LOCUS Exported 3248 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3248 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3214 /label="Mfl621" /note="This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis." /translation="MAKEAFDRSLPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERGITINTSHVEYKTENRHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSAYDFDGDGAPVIRGSALGALNGEAKWVAAIEELMAAVDEYIPTPTRDSDKTFLMPVEDVFTITGRGTVATGRVERGTIKVNEEVEIVGLVEEAKKTVVTGLEMFRKLLDFAEAGDNVGALLRGVDRESIERGQVLAKPGTIKPHTKLQASVYALTTEEGGRQKPFFNKYRPQFYFRTTDVTGEVILPAGTDMVMPGDNVEMTVELIKPIAVEDGTKFSIREGGRTIGAGTVISVQ" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCAAA TGGCTAAGGA 2041 GGCGTTTGAC CGTAGCTTAC CGCATGTGAA TATCGGTACT ATCGGTCACG TCGACCACGG 2101 TAAAACTACC CTGACTGCAG CGATTACCAA AGTCCTGGCG GACAAGGGTG GTGCGGAATT 2161 CAAGGACTAC GCCAACATCG ATAATGCGCC GGAGGAGCGT GAACGTGGTA TCACCATTAA 2221 TACCAGCCAC GTCGAGTACA AAACTGAAAA TCGTCACTAT GCTCACGTGG ATTGCCCGGG 2281 CCACGCGGAT TACGTTAAGA ACATGATCAC CGGTGCGGCG CAAATGGATG GTGGTATCCT 2341 GGTTGTCGCT GCCACCGATG GCCCGATGCC TCAAACGCGC GAGCATATCC TGCTGAGCCG 2401 CCAAGTGGGT GTGCCGAAGA TTGTCGTTTT TCTGAATAAG TGCGACATGG TTGACGATGA 2461 GGAAATGATC GACCTGGTTG AAATGGAGGT TCGCGATCTG CTGAGCGCTT ATGACTTTGA 2521 TGGCGACGGC GCGCCGGTGA TTCGTGGCTC GGCGTTGGGT GCGCTGAACG GCGAGGCGAA 2581 GTGGGTTGCT GCCATTGAGG AACTGATGGC AGCAGTTGAT GAATACATCC CGACTCCGAC 2641 GCGCGACAGC GACAAAACGT TCCTGATGCC GGTTGAGGAT GTCTTTACGA TCACTGGCCG 2701 CGGTACGGTG GCGACGGGTC GTGTGGAGCG TGGTACCATC AAAGTTAACG AGGAGGTCGA 2761 GATCGTGGGT CTGGTGGAGG AGGCCAAGAA AACGGTTGTG ACGGGTCTGG AAATGTTCCG 2821 TAAGCTGCTG GATTTCGCAG AAGCGGGCGA TAATGTGGGC GCATTGTTGC GTGGCGTGGA 2881 TCGTGAAAGC ATTGAACGCG GCCAGGTTCT GGCAAAACCG GGTACCATTA AACCGCATAC 2941 GAAACTGCAA GCGAGCGTGT ACGCACTGAC GACCGAGGAA GGCGGCCGTC AGAAACCGTT 3001 CTTCAATAAA TACCGTCCGC AGTTCTACTT CCGTACGACT GACGTTACCG GTGAAGTGAT 3061 TCTCCCGGCA GGCACCGATA TGGTGATGCC GGGCGATAAT GTTGAAATGA CTGTTGAGCT 3121 GATTAAGCCG ATCGCGGTCG AGGATGGCAC CAAGTTCTCT ATCAGAGAAG GTGGTCGCAC 3181 GATCGGCGCG GGCACCGTTA TTAGCGTGCA GTGAAGAGCT TAGAGACCGC GGGTTAGATA 3241 CTGCAACT //