LOCUS       Exported     4133 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4133
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4099
                     /note="Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome."
                     /translation="MPREFSLENTRNLGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMDWMAQEQERGITITSAATTAFWKNNRFNIIDTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETVWRQATTYRVPRIVFVNKMDKTGADFIYSVKSIGDRLGAKAAPIQLPIGAEDNFTGIIDLVEMKAYEFDGKAEEIAKEIEIPADLKDQAEILRSELVEAAVEYDEELMMKFLDGEEITIPELKQAIRKGVIGAEFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDVPSIKGVLPNGEEAERHADDNEPFSALAFKVMTDPFVGKLTFFRVYSGILTKGSYVLNSTKGDKERVGRILQMHANNRNEIEEVYAGDIAAAVGLKNTTTGDTLVDEKHEIILESMVFPEPVIQLALEPKTKADQEKMGLALSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRMRREFKVETNVGAPQVSYRETIKLPAKAEGKYVKQSGGRGSYGHVVIEFEPNVDKGFEWVDKITGGRVSKEYINAARVGLENALTNGVVAGYPMIDVKATIVDGSMHDVDSNEMAYKIAASFALKEACKKMNPVILEPIMNVEVTVPDEYYGDVMGNISSKRGLIEGSEQRGNAQTIKSKVPLTEMFGYATELRSFTQGRGNYTMIFSHYAEAPRSIAEEIIKKSGK"
                     /label="Mfl622"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAATAAGAC GACGACCCTC GGGTCTCAAA TGCCGCGCGA
     2041 GTTTAGCCTG GAGAATACCC GTAACCTGGG CATTATGGCA CATATTGACG CGGGTAAGAC
     2101 GACCACCACT GAGCGCATCT TGTTCCATAC CGGTAAAATC CATAAAATTG GTGAAACGCA
     2161 CGAGGGTGCG AGCCAGATGG ATTGGATGGC CCAGGAGCAG GAGCGTGGCA TTACGATTAC
     2221 CAGCGCTGCG ACCACGGCGT TTTGGAAGAA TAATCGTTTT AATATTATCG ATACCCCAGG
     2281 TCACGTTGAT TTCACGGTGG AGGTGGAACG TAGCCTGCGC GTTCTGGACG GCGCAGTGGC
     2341 GGTGCTGGAC GGCCAGAGCG GTGTGGAGCC TCAGACCGAA ACGGTGTGGC GCCAAGCGAC
     2401 CACCTACCGT GTTCCACGCA TCGTTTTTGT TAATAAGATG GATAAGACCG GCGCCGATTT
     2461 CATCTACAGC GTGAAAAGCA TCGGCGATCG TCTGGGTGCT AAGGCAGCGC CGATCCAATT
     2521 GCCGATCGGC GCTGAAGATA ACTTCACCGG TATCATTGAC CTCGTCGAGA TGAAGGCGTA
     2581 CGAATTTGAC GGTAAAGCGG AAGAGATCGC CAAGGAAATT GAAATTCCAG CGGACCTGAA
     2641 AGACCAAGCA GAGATCCTCC GTAGCGAACT GGTTGAGGCT GCAGTGGAAT ATGACGAGGA
     2701 GCTGATGATG AAATTCCTGG ATGGTGAAGA GATTACCATC CCTGAGTTGA AGCAGGCTAT
     2761 CCGCAAAGGT GTCATTGGTG CAGAGTTCTT TCCGGTGCTG GCAGGCAGCG CATTCAAGAA
     2821 TAAAGGTGTT AAGCTGCTGT TGGATGCCGT GGTTGACTAT CTGCCGAGCC CGCTGGACGT
     2881 TCCGTCTATT AAAGGTGTCT TGCCGAACGG TGAGGAAGCG GAGCGTCACG CAGATGACAA
     2941 CGAGCCGTTC AGCGCCCTGG CTTTTAAAGT GATGACCGAT CCTTTCGTGG GCAAGCTCAC
     3001 TTTCTTTCGT GTGTATAGCG GCATTTTGAC GAAAGGTAGC TATGTTCTGA ACAGCACCAA
     3061 GGGCGACAAA GAACGTGTCG GCCGTATTCT GCAAATGCAC GCGAATAATC GTAATGAGAT
     3121 CGAAGAGGTC TATGCCGGTG ACATTGCGGC AGCGGTGGGT CTGAAAAATA CGACCACCGG
     3181 TGACACCCTG GTCGATGAGA AGCACGAAAT CATCCTGGAG AGCATGGTTT TCCCAGAGCC
     3241 GGTCATCCAA CTGGCCCTGG AACCTAAGAC GAAAGCCGAC CAAGAGAAAA TGGGCTTGGC
     3301 ACTCAGCAAG TTGGCTGAAG AGGACCCGAC GTTCCGTACC TATACCGATG AGGAAACCGG
     3361 CCAGACCATT ATCGCGGGTA TGGGTGAGCT GCATCTGGAC ATTATTGTTG ACCGCATGCG
     3421 TCGTGAGTTC AAAGTCGAAA CGAACGTCGG TGCCCCTCAG GTTAGCTATC GTGAAACTAT
     3481 CAAACTGCCT GCTAAGGCGG AGGGTAAGTA TGTTAAGCAG AGCGGTGGTC GTGGTAGCTA
     3541 TGGCCACGTG GTCATTGAAT TCGAGCCGAA CGTCGATAAG GGCTTTGAGT GGGTCGACAA
     3601 GATTACGGGT GGTCGTGTCT CTAAGGAGTA TATTAATGCT GCGCGCGTGG GTTTAGAGAA
     3661 CGCTCTGACC AACGGCGTTG TGGCGGGTTA TCCGATGATC GATGTCAAAG CAACTATCGT
     3721 TGATGGTAGC ATGCACGACG TCGACTCCAA TGAGATGGCA TATAAGATCG CCGCGTCTTT
     3781 CGCGCTGAAG GAGGCATGTA AGAAAATGAA CCCGGTGATC TTGGAGCCGA TTATGAATGT
     3841 GGAAGTTACG GTGCCGGACG AGTACTATGG CGACGTCATG GGCAACATTA GCTCTAAGCG
     3901 CGGTTTGATT GAAGGCAGCG AACAGCGTGG CAATGCGCAG ACGATTAAGT CCAAGGTTCC
     3961 TTTGACGGAA ATGTTTGGTT ATGCAACGGA GCTGCGTAGC TTCACCCAAG GTCGTGGCAA
     4021 CTATACGATG ATCTTTAGCC ATTACGCTGA AGCGCCGCGT AGCATTGCGG AGGAAATCAT
     4081 TAAAAAGAGC GGTAAGTGAA GAGCTTAGAG ACCGCTCGAT GCCATAGTTC CTT
//