LOCUS       Exported     3085 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3085
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1123..1170
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3018..3034
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3035..3066
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1051
                     /label="Mfl606"
                     /note=""
                     /translation="MRFTLEKINKYKYSLKTVLITLLQMKSIKKNSSSHFLDYKNFCYNYYRDGFTKEGIKVNSLDIYYDDLKNKNLNYLTDTFNEKSIEEFDLFNSNGKISCLVAKNANSKKWVVGLHGWTENKFLALRLVHHFWKQGYNILTFDAFAHGLSYGEKTDIGYSSISMLETIVEHLKNEEQADSIGLIGNSMGASTSILFVQKNKLRSKINWVVADCGFSNIKLQYRYYIENNLYHVPWWKIGFLFTHKFSKETKTKQRKYNLLLNMKKAKSNPIFFVHSKGDTFIPYEMSQIMYEKKIKYEKVKISKIWTPEGSEHVNTIKTYNDDYILKTINFARKWEVNETK"
     primer_bind     complement(1175..1191)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1258..1846)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2037..2897)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2898..3002)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GCGTTTCACC CTGGAGAAAA TCAACAAATA
       61 TAAGTACTCG TTGAAAACGG TTCTGATTAC CCTGCTGCAA ATGAAAAGCA TCAAAAAGAA
      121 CAGCAGCAGC CATTTCCTGG ACTATAAAAA TTTCTGTTAT AACTATTATC GTGATGGTTT
      181 TACGAAGGAA GGTATTAAAG TGAACTCGCT GGACATCTAT TATGACGACT TGAAGAATAA
      241 AAATCTGAAC TACCTGACCG ATACTTTTAA CGAGAAAAGC ATCGAAGAGT TCGATTTGTT
      301 CAACAGCAAC GGTAAAATTA GCTGCCTGGT CGCAAAGAAC GCGAATAGCA AAAAATGGGT
      361 GGTCGGCCTG CACGGCTGGA CCGAAAATAA ATTTCTGGCG CTGCGTCTGG TTCATCATTT
      421 CTGGAAACAA GGTTACAACA TCCTGACTTT TGATGCATTT GCGCACGGTT TGAGCTACGG
      481 TGAGAAAACG GACATTGGTT ATTCTTCTAT TAGCATGCTG GAGACTATTG TTGAACATCT
      541 GAAGAATGAG GAGCAGGCAG ACAGCATCGG TCTGATCGGC AATAGCATGG GTGCCAGCAC
      601 GTCAATCCTT TTCGTGCAGA AAAATAAACT GCGTAGCAAG ATTAACTGGG TGGTTGCCGA
      661 CTGTGGTTTT AGCAACATTA AGCTGCAATA CCGTTACTAC ATTGAAAATA ATCTGTACCA
      721 CGTCCCGTGG TGGAAAATTG GTTTCCTGTT TACCCACAAG TTCAGCAAGG AAACGAAAAC
      781 TAAACAACGC AAGTACAATC TGCTTCTGAA CATGAAGAAA GCCAAGTCGA ACCCGATCTT
      841 TTTTGTTCAC AGCAAAGGCG ATACGTTTAT TCCGTACGAA ATGAGCCAGA TTATGTACGA
      901 AAAGAAGATT AAGTACGAAA AGGTGAAGAT TAGCAAGATC TGGACGCCAG AGGGTAGCGA
      961 GCACGTGAAT ACCATCAAAA CTTACAATGA TGACTATATC CTGAAAACCA TCAACTTCGC
     1021 CCGCAAATGG GAGGTAAATG AAACCAAGTG AAGAGCTTAG AGACCGGCCT TTTATTGGGT
     1081 CCAGTCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1141 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1201 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1261 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1321 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1381 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1441 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1501 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1561 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1621 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1681 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1741 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1801 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1861 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1921 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     1981 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2041 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2101 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2161 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2221 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2281 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2341 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2401 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2461 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2521 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2581 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2641 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     2701 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     2761 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     2821 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2881 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     2941 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3001 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3061 TTGACTTGGT CAGGTGGAGT GGGAG
//