LOCUS Exported 3304 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3304 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1342..1389 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3237..3253 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3254..3285 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1270 /label="Mfl587" /note="Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)." /translation="MNIIKELEWRGLVKQITNEERLLKAQNDGAAVYCGFDPTADSLHVGHLMMIVTLKRFDKAGFQAIGLIGGGTGMIGDPSFKADERKLQTDEQVKFHATAIQNQLLRIIPDVTFANNVDWLGNMSLIDFLRDVGKDFNISYLLNKDSIATRISTGLSVTEFSYTMLQAYDFYNLYINHNCKVQIGGSDQWGNITSGTDYISTRVGSANTEAAGFTIPLLTKSDGQKFGKTESGAVWLDSNKTSVYDFYQFWINQDDNDCVKMLKYLTFLTKEEIDELEAKHKEAPHLRTMQKTLASEITKFVHGDKELNKAIKLTDAFFTGNILNLDDDLLELAIKSIPTIQLEKTTSAIDAIVNVNAASSKREAREFINAKAISFNDVAVQDENMLLSEIKTIKNNKIIVKKGKKKYYLLEIK" primer_bind complement(1394..1410) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1477..2065) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2256..3116) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3117..3221) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGACTGCA TACCAGGTTG GGTCTCAAAT GAACATTATT AAGGAGCTGG AATGGCGCGG 61 TCTGGTCAAG CAAATTACGA ATGAGGAGCG TCTGCTGAAG GCCCAGAACG ATGGTGCGGC 121 CGTCTACTGT GGCTTTGACC CGACCGCAGA CAGCCTGCAC GTCGGCCACT TGATGATGAT 181 CGTTACCCTG AAACGTTTCG ACAAGGCGGG TTTCCAAGCC ATCGGTCTGA TTGGCGGCGG 241 CACCGGTATG ATCGGTGATC CGAGCTTCAA GGCGGATGAA CGTAAGCTGC AGACGGACGA 301 ACAGGTCAAA TTTCACGCGA CGGCCATTCA GAACCAGCTG CTGCGCATTA TTCCTGATGT 361 GACTTTTGCA AATAACGTCG ATTGGCTGGG CAATATGTCA CTGATCGATT TTCTGCGTGA 421 CGTCGGTAAA GATTTCAATA TTTCTTATCT GCTGAACAAA GATAGCATTG CCACCCGCAT 481 CAGCACCGGT TTGAGCGTGA CGGAGTTTAG CTACACCATG CTGCAGGCAT ACGATTTCTA 541 TAATCTGTAC ATTAACCACA ATTGTAAAGT TCAGATTGGT GGCAGCGATC AATGGGGTAA 601 CATTACGAGC GGTACCGATT ATATTTCGAC GCGCGTTGGT AGCGCGAATA CCGAGGCAGC 661 CGGTTTCACT ATCCCTCTCC TGACCAAATC TGATGGTCAG AAGTTCGGCA AAACCGAGTC 721 CGGTGCGGTG TGGCTGGATA GCAATAAGAC GAGCGTCTAC GATTTCTATC AGTTTTGGAT 781 TAATCAAGAT GATAATGACT GTGTGAAAAT GTTGAAGTAC CTGACCTTTC TGACTAAGGA 841 GGAAATCGAC GAACTGGAGG CGAAACACAA AGAGGCCCCG CATTTGCGCA CCATGCAAAA 901 GACGCTGGCG TCGGAGATTA CCAAGTTCGT TCACGGTGAC AAGGAGCTGA ATAAAGCAAT 961 CAAGCTGACC GATGCATTCT TCACCGGTAA TATCCTCAAC CTGGACGACG ATTTGCTGGA 1021 GCTGGCTATC AAAAGCATTC CGACGATCCA ACTGGAAAAG ACTACCAGCG CGATTGACGC 1081 CATCGTCAAT GTCAACGCCG CCTCCAGCAA ACGCGAAGCA CGTGAGTTCA TCAACGCAAA 1141 GGCGATTTCA TTCAATGATG TCGCGGTTCA GGATGAGAAC ATGCTGCTGA GCGAGATTAA 1201 AACGATCAAG AATAACAAGA TCATCGTTAA AAAAGGCAAG AAAAAATACT ATCTGCTGGA 1261 AATTAAGTGA AGAGCTTAGA GACCGGCGGT GTCTACATAT CCTTCGCTGA GGTGTCAATC 1321 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 1381 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 1441 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 1501 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 1561 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 1621 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 1681 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 1741 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 1801 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 1861 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 1921 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 1981 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 2041 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC 2101 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 2161 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 2221 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 2281 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 2341 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA 2401 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 2461 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 2521 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 2581 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 2641 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 2701 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 2761 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 2821 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 2881 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 2941 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 3001 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 3061 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 3121 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 3181 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA 3241 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG 3301 GGAG //