LOCUS       Exported     3118 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3118
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1156..1203
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3051..3067
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3068..3099
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1084
                     /label="Mfl588"
                     /note=""
                     /translation="MNKINFDKKIKDGYYIADYFKKTSLIINNEFPNSIVTMQFFQRKNNITLSGINEVIQLLEFACKDYKNLKIWALKDGDIINANEPVLKIEGNYSSFGWLEGMIDGILARNSCIATNSSKIVKAANGKPVLNMMDRADSYLTLPSDGYSSWVGGIKLFVTEASVELIDDSSVPKPSGTMPHALIQAFDGDILKATEAFYRNFPESNLLSLVDYNNDCVRDSVKVANHFKEKLWAIRIDTSGNLIDKYLEENRNKYPENANLYGVNQYLIKEVRKALDESGNKHVKIIVSSGLTEEKIKEFENNNTPVDIYGVGGYLAKIDNNFTGDAVLINGKEQAKFGRKNIASDRLTQIK"
     primer_bind     complement(1208..1224)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1291..1879)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2070..2930)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2931..3035)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAATAAAATT AACTTCGACA AGAAAATCAA
       61 GGACGGTTAC TACATTGCGG ACTATTTCAA AAAGACCAGC CTGATTATTA ATAACGAATT
      121 TCCGAATTCA ATTGTAACCA TGCAGTTCTT CCAACGTAAG AACAACATCA CCCTGAGCGG
      181 TATTAACGAG GTGATCCAGC TGCTTGAGTT TGCCTGCAAA GACTACAAAA ATCTGAAAAT
      241 TTGGGCGTTA AAAGACGGTG ATATCATCAA TGCAAACGAA CCGGTGCTGA AGATTGAGGG
      301 CAATTATAGC TCCTTTGGCT GGCTGGAGGG TATGATTGAT GGCATCCTGG CGCGCAACTC
      361 TTGTATCGCG ACCAACAGCT CCAAGATTGT CAAGGCCGCA AACGGTAAGC CGGTGCTGAA
      421 CATGATGGAC CGTGCAGACA GCTACCTGAC CCTGCCGAGC GACGGTTATA GCAGCTGGGT
      481 TGGTGGCATC AAGTTGTTCG TGACGGAAGC GTCTGTCGAG CTGATCGACG ACAGCAGCGT
      541 CCCAAAACCG AGCGGTACCA TGCCGCATGC GCTGATCCAA GCGTTTGATG GTGATATCCT
      601 GAAAGCGACG GAAGCTTTTT ACCGTAATTT CCCGGAAAGC AATCTGCTGT CTCTGGTCGA
      661 TTACAATAAT GACTGCGTGC GTGACAGCGT GAAGGTCGCG AACCACTTTA AGGAAAAGCT
      721 GTGGGCGATC CGTATTGACA CCTCCGGTAA TCTGATCGAC AAGTATCTGG AAGAGAACCG
      781 TAACAAGTAC CCGGAAAACG CGAATCTGTA CGGTGTCAAC CAATACCTCA TTAAGGAGGT
      841 CCGTAAGGCA TTGGACGAGA GCGGTAACAA ACATGTTAAA ATTATCGTTA GCAGCGGCCT
      901 CACCGAGGAG AAAATTAAAG AGTTCGAGAA CAATAACACG CCGGTCGACA TTTACGGCGT
      961 CGGTGGTTAC CTGGCTAAAA TCGACAATAA TTTTACTGGT GACGCGGTGC TGATCAACGG
     1021 TAAAGAGCAA GCTAAATTCG GCCGTAAGAA TATCGCCAGC GACCGCCTGA CCCAAATCAA
     1081 GTGAAGAGCT TAGAGACCGG CGGTGTCTAC ATATCCTTCG CTGAGGTGTC AATCGTCGGA
     1141 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1201 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1261 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1321 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1381 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1441 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1501 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1561 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1621 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1681 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1741 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1801 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1861 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1921 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1981 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2041 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2101 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2161 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2221 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2281 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2341 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2401 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2461 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2521 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2581 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2641 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2701 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2761 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2821 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2881 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2941 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3001 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3061 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//