LOCUS       Exported     5042 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5042
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..5008
                     /label="Mfl583"
                     /note=""
                     /translation="MSNFIPLINVRSVYNFQESLIKINDYISFAKNEGFKHLFYCENKTMYGVAEFFQKALKENIKPIIGLSIELNDKKIITLIPKNKAAYKYTCIISSNLNEDVKLNDEEIFDLLKKYVDNNSFLITNVDNKNEFFNNAEIINTNDKKIFFNPIRYISDDEQDLYKGLFALKNNLTSTEANKIYKINEAYPYADEISQIFPNSEEIGKYISDNVNLNIFEDNEKHMAKFIAPDNMPSFSYLKQKCLAGLQHYFKNVKHEPIKEEYIQRLNHELSIIEKTGFADYFLIVHDYVSFARRNDIIVGPGRGSAAGSLVSFLLRITTIDPIEYNLLFERFLNPERITMPDIDIDFQDNRRDEVIEYLFEKYGKYNVATIVTYQTIAFKSAFRDACRIYQIDMNLVNLITKSLTDDYLSFDEILKTQKLIKEYSEKAEFKNIFKLVEKLIGCPRQTGTHAAGIIISDTDLRNVLPIRQGLNGIYQTQFDMNYLEDIGLIKMDLLGLRNLTTLKEILDLVKKNHNEDFTLSKIDLNDAKTFKILREGNTTGIFQLESQGMTNLIIDMKVNNINDISAASSLYRPGPQEMIPDYINNKNNRRVSLIDKSLANILLPTYGVIVYQEQVMQILQLVGNFSLGKADIVRRAMGKKQIEYMMEVKDEFINNAMANNYSNEKAVAIWNWIEQFAKYGFNKSHAIAYSYIGYWLAWFKAHYPAEFYTALLNGVVGNPSKTSRYIKEVKQFGIEVIKPSVKPAISKKLEDTIKLNYTSSENRIYMPLTLIKGIGRDFIATLNKVYEEHSNLFDDLNNFFFYMKNKGLNESNYTLLAKAGSFDCFGYNKSTMVANKDFIFSAMMAFNENISLDKQTKIENLFEEELNEEVESSYEKEVFGFYISSNPLTKLKNENAFFKPNDIIKLVENMNDVNIIGQISGIRVIKDKNQKNMAFVTVFDDTESIDLTVFSSNFENLQYDLVTNKNYVLKIRTQKYKNKITGILESIVKPV"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGAGCAATTT
     2041 CATCCCGCTG ATCAATGTGC GTAGCGTTTA TAATTTCCAA GAGTCCCTGA TTAAAATCAA
     2101 TGACTACATT AGCTTCGCAA AGAACGAGGG TTTCAAGCAC CTGTTCTACT GTGAAAACAA
     2161 AACGATGTAC GGCGTGGCTG AGTTCTTCCA GAAAGCGCTG AAGGAGAACA TCAAGCCGAT
     2221 TATTGGTCTG AGCATCGAAT TGAACGACAA AAAGATCATT ACGCTGATCC CGAAAAACAA
     2281 GGCAGCCTAC AAGTATACGT GCATTATCAG CAGCAACCTG AACGAGGATG TTAAGCTGAA
     2341 TGATGAAGAA ATTTTCGATC TGTTGAAGAA ATATGTGGAC AACAATAGCT TCTTGATTAC
     2401 CAACGTAGAC AACAAGAATG AGTTTTTCAA CAACGCGGAA ATCATCAATA CCAACGATAA
     2461 GAAAATTTTC TTCAATCCTA TTAGATATAT CAGCGACGAC GAGCAGGACC TGTACAAGGG
     2521 TCTGTTCGCA CTGAAGAACA ATCTGACCAG CACGGAGGCA AACAAAATCT ACAAGATTAA
     2581 TGAAGCATAC CCGTACGCAG ATGAAATCAG CCAGATCTTT CCGAACAGCG AGGAAATCGG
     2641 CAAATACATC AGCGACAATG TGAACCTGAA TATCTTTGAA GATAATGAGA AGCACATGGC
     2701 GAAGTTCATT GCGCCGGACA ATATGCCGTC CTTCTCTTAT CTGAAACAGA AGTGCCTGGC
     2761 TGGTCTGCAA CATTACTTTA AAAATGTGAA ACATGAGCCG ATTAAAGAGG AGTACATCCA
     2821 GCGCCTGAAC CACGAGCTGT CGATCATCGA AAAGACTGGC TTCGCCGATT ACTTCCTGAT
     2881 TGTTCATGAT TACGTTAGCT TTGCGCGTCG TAATGACATC ATTGTGGGTC CGGGTCGTGG
     2941 TAGCGCGGCC GGTTCGCTGG TCAGCTTTTT GCTGCGTATC ACCACGATCG ACCCGATCGA
     3001 ATATAATTTG CTGTTTGAGC GCTTCCTGAA CCCGGAACGC ATTACCATGC CAGATATCGA
     3061 CATTGACTTC CAGGACAATC GTCGCGACGA GGTTATTGAA TATTTGTTCG AAAAGTACGG
     3121 TAAATACAAT GTTGCCACGA TCGTTACCTA TCAAACGATC GCATTTAAAA GCGCGTTTCG
     3181 CGACGCCTGC CGTATCTATC AGATTGATAT GAATCTGGTT AACCTGATCA CTAAAAGCTT
     3241 GACGGATGAT TACTTGAGCT TCGATGAGAT CCTGAAAACC CAGAAGTTAA TCAAGGAGTA
     3301 TTCCGAGAAA GCTGAGTTTA AGAATATCTT TAAGTTAGTT GAGAAATTGA TCGGCTGCCC
     3361 ACGCCAGACC GGTACCCATG CAGCGGGTAT TATTATTAGC GATACCGATC TGCGTAACGT
     3421 GCTGCCTATC CGCCAGGGTC TGAACGGCAT TTACCAAACC CAGTTTGACA TGAACTATCT
     3481 GGAGGATATC GGCCTGATTA AGATGGATCT CCTGGGTCTG CGCAATCTGA CGACCCTGAA
     3541 AGAAATCCTG GACCTGGTTA AGAAGAACCA CAACGAAGAT TTCACCCTGT CCAAGATCGA
     3601 TCTGAATGAT GCAAAAACGT TTAAAATTCT GCGCGAAGGC AATACCACCG GTATTTTTCA
     3661 ATTGGAATCC CAAGGTATGA CGAACCTGAT CATTGATATG AAGGTGAATA ACATTAACGA
     3721 TATTAGCGCT GCCTCTAGCC TGTACCGTCC GGGTCCGCAG GAGATGATTC CGGACTATAT
     3781 TAACAACAAG AACAACCGCC GCGTGAGCCT GATCGATAAG TCTCTGGCTA ACATCCTGCT
     3841 GCCGACCTAC GGTGTTATTG TGTATCAAGA ACAGGTTATG CAGATCCTGC AACTCGTGGG
     3901 TAACTTCAGC CTGGGTAAGG CAGACATTGT CCGCCGTGCA ATGGGTAAGA AACAAATCGA
     3961 ATACATGATG GAAGTTAAGG ATGAGTTTAT TAATAACGCT ATGGCAAATA ATTACAGCAA
     4021 CGAAAAGGCG GTGGCAATTT GGAACTGGAT TGAGCAGTTT GCAAAATACG GTTTTAATAA
     4081 ATCGCATGCG ATTGCGTACA GCTACATCGG CTACTGGCTG GCTTGGTTTA AAGCGCATTA
     4141 CCCAGCTGAG TTCTATACGG CTTTGCTGAA TGGTGTGGTC GGTAATCCGA GCAAGACCAG
     4201 CCGCTATATC AAGGAAGTCA AGCAATTCGG CATCGAGGTT ATCAAACCGA GCGTGAAACC
     4261 GGCGATTAGC AAGAAGCTGG AGGACACGAT CAAACTGAAT TATACCAGCA GCGAAAATCG
     4321 TATCTATATG CCGCTGACCT TAATCAAAGG TATCGGTCGT GACTTTATCG CAACCCTGAA
     4381 TAAAGTCTAT GAAGAACATT CTAATCTGTT CGACGACCTG AACAATTTCT TCTTCTATAT
     4441 GAAGAACAAA GGCCTGAACG AATCTAATTA CACCCTGCTG GCTAAGGCGG GTAGCTTCGA
     4501 CTGTTTCGGT TACAACAAGA GCACTATGGT TGCCAATAAA GACTTCATCT TTTCCGCAAT
     4561 GATGGCGTTT AATGAGAATA TCAGCTTGGA TAAACAAACC AAAATTGAAA ATTTGTTTGA
     4621 GGAAGAACTG AACGAGGAGG TCGAGTCCAG CTACGAGAAG GAAGTGTTCG GCTTCTATAT
     4681 CTCGAGCAAT CCGCTGACCA AACTGAAGAA CGAGAACGCT TTTTTCAAGC CGAATGACAT
     4741 TATCAAGCTG GTGGAGAATA TGAACGACGT CAATATTATC GGTCAAATCA GCGGTATTCG
     4801 TGTTATCAAA GACAAAAATC AGAAGAATAT GGCGTTCGTT ACGGTCTTTG ACGATACGGA
     4861 GAGCATCGAT CTGACCGTTT TCAGCAGCAA TTTCGAGAAT CTGCAGTATG ATCTGGTGAC
     4921 GAACAAAAAT TATGTGCTGA AAATTCGTAC GCAAAAATAT AAAAACAAAA TCACTGGCAT
     4981 TCTGGAGTCA ATCGTCAAGC CGGTGTGAAG AGCTTAGAGA CCCCTACGGT TAAGGCTTGT
     5041 CT
//