LOCUS       Exported     4688 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4688
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4654
                     /label="Mfl619"
                     /note=""
                     /translation="MEIKIYAPVDCEVKSLSECSDETFSKKLLGDGLLIIPKKNKFYSPFLEAKTIMIFETKHAYGFDIEGINVLIHCGLDTVKLGGKPFKTNLEIDKKIRLGDEIFEVDLKMVNAEKISTETPIVFDQPIEILNSVKGNFKQGDLICTIKVLEEQIVKADPNKMTNKDFEEFFNAENKYQKEARILNELVGGPKNYRDVYNCMTRLRFLVKDKEIVNEEKIRTLSLVKSTIWQGDELQVVIGQDVYKLKDEVIAQNEFANSVVASQTNENNEKQSRGAQFIRMFASIMVKTIPVLVGCAIVQAIVGILVQINVMPDIVITAQASGNQVLLKDAAIGWIILFIMAKTTTVFGTIAIAISTAQYFKFDVIIAASIALILSTPLMFLDGGSGGMGHEWIVIDFGDLNTGNPVLDGISKVKIAAMTNKMFVVIGATIAAKYLNDWIKTWIPISLELMFRPFLLVMVIVPTSFFILLPVWNVIETLAGTLMYWIGQAPLGIGVGFYIGIWQVAVIFGIHMGLIIVGILDSIQRGGAGIFMIMGISVWAQVGALVGVILVTQNSKLKKDAIHMLPAGCLGITEPILYGISLPKKRPLIAGCIAAFFAGAYCNAVGVTARAGTGFGIFEFIGFFSSPTMGGTAELSNISNGLLYISGAAIALALGTVFSLLIYIERPNEKSSISKSANALLKFIKVKDNLTDEEIQILKSQIKEMKAVVNKDTVKQIKKIEKQIQKVVSVDSKIETLTENEYKHEQQIYKKGKKAISKNDLQTSKKLVNEFNSLSYKAKIDKLKIERDELKAKIDFKTLDKIIEPKQKEIDNLLSQISKQYNLKEEIVEIKNNYWNDLNSLKIAYDFENPKNINISLKQLTKSLKKNKKAVKTI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GACAACGAGC AGACCGAATA GGGTCTCAAA TGGAGATTAA
     2041 GATCTACGCC CCAGTTGACT GCGAGGTTAA GAGCCTGAGC GAGTGTTCGG ATGAAACGTT
     2101 CAGCAAAAAA TTGCTGGGTG ACGGCCTGCT GATTATTCCG AAGAAAAATA AATTTTACAG
     2161 CCCGTTTCTG GAAGCCAAGA CGATTATGAT CTTCGAAACC AAACATGCAT ACGGCTTTGA
     2221 CATCGAAGGT ATCAACGTTC TGATCCACTG TGGTCTGGAT ACGGTCAAGC TCGGTGGTAA
     2281 ACCGTTCAAA ACCAACCTGG AGATTGACAA GAAGATTCGC CTCGGTGATG AAATCTTCGA
     2341 GGTTGACCTG AAAATGGTGA ATGCAGAAAA GATTAGCACC GAAACCCCGA TCGTGTTTGA
     2401 TCAACCGATT GAGATCCTGA ACAGCGTGAA GGGTAACTTT AAACAGGGTG ACCTGATTTG
     2461 CACGATCAAG GTCCTGGAGG AGCAGATCGT TAAGGCAGAC CCGAACAAAA TGACCAATAA
     2521 AGACTTTGAG GAGTTCTTTA ATGCGGAAAA CAAATATCAA AAAGAAGCCC GTATTCTGAA
     2581 CGAGCTGGTT GGCGGCCCAA AAAATTATCG TGATGTATAT AACTGCATGA CCCGCCTGCG
     2641 TTTTCTGGTC AAGGATAAGG AGATCGTTAA CGAAGAAAAG ATTCGCACCC TCAGCCTCGT
     2701 GAAAAGCACC ATCTGGCAAG GCGACGAGCT GCAAGTGGTT ATCGGTCAGG ATGTGTACAA
     2761 ACTGAAAGAT GAGGTGATCG CGCAGAACGA GTTTGCGAAT AGCGTGGTGG CCAGCCAGAC
     2821 TAATGAGAAC AATGAGAAAC AGAGCCGTGG CGCCCAGTTT ATCCGCATGT TCGCAAGCAT
     2881 TATGGTTAAA ACCATCCCGG TGCTGGTGGG TTGCGCGATC GTCCAAGCCA TTGTTGGTAT
     2941 TCTGGTTCAG ATTAACGTAA TGCCGGATAT TGTCATCACT GCGCAGGCGA GCGGTAATCA
     3001 AGTGCTGCTG AAAGATGCAG CCATCGGTTG GATCATTCTG TTTATCATGG CAAAGACCAC
     3061 CACGGTTTTC GGCACCATTG CGATTGCGAT TAGCACGGCG CAATACTTTA AGTTCGATGT
     3121 GATTATCGCG GCATCAATCG CTCTGATCCT GAGCACGCCG CTGATGTTCC TGGACGGTGG
     3181 TAGCGGTGGT ATGGGCCATG AGTGGATCGT AATCGACTTC GGTGATCTTA ACACGGGCAA
     3241 TCCAGTTCTG GACGGCATCA GCAAGGTGAA GATTGCCGCC ATGACCAACA AGATGTTTGT
     3301 TGTTATCGGT GCTACCATTG CGGCGAAGTA CCTGAACGAT TGGATTAAAA CTTGGATCCC
     3361 GATTTCTCTG GAACTGATGT TCCGCCCGTT TCTGCTGGTT ATGGTCATTG TTCCGACGAG
     3421 CTTCTTTATT CTGCTGCCGG TTTGGAATGT TATTGAAACG TTAGCGGGCA CCTTGATGTA
     3481 CTGGATTGGT CAAGCGCCGT TGGGCATTGG CGTTGGTTTT TATATTGGTA TTTGGCAAGT
     3541 TGCTGTCATT TTCGGCATCC ATATGGGTCT GATTATTGTG GGCATCCTGG ACAGCATTCA
     3601 ACGTGGTGGT GCGGGTATTT TCATGATTAT GGGTATCAGC GTTTGGGCCC AGGTCGGTGC
     3661 GCTCGTTGGC GTGATTCTGG TGACCCAGAA CAGCAAGCTG AAAAAAGATG CGATTCATAT
     3721 GTTGCCGGCC GGTTGCTTGG GTATTACCGA GCCGATTCTG TACGGTATTA GCCTGCCGAA
     3781 GAAACGTCCG CTGATCGCGG GCTGCATTGC AGCCTTTTTT GCTGGCGCTT ACTGCAACGC
     3841 GGTGGGCGTC ACCGCGCGCG CGGGTACGGG CTTCGGTATT TTCGAGTTTA TTGGCTTTTT
     3901 CTCGAGCCCT ACCATGGGCG GTACGGCGGA ACTGAGCAAC ATCAGCAATG GTCTGCTGTA
     3961 TATTAGCGGC GCGGCCATTG CGCTGGCGCT CGGTACCGTT TTCAGCCTGC TGATCTATAT
     4021 TGAGCGTCCG AACGAGAAAA GCAGCATTAG CAAGAGCGCA AATGCGTTGC TGAAATTCAT
     4081 CAAGGTGAAA GATAATCTGA CCGACGAAGA AATTCAAATT CTGAAGTCTC AAATTAAAGA
     4141 GATGAAAGCG GTTGTTAATA AAGACACCGT GAAGCAGATC AAGAAAATCG AGAAGCAAAT
     4201 CCAGAAGGTC GTTAGCGTCG ACAGCAAGAT TGAAACGCTG ACCGAGAATG AGTACAAACA
     4261 CGAGCAACAG ATCTATAAGA AAGGCAAGAA GGCCATTAGC AAGAATGATC TGCAGACTAG
     4321 CAAAAAGCTG GTGAACGAAT TTAACAGCCT GTCGTACAAG GCAAAGATTG ATAAGCTGAA
     4381 GATTGAGCGT GACGAACTGA AGGCAAAAAT TGATTTTAAG ACTCTCGACA AGATTATCGA
     4441 GCCTAAACAG AAAGAAATCG ACAATTTACT GAGCCAGATC TCCAAGCAAT ACAACTTAAA
     4501 AGAGGAAATT GTCGAAATCA AGAATAATTA CTGGAATGAC CTGAATTCGC TGAAGATTGC
     4561 GTACGACTTC GAAAATCCGA AGAACATCAA CATCTCCCTG AAACAGCTGA CTAAGAGCCT
     4621 GAAGAAAAAC AAAAAAGCGG TCAAGACCAT TTGAAGAGCT TAGAGACCGA GGATTCGGTC
     4681 AGTTCACT
//