LOCUS Exported 2749 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2749 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 787..834 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2682..2698 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2699..2730 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..715 /label="Mfl576" /note="Probably transports riboflavin." /translation="MDKKYKLWNYKYDFSEINLKNWKEVLKDTFKLNTRKIALLSMLFAIEILMTIISKVIMGLAIPMIVGVYTIEISFFVILIIYLCSNYIYASILSITAIWFRLLLGSEPVGLLSMMISDTAFLTIFAVLFFILKKFIFLKFKFKNQIKILIALICFAGLISMIGSGFISMLCNDKFIFEMYYLSDDGSGYWKMLLWVGFGVTLAKYSINILLFASTLKVLLILIKQSRV" primer_bind complement(839..855) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(922..1510) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1701..2561) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2562..2666) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GGACAAAAAG TACAAGCTGT GGAATTACAA 61 GTATGATTTC AGCGAAATTA ACCTGAAAAA CTGGAAGGAA GTTCTGAAGG ACACCTTTAA 121 GCTGAATACC CGCAAGATTG CGCTGCTGAG CATGCTGTTC GCGATTGAGA TCCTGATGAC 181 GATCATTTCC AAAGTGATCA TGGGTCTGGC AATCCCGATG ATTGTCGGTG TCTATACCAT 241 CGAAATTAGC TTCTTCGTTA TTCTCATCAT TTACCTGTGT AGCAATTACA TCTACGCCAG 301 CATTCTGAGC ATCACGGCAA TTTGGTTTCG CCTGTTGCTG GGTAGCGAGC CTGTAGGTCT 361 GTTGAGCATG ATGATCAGCG ACACTGCGTT TCTGACCATC TTTGCCGTTC TGTTCTTCAT 421 TCTGAAGAAA TTCATTTTCC TGAAATTCAA GTTTAAGAAC CAAATCAAAA TTCTGATTGC 481 TCTGATTTGC TTTGCGGGTC TGATCAGCAT GATCGGTAGC GGCTTCATCT CTATGCTGTG 541 TAATGACAAG TTTATCTTCG AAATGTACTA TTTGAGCGAC GACGGTAGCG GTTACTGGAA 601 GATGCTGCTG TGGGTTGGCT TTGGTGTGAC CTTGGCGAAA TACTCCATCA ATATTCTGTT 661 GTTCGCATCT ACCTTGAAGG TGCTCCTGAT TCTGATCAAA CAGAGCCGCG TGTGAAGAGC 721 TTAGAGACCG CGGGTTAGAT ACTGCAACTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG 781 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 841 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 901 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 961 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1021 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1081 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1141 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1201 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1261 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1321 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1381 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1441 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1501 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT 1561 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 1621 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 1681 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 1741 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 1801 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT 1861 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 1921 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 1981 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 2041 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 2101 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 2161 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 2221 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 2281 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 2341 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 2401 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 2461 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 2521 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 2581 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 2641 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG 2701 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG //