LOCUS Exported 3173 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3173 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3139 /label="Mfl600" /note="" /translation="MKNIEQKINEFISNNFYPGAVIKIDINGKDLYYSSFGYSDLENKKPMKKNQIFPIYSMTKPIVVVACLLLIQDGLLSLDTKVSDFYSNFNQNMKIKNLLNMTSGITYSWSAKNNIEKQIEIEEEIETNNYNLHEIIEMISKIPTEIEPGADWIYGMGIDVLGGIIEKVSGIKLSTFLKEKIFYPLKMNDTDFKIKDLNRKPVKYNLDSSNGYKLIRNENYHWMMPEDLTKIPNCCLGGSGVFTTASDYNKFLNFLLCGKIENKEYLDTKYLNEMTSNQITNLKNIKIFDLNEDYQYGYGVRVRTKNNVFPLTEVGEFGWGGVLGTTSLADPKNKVVMSFMVSVYPGNNMLVEKELFDALYKDLKDKKLI" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGACTGC ATACCAGGTT GGGTCTCAAA TGAAAAATAT 2041 CGAACAAAAG ATCAATGAAT TCATTAGCAA TAACTTCTAT CCAGGCGCAG TCATCAAGAT 2101 CGATATTAAC GGCAAGGACC TGTATTACAG CTCCTTTGGT TACAGCGACC TGGAGAACAA 2161 AAAGCCGATG AAGAAAAATC AGATCTTCCC GATCTATTCG ATGACTAAAC CGATCGTTGT 2221 GGTGGCATGC CTGCTGCTGA TCCAAGATGG TCTGCTGAGC CTGGACACCA AGGTGTCGGA 2281 CTTTTACAGC AATTTTAACC AAAACATGAA GATCAAAAAT CTGCTGAATA TGACTAGCGG 2341 TATTACGTAT AGCTGGTCTG CGAAAAACAA TATCGAAAAA CAGATTGAAA TTGAAGAGGA 2401 GATTGAAACC AACAATTACA ACCTCCACGA GATCATTGAG ATGATTAGCA AGATTCCGAC 2461 CGAAATCGAA CCGGGTGCAG ATTGGATCTA CGGTATGGGT ATCGATGTGC TGGGCGGTAT 2521 CATCGAAAAG GTTAGCGGTA TTAAGCTGTC AACTTTTTTG AAAGAGAAAA TTTTCTATCC 2581 GCTGAAGATG AATGACACCG ACTTTAAAAT TAAGGACCTG AATCGTAAAC CGGTGAAGTA 2641 CAATCTGGAT TCTAGCAACG GCTATAAACT GATCCGCAAC GAAAATTATC ACTGGATGAT 2701 GCCGGAGGAC CTGACGAAAA TTCCGAATTG CTGCCTGGGC GGCAGCGGTG TTTTTACGAC 2761 GGCGTCCGAC TACAACAAGT TCCTGAATTT CCTGCTGTGC GGTAAAATCG AGAACAAAGA 2821 GTATTTGGAT ACCAAGTATC TGAACGAGAT GACGTCTAAC CAAATCACCA ATCTGAAAAA 2881 CATTAAGATC TTTGACCTGA ATGAGGACTA TCAGTATGGT TACGGTGTTC GTGTGCGTAC 2941 GAAAAATAAT GTCTTTCCTC TGACCGAAGT CGGTGAATTT GGTTGGGGTG GCGTGCTGGG 3001 TACGACGTCA CTGGCTGATC CTAAAAACAA GGTTGTTATG TCCTTCATGG TTAGCGTCTA 3061 CCCGGGCAAT AATATGCTGG TTGAAAAGGA GCTGTTCGAC GCGTTGTATA AAGACCTGAA 3121 GGATAAAAAG CTGATTTGAA GAGCTTAGAG ACCCCGAGAT TTAGACGGAT GCT //