LOCUS       Exported     3266 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3266
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3232
                     /label="Mfl584"
                     /note="Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS."
                     /translation="MKSILVRYGELTLKGNNKHMFISKLLDNIKYKLKKFDQSKIKFIKDNNSLVIDAEEEILEDVLEELKTVFGIYSLSVIEKCEKDLDKIAEKIIEIAKKSEYKRFKLEITRKDKSFPMTSADLKLALAPMVLKAVDNLIVDVHNPDLKIEVLVKKDGIQIFSKRIQGLKGLPVGVSGKGLSLLSGGIDSPVASFLTMKRGMHVDFIHFMTPPHTSPEALDKVFELAKIVSKYNSKKFNLYVCDFSMLLQELQHMPEESYKITIMRRMFMRIANKLAKQNGQEALITGESLGQVASQTIQSIDVINSTSELPILRPVLTYDKEEIILISKFIKSYETSILPFDDACSLFVPKQPVTKPKKWMAEQQENAILWNELLDYTMENKIQKFVFWNGSFTEEINKED"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCAAA TGAAAAGCAT
     2041 TCTGGTTCGT TACGGCGAGC TGACTTTAAA AGGCAACAAC AAACATATGT TTATCAGCAA
     2101 ACTGCTGGAC AACATCAAGT ATAAACTGAA AAAGTTCGAT CAGAGCAAGA TTAAATTTAT
     2161 TAAGGATAAC AATAGCCTGG TGATTGACGC GGAGGAGGAG ATCCTGGAGG ACGTTCTGGA
     2221 GGAACTTAAG ACCGTTTTCG GTATCTATAG CCTGTCTGTC ATTGAAAAGT GCGAAAAGGA
     2281 CCTGGATAAA ATTGCAGAAA AAATTATTGA GATCGCAAAG AAAAGCGAAT ACAAACGTTT
     2341 TAAGCTGGAA ATCACGCGTA AGGACAAATC GTTCCCGATG ACCAGCGCGG ATCTGAAGCT
     2401 GGCGCTGGCG CCAATGGTAC TGAAGGCGGT GGATAACTTA ATTGTTGATG TCCACAATCC
     2461 GGACCTGAAG ATTGAGGTGC TGGTGAAAAA AGACGGTATC CAGATTTTTA GCAAGCGTAT
     2521 CCAGGGCTTA AAGGGTTTGC CGGTTGGCGT TAGCGGTAAA GGCCTGAGCC TGCTGTCCGG
     2581 TGGTATCGAC AGCCCGGTGG CTAGCTTTCT CACCATGAAA CGTGGCATGC ACGTCGACTT
     2641 TATTCACTTT ATGACCCCGC CGCATACCAG CCCGGAGGCG CTGGACAAGG TTTTCGAGCT
     2701 GGCAAAAATC GTTAGCAAGT ACAACAGCAA AAAATTCAAC CTGTACGTCT GCGACTTCTC
     2761 TATGCTGCTG CAGGAACTGC AACATATGCC GGAGGAGAGC TACAAAATTA CCATTATGCG
     2821 TCGTATGTTT ATGCGTATCG CGAACAAGCT GGCGAAGCAG AATGGTCAAG AGGCCTTGAT
     2881 CACGGGTGAG AGCCTGGGCC AGGTGGCAAG CCAAACCATC CAGTCCATCG ATGTGATCAA
     2941 CAGCACGTCT GAACTGCCGA TCCTGCGCCC GGTGCTGACC TACGACAAAG AAGAGATCAT
     3001 TCTGATCTCC AAGTTTATTA AGAGCTATGA AACCAGCATC CTGCCTTTTG ACGATGCTTG
     3061 TAGCCTGTTT GTACCGAAAC AACCGGTGAC CAAGCCGAAG AAATGGATGG CAGAACAACA
     3121 GGAAAACGCG ATCCTGTGGA ATGAGCTGCT GGACTATACC ATGGAGAATA AAATTCAAAA
     3181 ATTCGTGTTC TGGAATGGTT CTTTCACGGA AGAAATCAAC AAGGAGGATT GAAGAGCTTA
     3241 GAGACCGGTA TATTTCTCCC CTCGGG
//