LOCUS       Exported     4679 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4679
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4645
                     /label="Mfl571"
                     /note=""
                     /translation="MTKRLSNSILNQKAFKIKDNYSKSPKKIFFWSITLFTLFIVILSFFTLDSKWLEFFRDMPSLFERIGEMFKWDWTDFSTINGTGHSFLYNAFVSIWDTIVMAFAGTVIGVVIAIPVAILASSNIVKNKSVNFIARLILSIFRTIPSFVYALVLVNYFGATTFTVTLSLTMFTFSISGKTLYERIEQINIKIFTASQSTGANKSVSFRAAVWPQVSHHVLSIMFYSLETNIRYVSIIAGVTRMGIGQMINNAVDYNEWNRVGFLLTLLVAVILFLELSIWLIRNYIIEDKDFRIDGKEQRKFDKKINKIKSQKDINFYIKNVLCVDIDKKINDSKSQENTKKLVEEKKELINNFKSDLSTKIENDIEAYKKLKKSNPNSFDLYAKDFETGLRYRIDKVNKVKFKFKVNEIKNAKIEEIKNERADAHKNFIENLSVEKVLRSEPKNYIKRIVLYAIILGFFIYTLTLLEFKLSSKELIEATNKNLLEILKINWSSLFISKANGGNSNAPYSVMYLLYETLSIAVVGTFIGAVIAYVLGMLSSEKIVNKYVARIFVALTSMMRAIPSYIYALIFVIVVGMGPFTGVLALIMGTIGMLTKYNRELFDDINQKIIFQLEATGVNWFTKLRYGIMSQTSTAAMSNIIYRFDINFKEVAMLGAVGAGNMGYLLNSYFSDQYFNEFGALLFGIILFTLLIEFISASIRNKLSFGTNLNWISSIINFVNQRYFATFKSNEKQLNIDSKLSYEESMSLYAYTNQTILNNAIAMKKEEKLSFKDAWNKAYIDFYDIRKKYDSSITDNNIVKLEELKFKNNKKDFASKRKAWVVQVRQESKLEIIKFRKSLKTTTDLKTRKDLKNSIKYSKNIKKLKITNINY"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGACGAAACG
     2041 TCTGAGCAAC AGCATTCTGA ACCAAAAAGC GTTTAAGATT AAAGATAACT ATTCCAAGAG
     2101 CCCTAAGAAA ATCTTTTTCT GGTCCATCAC TCTGTTTACC CTGTTCATCG TTATCCTGTC
     2161 ATTCTTCACT CTGGACAGCA AATGGCTGGA ATTCTTCCGC GACATGCCGT CGCTGTTCGA
     2221 ACGCATTGGC GAGATGTTCA AATGGGACTG GACTGATTTT AGCACCATCA ACGGTACCGG
     2281 CCACAGCTTT CTGTACAACG CGTTCGTGAG CATTTGGGAC ACGATCGTCA TGGCATTCGC
     2341 GGGTACCGTC ATTGGCGTGG TGATCGCGAT TCCGGTGGCG ATTCTGGCAA GCAGCAACAT
     2401 TGTCAAAAAT AAGAGCGTGA ACTTTATCGC GCGCCTGATT CTGAGCATTT TCCGCACTAT
     2461 CCCGAGCTTC GTGTACGCAC TTGTTCTGGT GAACTATTTT GGTGCGACGA CTTTTACGGT
     2521 TACCCTGAGC CTGACCATGT TCACCTTTAG CATCAGCGGC AAAACCCTGT ACGAACGTAT
     2581 TGAGCAGATC AATATTAAGA TCTTTACCGC AAGCCAGAGC ACCGGTGCAA ACAAGAGCGT
     2641 CAGCTTTCGT GCGGCAGTTT GGCCACAGGT GAGCCACCAT GTTCTGTCCA TCATGTTTTA
     2701 CAGCCTGGAA ACCAACATTC GCTATGTCTC CATTATCGCG GGTGTCACCC GCATGGGTAT
     2761 TGGCCAGATG ATTAACAATG CAGTGGACTA CAATGAGTGG AATCGTGTTG GTTTCCTGCT
     2821 GACGCTGTTG GTGGCAGTAA TCCTGTTCCT GGAACTGAGC ATTTGGCTGA TTCGTAATTA
     2881 CATTATTGAG GACAAGGATT TTCGCATCGA TGGCAAGGAA CAACGCAAAT TTGACAAGAA
     2941 GATTAATAAG ATCAAGAGCC AGAAAGATAT CAATTTTTAC ATTAAAAACG TGCTGTGTGT
     3001 GGACATCGAC AAGAAAATCA ACGACAGCAA ATCCCAAGAG AACACTAAAA AGCTGGTCGA
     3061 GGAGAAGAAG GAACTGATTA ATAATTTCAA AAGCGACCTG TCTACCAAAA TTGAAAACGA
     3121 TATCGAAGCC TATAAGAAAC TCAAGAAAAG CAACCCGAAT TCTTTTGATC TGTACGCAAA
     3181 GGACTTTGAA ACCGGCCTGC GTTATCGCAT TGACAAGGTC AACAAGGTTA AGTTCAAATT
     3241 CAAAGTGAAT GAGATTAAGA ATGCGAAAAT CGAGGAGATC AAGAACGAGC GCGCAGATGC
     3301 CCACAAGAAC TTTATCGAAA ACTTGTCCGT CGAGAAAGTT CTGCGTAGCG AACCTAAGAA
     3361 CTATATTAAA CGTATCGTCC TGTACGCGAT CATCCTGGGT TTCTTTATCT ATACCCTGAC
     3421 GCTGCTCGAG TTCAAGCTGA GCTCCAAAGA GCTGATCGAA GCGACGAACA AGAACTTGCT
     3481 GGAAATCCTG AAAATTAATT GGTCTAGCTT ATTTATCAGC AAAGCGAACG GTGGTAATTC
     3541 TAATGCACCA TACAGCGTTA TGTATCTGCT GTACGAAACG TTGTCTATCG CTGTCGTCGG
     3601 CACCTTTATT GGCGCGGTCA TTGCTTACGT TTTGGGCATG CTGTCCAGCG AGAAGATTGT
     3661 TAATAAGTAT GTCGCCCGTA TCTTCGTGGC GCTGACGAGC ATGATGCGCG CTATTCCGAG
     3721 CTATATCTAT GCATTGATCT TCGTCATCGT TGTAGGTATG GGTCCGTTCA CCGGTGTGTT
     3781 GGCCCTGATC ATGGGTACGA TTGGTATGCT GACCAAATAT AATCGCGAAC TGTTTGATGA
     3841 CATTAACCAA AAAATTATTT TTCAGCTCGA GGCCACGGGT GTGAATTGGT TCACCAAGTT
     3901 ACGTTATGGT ATTATGAGCC AGACCTCCAC CGCAGCAATG TCCAATATTA TTTATCGTTT
     3961 TGATATCAAT TTCAAGGAAG TCGCCATGCT GGGTGCGGTG GGCGCTGGTA ACATGGGTTA
     4021 TCTGTTGAAT TCCTACTTTT CCGATCAGTA CTTTAACGAG TTTGGCGCAC TGCTGTTTGG
     4081 CATTATTCTG TTCACCCTGC TGATCGAGTT CATCTCGGCT AGCATTCGCA ATAAATTGAG
     4141 CTTCGGTACG AATCTTAACT GGATCAGCAG CATCATTAAC TTCGTGAACC AGCGTTATTT
     4201 CGCCACCTTT AAGTCTAATG AAAAGCAACT GAATATCGAT AGCAAACTGA GCTACGAGGA
     4261 AAGCATGAGC CTGTACGCTT ATACGAACCA GACGATTTTA AATAATGCAA TCGCCATGAA
     4321 GAAAGAGGAA AAATTAAGCT TTAAAGACGC GTGGAACAAG GCGTATATTG ATTTTTATGA
     4381 TATCCGTAAG AAGTACGATT CCTCCATCAC CGACAATAAC ATCGTTAAAT TGGAGGAACT
     4441 GAAGTTTAAG AATAACAAAA AGGATTTCGC TTCTAAGCGC AAAGCCTGGG TTGTCCAAGT
     4501 ACGTCAAGAG AGCAAACTGG AAATCATCAA ATTTCGCAAG AGCCTCAAGA CGACGACGGA
     4561 TTTAAAGACC AGAAAAGACC TGAAGAACAG CATTAAGTAT AGCAAGAATA TCAAGAAACT
     4621 GAAGATTACG AACATCAATT ACTGAAGAGC TTAGAGACCG TAGTGCCTGT TCGTTCTGT
//