLFTLLRPNYFMPMHGEFRMLKKHIETAESVNLQKGHGFVMANGDQLELLQGNAQIGKRVDADAIYVDGKDMTAHASNIIRERDILSKDGLISVVVSIDSQTNKLLCAPRIISRGSFYVKDSGNVISEAITIVTEAINEVLNSNKPTFGAIKKAIKQSLSPYIFKTKRRNPLIIPVILNKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCAAA TGGAGAAAGA
     2041 AAAAATCACC TCCACGAACC AGATTATCGA GAAAGAGGAT TTTGAACCGT TCGTGTTCAA
     2101 GCAAAAAGAG GTGCAGCGTA AGTATATCAA AACCGAGAGC CCGACTAAGG TTTTCGCGCT
     2161 CGGTGGTCTG GAAGAAATTG GCAAGAACAC GTACTGCATT GAGCACGAAA ATGAGATCAT
     2221 TATGATTGAT GCGGGTGTGA AATTCCCAGA CGATAGCATG CTGGGTATCA ATGCGGTCAT
     2281 CCCGGACTAC AGCTACCTGA AGGAAAACGA GAGCAAAATT AAGGCACTGT TCATCACCCA
     2341 CGGTCACGAG GATCACATTG GTGGTATCCA CTATCTGGTG AAACAAGTGA ATATCCCGGT
     2401 GATCTACGCA CCGGAACTCG CAGCCGCGCT TATTCGTGAT CGTCTGAAGG AGCACAAATT
     2461 AACCGACAAA ACTATCGTGA AAGAGTATGT GGCGGACGAC ATTTGGGCCA CCAAAAATCT
     2521 GCGTGTCAGC TACGCAGCGC TGAATCATTC TATCCCGGAT GCTTTTGGCA TTCTGGTGGA
     2581 AACGCCGAAT GGCAATATCT TTTCCACCGG TGACTACAAG TTCGACTGGA GCCCGCTGGG
     2641 CCATTTCGCG GAGTTGTCGA AGCTCGCGAG CATGGGCGAT AAGGGTATTG AACTGCTGAT
     2701 GAGCGATAGC ACGAACGCGG AGGTTGAGGG TTACACCCCG GGCGAGAAGG GCATCATCGA
     2761 GAATATCGAC AAGCTGTTCC TTAAGGCGAA GGGTCGTATT TTCATCACCA CGTTCGCGAG
     2821 CAATGTCCAC CGCATTCAAC AGATCGTTGA GCTGGCGAAC AAATACGACA AAAAAGTTGT
     2881 TATTCTGGGC CGTAGCATTG AGCGTATTAT TAAAATTATC AGACAAATGG GTCATCTCAA
     2941 GATTAACGAT AAAATGTTCA TCAAAGCAAA TGACATCGAT AAGCACCCGG CCAACAAAAT
     3001 CATGATTATT AGCACGGGTT CCCAAGGTGA GCCGATGGCG GCTCTGAGCC GTATTGCCAC
     3061 CAATCGTCAC CAGAGCATTT CCATTATTCC AGGTGACACC GTGATCTTCT CCTCCAGCCC
     3121 GATCCCGGGT AACCGCGCTG ACGTCGAGAT CCTGATTAAC CGTTTGACGC GTATTGGTGC
     3181 GAATGTGATC GAGTCCAGCC CGAGCAACCG TATTCATACC AGCGGTCACG CGAGCCAAGA
     3241 GGAGCAGAAG TTACTGTTTA CCCTGCTGCG TCCGAATTAT TTTATGCCGA TGCACGGTGA
     3301 ATTCCGTATG CTGAAAAAGC ATATCGAAAC GGCGGAAAGC GTGAATTTAC AAAAGGGTCA
     3361 CGGTTTTGTG ATGGCGAATG GCGACCAGCT GGAATTGCTG CAGGGTAACG CGCAGATTGG
     3421 TAAGCGCGTC GACGCTGATG CGATCTATGT TGATGGTAAA GACATGACCG CGCACGCGAG
     3481 CAACATCATC CGTGAACGTG ATATCCTTAG CAAGGATGGT CTGATTAGCG TTGTCGTCAG
     3541 CATCGACTCC CAGACCAACA AACTGCTGTG CGCTCCGCGT ATCATCAGCC GCGGTTCGTT
     3601 CTACGTGAAA GATTCCGGTA ACGTTATTAG CGAGGCCATC ACGATCGTTA CGGAGGCCAT
     3661 CAATGAGGTG TTAAATAGCA ACAAACCGAC CTTCGGTGCC ATCAAAAAGG CGATTAAACA
     3721 AAGCCTGAGC CCGTATATCT TTAAGACCAA GCGTCGTAAC CCGCTGATTA TCCCGGTAAT
     3781 TCTGAACAAA AAGTGAAGAG CTTAGAGACC GAGGTCAATC ATGCCTCTCT
//