LOCUS       Exported     3629 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3629
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3595
                     /label="Mfl626"
                     /note="Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol."
                     /translation="MIMKKPFVATASLIAMFSIGGALYVAINLCLNFFIPTPLPFIFFISATHIVSMVWAIVVLCNRKRRIETRIRWAIFIIVIPFFGIMSYIFLGRVYKYNKNKNYLYNKNSVSVLQPKVQYDIENLKEIERLNPEFKRSFMMTFEQQREGIYANTEIEYLKSGNEYFSNLLNDIKNAKEYVMINCYIISEGEFLSKLTSLLVEKMSEGVRVYIIYDFLGSYGRFTTSKKRLIQEGANLIAYSPIHFPFLKWNANYRDHRKDISIDGQIGYLGGVNISDEYINKSGVFGFWNDSAIRINGEGVQEIEAIFQKDWNFYVTKKQKKIEKLEPKFGKAIRKRYTTDEFIQIVSDGPNHDRPICLELLLNLIHSAQNRIWLKSPYFIPPPEIINALCNAASTGLDVRILLPGRSDKFLLLEVSKQWTKKMFENGVKIYSMNDTFIHEKAYIFDEAISFTGSSNLDYRALFCDQQTMALIRSNNMNKKIEEKMIHDMDKSFEYKFMPNKDLPLWKRAIVKVYNIMAPLL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCGCTC GGATAATACT CGGTCTCAAA TGATCATGAA
     2041 AAAGCCATTC GTGGCGACCG CCTCCCTGAT CGCAATGTTT AGCATTGGTG GCGCGCTGTA
     2101 CGTTGCGATT AACCTGTGCC TTAACTTTTT TATTCCGACC CCGCTGCCGT TTATCTTCTT
     2161 TATCAGCGCG ACGCACATTG TCTCCATGGT GTGGGCCATT GTCGTCCTGT GTAACCGCAA
     2221 GCGTCGTATC GAAACCCGTA TCCGCTGGGC AATCTTTATT ATTGTGATCC CGTTCTTTGG
     2281 TATTATGTCG TATATCTTTC TGGGCCGCGT GTATAAATAC AATAAGAATA AAAACTACTT
     2341 GTACAATAAA AACAGCGTCA GCGTGTTGCA ACCGAAAGTC CAGTATGATA TTGAAAACCT
     2401 GAAAGAAATC GAGCGTCTGA ACCCAGAATT CAAACGTTCG TTCATGATGA CGTTCGAGCA
     2461 ACAGCGCGAG GGCATCTATG CAAACACCGA AATCGAGTAT TTAAAGAGCG GCAACGAGTA
     2521 CTTCAGCAAT CTGCTGAATG ATATCAAGAA TGCAAAAGAG TATGTCATGA TCAATTGCTA
     2581 TATTATTTCT GAGGGTGAAT TCCTGAGCAA ACTGACCTCC CTGCTGGTTG AGAAGATGAG
     2641 CGAAGGTGTT AGAGTGTATA TTATCTACGA CTTTCTGGGT TCCTACGGTC GTTTTACCAC
     2701 CAGCAAAAAA CGTCTGATTC AAGAAGGTGC CAATCTGATT GCCTACTCCC CGATTCACTT
     2761 CCCGTTCTTG AAATGGAACG CAAACTATCG TGACCATCGT AAAGATATTT CCATCGACGG
     2821 TCAAATCGGC TATTTAGGCG GTGTGAATAT TAGCGACGAG TACATCAATA AAAGCGGTGT
     2881 TTTTGGTTTC TGGAACGACA GCGCGATCCG CATCAATGGT GAGGGCGTTC AGGAAATCGA
     2941 GGCGATTTTT CAGAAAGATT GGAATTTTTA CGTCACCAAG AAACAGAAGA AAATTGAGAA
     3001 ACTGGAACCA AAATTCGGTA AGGCGATTCG TAAACGCTAC ACCACTGATG AGTTTATTCA
     3061 GATTGTTTCT GACGGTCCGA ACCACGATCG TCCGATCTGC CTGGAGCTCC TGCTCAATTT
     3121 GATTCATAGC GCTCAAAACC GCATCTGGTT AAAGAGCCCG TATTTTATCC CGCCGCCGGA
     3181 AATTATTAAC GCGCTGTGTA ATGCGGCAAG CACCGGCCTG GATGTGCGTA TCCTGCTGCC
     3241 GGGTCGTAGC GACAAGTTCT TGCTCCTGGA GGTCAGCAAA CAGTGGACCA AGAAGATGTT
     3301 CGAGAATGGT GTTAAAATCT ACAGCATGAA CGATACCTTC ATTCACGAAA AAGCATACAT
     3361 CTTTGATGAA GCGATTAGCT TCACCGGCAG CAGCAATCTG GACTACCGCG CTCTGTTTTG
     3421 TGATCAGCAA ACCATGGCAC TGATCCGTTC TAACAACATG AATAAGAAAA TTGAAGAAAA
     3481 GATGATCCAC GATATGGACA AGAGCTTCGA GTATAAGTTT ATGCCGAATA AAGATCTGCC
     3541 GCTGTGGAAA CGTGCAATCG TCAAGGTCTA CAATATTATG GCGCCGCTGC TGTGAAGAGC
     3601 TTAGAGACCC CGATAGTTCC AGCTAGCAT
//