LOCUS       Exported     4751 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4751
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4717
                     /note="In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity."
                     /translation="MKKILLVDGNSLLFRAYYASAYAGPVLKTTTGIPTNAIFSFANMLTSLISDRNYYDVKVAFDKSKKTFRHDKLENYKAGRSATPEDLIPQFQIVREFLDSSNISWWEKENYEADDLIGTMSKIIENNFSEFEVEILTSDKDMFQLISDKTKILLPKTGTSNLELFGEAELLEKWEVAPKNVVDLKGLMGDPSDNLKGVEGVGEKTAIKLLKEFQTIEGIYENIDSISGKLQEKLINGKDSAILCKEIATINCEVEIENLVFEPINLNLNGLINFLEKYEMNSLVKRLSTRVGNINGKDEQINKEEIKFNQLKKWNNEFSCETNFLYIESIDENYHKGQILGLAICNEKGFFYLNDIINDLELEQFFLNDNFKKNTYDIKKTVTLLRNNNIKFSFSSFTYDAMIASYVLNPNVTSRIQNLANIVETELFVEEDELFYGKGVKQNKEIDTASKAKFIVGKLELLYSTHKPIINLLKENNQFNLYEKIELPLVEVLFNMEQKGILIDKVELDKQTARTLFLIEELEEKMKVILKDQISENFNFGSPKQIKELLFDQIGLPSNKKQSTDKEALEKIIHLHPVINLLLEHRKLNKLYSTYLRGFEKFIFDDNKVHTIFNQTLTNTGRLSSSEPNIQNISVKDELQREARKIFISNNDTKFFSFDYSQIELRVLAQLGQEDTLLNIFKNDRDVHAEAARKIFKLEENEEITSDQRRIAKVFNFGIIYGLSDFGLSNDLKISIPEAKRFIDDYYLSFPKLMTYKNSLINQATVNGYAETYANRRRIVNELASSNFMVKNFGNRIAVNMPIQGTAADILKVAMNNIHKEFRENNLKSFMTAQIHDEIIFEIFESEESLAVKIVEECMQNAFIDLSKIVQKEEQPIEVKLEVNKSKGNNWFELK"
                     /label="Mfl582"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGACTGC ATACCAGGTT GGGTCTCAAA TGAAGAAGAT
     2041 TCTGCTTGTC GACGGTAACT CCCTGCTGTT CCGTGCATAT TATGCGTCTG CGTATGCGGG
     2101 TCCGGTTTTA AAGACGACCA CTGGCATCCC AACCAATGCG ATCTTCTCCT TTGCGAACAT
     2161 GCTGACCTCT CTGATCAGCG ACCGTAATTA TTACGATGTG AAAGTTGCCT TCGACAAGTC
     2221 CAAGAAAACG TTTCGTCATG ACAAGCTGGA AAACTACAAA GCGGGTAGAA GCGCGACCCC
     2281 GGAGGATCTG ATTCCGCAGT TTCAAATTGT GCGTGAGTTC TTGGATAGCA GCAATATTAG
     2341 CTGGTGGGAA AAGGAAAACT ATGAGGCGGA TGACCTGATT GGCACCATGA GCAAGATCAT
     2401 TGAAAACAAT TTTAGCGAAT TTGAGGTGGA GATTCTGACG TCTGATAAAG ATATGTTCCA
     2461 ACTGATTAGC GACAAGACTA AGATTCTGCT GCCGAAAACG GGCACCAGCA ACCTTGAACT
     2521 GTTCGGCGAG GCGGAACTGC TGGAAAAATG GGAGGTTGCC CCGAAAAACG TGGTCGACCT
     2581 GAAAGGCCTG ATGGGCGATC CTAGCGACAA CTTGAAGGGT GTAGAGGGCG TGGGTGAAAA
     2641 GACCGCAATT AAACTGCTGA AAGAGTTCCA GACGATCGAG GGTATCTACG AGAATATTGA
     2701 CAGCATCAGC GGCAAATTAC AGGAGAAACT GATCAATGGT AAGGATAGCG CAATCCTGTG
     2761 CAAAGAGATC GCGACCATCA ACTGCGAGGT CGAGATCGAG AATCTGGTTT TCGAGCCGAT
     2821 CAATCTGAAT CTGAATGGTC TGATCAATTT CCTGGAGAAA TACGAAATGA ATAGCTTGGT
     2881 TAAACGTCTG AGCACCCGTG TGGGTAATAT TAACGGCAAG GACGAGCAAA TTAACAAAGA
     2941 GGAGATTAAG TTCAACCAAT TGAAAAAGTG GAACAACGAG TTTAGCTGCG AAACCAATTT
     3001 CCTGTACATC GAAAGCATTG ATGAAAACTA CCATAAGGGT CAGATCCTGG GTCTGGCAAT
     3061 TTGTAATGAG AAAGGCTTCT TCTACCTGAA TGACATTATT AATGATCTGG AGCTGGAGCA
     3121 GTTTTTCCTG AACGACAACT TTAAGAAAAA TACCTACGAC ATCAAGAAAA CCGTCACCCT
     3181 GCTGCGTAAC AATAACATCA AGTTCAGCTT CAGCAGCTTT ACGTATGACG CTATGATTGC
     3241 CAGCTACGTC CTGAACCCAA ACGTTACTAG CCGCATCCAG AACCTGGCGA ACATTGTTGA
     3301 AACTGAACTG TTCGTGGAAG AGGACGAGCT GTTTTATGGC AAGGGCGTCA AACAAAACAA
     3361 GGAAATTGAT ACGGCTAGCA AGGCGAAGTT CATCGTCGGT AAGCTGGAGC TGTTATACAG
     3421 CACTCATAAA CCGATTATCA ATCTGCTGAA GGAGAATAAC CAGTTCAATC TGTATGAGAA
     3481 GATCGAACTG CCGCTGGTTG AAGTCTTATT CAATATGGAA CAAAAGGGTA TCCTGATTGA
     3541 TAAGGTTGAG CTGGACAAGC AGACCGCGCG TACCTTGTTT CTGATCGAGG AATTGGAGGA
     3601 AAAGATGAAG GTTATTCTGA AGGATCAGAT TAGCGAGAAC TTTAACTTTG GTAGCCCGAA
     3661 ACAGATTAAG GAACTTCTGT TCGACCAAAT CGGCCTGCCG AGCAACAAGA AGCAGAGCAC
     3721 CGACAAGGAA GCACTGGAAA AGATTATCCA TCTGCACCCG GTTATTAATC TGCTGCTGGA
     3781 GCATCGTAAG CTGAACAAGC TGTACAGCAC CTATCTGCGT GGCTTTGAAA AGTTTATTTT
     3841 CGACGATAAT AAAGTGCACA CGATTTTTAA CCAGACCCTT ACCAATACCG GCCGTCTGTC
     3901 TAGCTCTGAA CCGAATATTC AAAACATCTC CGTGAAAGAC GAGCTGCAAC GTGAGGCGCG
     3961 TAAGATCTTC ATTAGCAACA ATGACACGAA ATTTTTCTCT TTTGATTATA GCCAAATCGA
     4021 GCTGCGTGTG TTGGCGCAGC TGGGCCAGGA GGATACTCTG CTGAATATTT TCAAGAACGA
     4081 CCGTGATGTG CACGCGGAGG CGGCGCGCAA GATCTTTAAG TTGGAGGAGA ACGAAGAAAT
     4141 CACCAGCGAC CAACGTCGTA TCGCGAAGGT TTTCAACTTC GGCATCATCT ATGGTCTGTC
     4201 TGATTTCGGC CTGAGCAATG ACCTGAAAAT CAGCATTCCG GAGGCTAAAC GCTTCATCGA
     4261 TGATTACTAT CTGTCGTTTC CGAAGCTGAT GACCTATAAG AATAGCTTGA TTAACCAAGC
     4321 TACGGTGAAT GGCTATGCGG AAACGTACGC AAACCGCCGT CGTATCGTGA ACGAGCTGGC
     4381 AAGCAGCAAC TTCATGGTTA AAAACTTCGG TAACCGTATC GCGGTGAATA TGCCGATTCA
     4441 GGGCACCGCG GCAGATATCC TGAAGGTTGC TATGAACAAC ATCCATAAGG AATTCCGCGA
     4501 AAACAACCTT AAAAGCTTCA TGACGGCGCA AATCCATGAT GAGATCATTT TTGAAATTTT
     4561 CGAGAGCGAA GAGAGCCTGG CAGTTAAAAT CGTTGAGGAG TGTATGCAAA ACGCTTTTAT
     4621 CGACTTGTCA AAAATTGTTC AGAAGGAGGA GCAACCGATC GAGGTGAAGC TGGAGGTTAA
     4681 CAAAAGCAAA GGCAATAACT GGTTCGAGTT GAAGTGAAGA GCTTAGAGAC CCGGTGCTTT
     4741 TGCGTGATAT T
//