LOCUS       Exported     3854 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3854
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3820
                     /label="Mfl585"
                     /note=""
                     /translation="MILMSTLSNKPTALLIVGIVFVVISILGLLATLITLVLYKKTVEYTYESSRKLTYVDSSPLLKKIIIIYRIYIKNKDNRNLESTFYIMLRNYYNIFAERFVTLATETAEMFNENSYNPKPTIRNYLKAKKIFYRAEILHADTVDVINNIEQILEMQKIARDWYINVIEETNNIKSLIKAGNGSTNDTWSPNGLNSNFFNNLNLYIESAGLDIKNAKYNEAISNLKDAYEIISRLILILDDTEKIVYLIDYQLKNKLEEIRSSIFESTAGSELQKNNKKSQFNSISGFYANWVKTIKREIYSINIEKAYENSRRLLSQICLFEINMKYETSIKQFIESSQKVIKNAFSSISNEVVALKDIRNLNKKFGTNNNINSIKSRIFSVEQTIKDQEERYINLLNEFALSETSVQKLHYAKQGSNLMDIISKIFLSFENLRLIRAQLTSQEEIINEIQSRNISLKKVLVVMEVMIAKNPSISVLKTYQEQINNGFKILEKSEKNVRVYPDTFLSRPVFDEMIEYLDGKIVSFAILKNDMASTIYLAKLAEVSLSYLNRFGGIESIDKNIAKSLEKLKQENYTECLKDNVLILNKLNSQNIAVS"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATGCAAAG CTATTAGCGC GGGTCTCAAA TGATCCTGAT
     2041 GAGCACCCTG TCGAATAAGC CGACGGCACT GCTGATCGTT GGCATTGTGT TTGTGGTGAT
     2101 TAGCATTTTG GGCTTGCTGG CCACCTTGAT TACGCTGGTC CTGTACAAGA AAACTGTGGA
     2161 ATATACGTAC GAGTCCAGCC GTAAATTGAC GTACGTAGAC TCTAGCCCTC TGCTGAAGAA
     2221 AATTATTATC ATTTACCGTA TTTACATTAA GAACAAGGAT AACCGCAACC TGGAGAGCAC
     2281 CTTCTACATC ATGTTGCGCA ACTACTACAA TATTTTTGCT GAGCGTTTCG TTACCTTGGC
     2341 GACCGAAACG GCCGAGATGT TCAATGAAAA TAGCTATAAC CCGAAACCGA CCATTCGTAA
     2401 CTATTTGAAG GCGAAGAAGA TCTTTTACCG TGCAGAAATC CTGCATGCCG ATACCGTTGA
     2461 CGTCATTAAC AACATCGAGC AGATCCTGGA AATGCAGAAA ATCGCGCGTG ATTGGTATAT
     2521 CAACGTTATT GAGGAAACGA ACAACATTAA GAGCCTGATC AAAGCCGGTA ACGGTTCTAC
     2581 CAATGACACG TGGAGCCCGA ACGGCCTGAA TAGCAATTTC TTCAACAACT TGAATCTGTA
     2641 CATCGAAAGC GCCGGTTTGG ACATCAAAAA CGCAAAATAT AATGAAGCCA TTAGCAATCT
     2701 GAAGGACGCA TATGAAATTA TCAGCCGCTT AATCCTGATT CTGGACGATA CCGAAAAGAT
     2761 TGTGTACCTG ATTGATTATC AGCTGAAGAA CAAACTGGAG GAAATCCGTT CTAGCATCTT
     2821 TGAAAGCACC GCGGGTAGCG AGCTGCAGAA AAACAACAAA AAGAGCCAGT TTAATAGCAT
     2881 TAGCGGCTTT TACGCAAACT GGGTCAAGAC GATCAAGCGT GAAATTTACA GCATTAACAT
     2941 CGAGAAAGCG TACGAAAACA GCCGTCGTTT ACTGAGCCAA ATCTGCCTCT TCGAAATTAA
     3001 TATGAAATAC GAAACTTCTA TCAAACAGTT TATCGAAAGC TCTCAGAAAG TTATTAAGAA
     3061 TGCATTCAGC AGCATTTCCA ACGAGGTGGT GGCCCTGAAG GACATCCGCA ATCTGAACAA
     3121 AAAATTTGGT ACTAACAACA ATATTAACAG CATCAAAAGC CGTATTTTTA GCGTCGAGCA
     3181 AACCATCAAA GATCAGGAGG AGCGTTACAT TAATCTGCTG AATGAGTTTG CCCTGAGCGA
     3241 AACCAGCGTC CAAAAACTGC ACTACGCGAA GCAGGGTAGC AACCTGATGG ATATTATCTC
     3301 GAAGATTTTC CTGAGCTTCG AGAATCTGCG TCTGATTCGT GCGCAGCTGA CCAGCCAAGA
     3361 AGAAATCATC AATGAGATCC AATCTCGTAA CATCAGCCTG AAGAAAGTTC TGGTGGTCAT
     3421 GGAAGTCATG ATCGCAAAAA ACCCGAGCAT TAGCGTTCTT AAGACCTATC AGGAGCAAAT
     3481 TAACAACGGT TTTAAGATTC TGGAAAAGAG CGAGAAAAAC GTGCGCGTGT ACCCGGATAC
     3541 CTTTCTGAGC CGTCCGGTGT TCGATGAGAT GATTGAATAC CTGGATGGTA AAATTGTTAG
     3601 CTTCGCAATT CTGAAAAATG ATATGGCGAG CACCATCTAC CTGGCGAAAC TGGCGGAAGT
     3661 CAGCCTGTCG TACCTGAATC GCTTCGGTGG CATCGAATCG ATTGATAAAA ATATTGCAAA
     3721 GAGCCTGGAA AAGCTGAAGC AGGAAAATTA TACCGAATGC CTGAAGGATA ATGTTTTGAT
     3781 CCTGAATAAA CTGAACAGCC AGAACATTGC AGTTTCCTGA AGAGCTTAGA GACCGGGTGC
     3841 CTTGCTAATC TAGT
//