LOCUS Exported 3193 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3193 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1231..1278 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3126..3142 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3143..3174 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1159 /translation="MLNQIKEEVNTDILVENIISNANQSFLAPEIVFINKPYTINKNSYFKMLVSLNLNPNMVDVDEYKKIYGITNLDFKLYNSDLKEIKPASTITKNYEINNKSKIFFEFEIPISLKEEHAINLSFGFSHEFKEIKYKSKGFIKINPTFNKVDNEININLINYLTYYDIKNEIKKWTSDCILSANLNLNFNQLEVNKYYKNFAKMTISFNEENQHLINNNKIILEEIQQAKNNKIFPHLILEDFKINDENDFKNYYSGKLTAKLVENENLFLINYDGLSVLDEKTKKVLLNEGLDGLYINPYNWNLDQCMVMRLNIFGETFTFNSIIKSYNKYQFIDNLKFNIQNSKKFIYKFDKLFWTILILLNAEEIKEMIKGYEIF" /label="Mfl595" /note="" primer_bind complement(1283..1299) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1366..1954) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2145..3005) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3006..3110) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GCTGAATCAG ATTAAGGAGG AGGTTAACAC 61 CGACATCCTG GTTGAGAACA TTATCAGCAA TGCAAATCAG AGCTTCCTGG CACCTGAAAT 121 TGTTTTCATT AACAAGCCGT ACACCATTAA CAAAAACTCC TACTTCAAAA TGCTGGTTAG 181 CCTGAATCTG AACCCGAATA TGGTTGATGT TGATGAGTAC AAGAAAATTT ACGGTATTAC 241 CAATTTGGAC TTCAAATTGT ATAACTCTGA CCTGAAAGAA ATCAAGCCGG CGTCTACCAT 301 TACTAAAAAC TATGAGATCA ACAACAAGAG CAAGATCTTT TTTGAGTTTG AGATCCCGAT 361 CTCTCTGAAA GAGGAACACG CGATCAACCT GAGCTTTGGT TTCTCGCACG AGTTCAAGGA 421 GATCAAATAC AAGAGCAAAG GTTTCATCAA GATTAACCCG ACCTTTAACA AGGTCGATAA 481 TGAAATTAAT ATCAATTTGA TCAACTACCT GACGTACTAT GATATCAAGA ACGAAATTAA 541 GAAATGGACC TCCGATTGTA TCCTGAGCGC GAATCTGAAT CTGAACTTCA ACCAATTGGA 601 GGTGAACAAA TACTACAAGA ACTTCGCGAA GATGACCATT AGCTTCAACG AAGAAAATCA 661 GCACTTAATC AATAACAATA AAATCATCCT GGAGGAGATC CAGCAGGCAA AAAACAATAA 721 AATCTTTCCG CATCTGATTC TGGAGGACTT TAAGATTAAT GACGAGAACG ATTTCAAGAA 781 TTATTATTCG GGTAAACTGA CTGCGAAACT GGTTGAGAAC GAGAATCTGT TTTTGATTAA 841 TTATGACGGC CTGTCTGTGC TGGATGAAAA GACCAAGAAA GTTTTGCTGA ATGAGGGCCT 901 GGATGGTTTG TACATTAATC CGTACAACTG GAACCTGGAT CAATGCATGG TCATGCGTTT 961 AAACATTTTC GGCGAAACGT TCACCTTCAA CTCCATCATC AAAAGCTACA ACAAGTACCA 1021 GTTTATTGAT AACCTTAAAT TCAACATCCA AAACAGCAAA AAATTCATCT ATAAGTTCGA 1081 TAAACTGTTC TGGACCATCC TGATCCTGCT GAACGCGGAG GAAATCAAAG AGATGATTAA 1141 GGGCTACGAG ATCTTTTGAA GAGCTTAGAG ACCGGTTCCT AGACCTCCTG GATCGCTGAG 1201 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 1261 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 1321 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 1381 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 1441 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1501 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1561 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1621 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1681 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1741 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1801 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 1861 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 1921 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 1981 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 2041 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 2101 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 2161 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 2221 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 2281 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 2341 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 2401 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 2461 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 2521 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2581 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2641 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 2701 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 2761 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 2821 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 2881 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 2941 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 3001 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 3061 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 3121 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA 3181 GGTGGAGTGG GAG //