LOCUS       Exported     3451 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3451
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1489..1536
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3384..3400
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3401..3432
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1417
                     /label="Mfl605"
                     /note=""
                     /translation="MKLNKKYGFWTVLASTLAAIVGSSIIISFNMVFALSEANPLLMILAWVFGALIVLPDAFIVIEPSIGYGESGSGYSWIRKCNWRILAFWFGWVLILFVSATSLASCCSAMSSMITQILELNPEEIAVETLQKSLAIFILIFLAGIQIMIKNSSKYTQLFFLFVKTLPIILVFILAIMYGSKDGLLSNSEMNKNLGHAYISSAMLIPAITYTGFAYSGHEFPTYITEEIENPKKTIPWVIISAVLIVLVIYVCYGIALLSLATPDSNGNWINPGGTTSSIFAQHRWAVLTFNIFAIFLFIGSVNSLLFFQSRLIHKLSETGDVHSVFGKVHKKTNQPYMAIILLCCVAVFYILFSSISEIISSFALATSVLKILLNSSIIKLRLKDPEYKKIYGNKTFWTLMILSLVTCALTFIGSIYLMVIMPHQQTGASTFSILWKPILMVLIAFLVYLFGIFKFNNIKIK"
     primer_bind     complement(1541..1557)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1624..2212)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2403..3263)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3264..3368)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAAGCTGAAT AAAAAGTATG GTTTCTGGAC
       61 CGTCCTGGCT TCCACGCTGG CGGCGATCGT TGGTTCTAGC ATCATCATTA GCTTTAACAT
      121 GGTGTTCGCT CTGAGCGAGG CGAATCCGCT GCTCATGATT TTGGCGTGGG TGTTCGGCGC
      181 GCTGATCGTT CTTCCGGATG CCTTTATCGT GATCGAGCCG AGCATCGGTT ACGGTGAGTC
      241 TGGTAGCGGT TACTCCTGGA TTCGTAAATG CAACTGGCGT ATTCTGGCGT TTTGGTTTGG
      301 TTGGGTCCTG ATTCTGTTTG TGAGCGCGAC CAGCCTGGCG TCCTGCTGTA GCGCTATGAG
      361 CAGCATGATC ACGCAAATTC TGGAACTGAA TCCGGAAGAG ATCGCAGTGG AAACCCTTCA
      421 GAAAAGCCTG GCTATCTTTA TCCTGATCTT CCTGGCCGGT ATCCAGATCA TGATTAAGAA
      481 TTCTTCTAAA TATACGCAAC TGTTTTTTCT GTTCGTTAAG ACCCTGCCGA TCATCCTGGT
      541 CTTTATCCTG GCGATTATGT ATGGCTCAAA AGATGGCCTG TTGTCCAATA GCGAGATGAA
      601 TAAAAACTTG GGTCACGCCT ACATTAGCTC GGCTATGCTG ATTCCGGCAA TTACGTACAC
      661 CGGCTTTGCT TATTCTGGTC ACGAGTTCCC GACGTACATT ACTGAGGAAA TTGAGAACCC
      721 GAAGAAAACT ATTCCGTGGG TGATCATTAG CGCAGTCCTG ATCGTCCTGG TTATTTATGT
      781 CTGCTATGGT ATTGCGCTGC TGAGCCTGGC GACCCCGGAC AGCAATGGTA ACTGGATTAA
      841 CCCAGGTGGT ACGACCAGCA GCATCTTTGC ACAGCACCGT TGGGCCGTGC TGACCTTCAA
      901 TATCTTCGCT ATCTTTTTGT TCATTGGCAG CGTGAATAGC CTGCTGTTCT TCCAATCCCG
      961 TCTGATCCAC AAGCTGAGCG AAACTGGCGA CGTTCATTCC GTGTTCGGTA AGGTCCACAA
     1021 GAAAACGAAC CAACCGTACA TGGCAATTAT CTTGCTGTGT TGTGTGGCGG TGTTCTACAT
     1081 CCTTTTTAGC TCGATTTCGG AGATCATCTC GTCCTTCGCG CTGGCGACCA GCGTTCTGAA
     1141 GATTCTGCTG AACAGCAGCA TCATCAAACT GCGCCTGAAA GATCCGGAGT ATAAAAAGAT
     1201 TTACGGCAAC AAGACGTTCT GGACGCTGAT GATCCTGTCT CTGGTGACGT GCGCTCTGAC
     1261 CTTTATTGGT TCCATCTATC TGATGGTGAT CATGCCGCAT CAGCAAACCG GTGCAAGCAC
     1321 GTTCAGCATT TTATGGAAGC CAATCCTGAT GGTCCTGATC GCCTTCCTGG TCTATCTGTT
     1381 CGGCATCTTT AAATTCAACA ACATTAAGAT CAAGTGAAGA GCTTAGAGAC CGTGTCGCTC
     1441 GCAATCCTAA TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1501 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1561 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1621 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1801 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     2041 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2221 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2281 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2341 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2401 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2461 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2521 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2581 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2641 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2701 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2761 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2821 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2881 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2941 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     3001 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     3061 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3121 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3181 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3241 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3301 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3361 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3421 AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA G
//