LOCUS       Exported     2977 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2977
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1015..1062
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2910..2926
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2927..2958
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..943
                     /label="Mfl628"
                     /note=""
                     /translation="MRDTYDIVFDHKDNKHFNQDLSNLIKRSSNNNFEKWLFKDKQLENFSPITIVNEKNEIIAALGILKFDVFISGEKCSAVQVGNFFIKEAFIEEKLEEILIEAAIYKYSNLVDILISYCGVKDEEVLIKSGFERKQEYLFFLPWNNREKQESSKIIKLDLSLAHDFELVKEAILHSSKQSSILDTKGDSLVKFHNIQKHFEDDLWYIPSLQTVVCFRIKNGIFDFIGSFSSHNIDLEELLKIIVPNGIKRVDFGFMPDIKEGIHITKTDTFVKTGLDIHSCLYLKEVTTGLKDKKFMFPLLSRQK"
     primer_bind     complement(1067..1083)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1150..1738)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1929..2789)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2790..2894)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATAGGAACC TCTTACGCGG GGTCTCAAAT GAGAGACACC TATGATATTG TTTTCGACCA
       61 TAAGGACAAT AAGCACTTCA ACCAGGACCT TTCCAATTTG ATTAAGCGTT CCAGCAACAA
      121 CAATTTCGAA AAATGGCTGT TCAAAGACAA GCAGCTGGAA AATTTTTCAC CGATCACGAT
      181 CGTGAACGAG AAAAACGAGA TTATCGCCGC CCTGGGCATT CTGAAGTTTG ACGTTTTCAT
      241 CAGCGGCGAA AAATGCAGCG CGGTTCAGGT CGGTAACTTC TTTATCAAAG AGGCATTCAT
      301 CGAGGAAAAG CTGGAAGAAA TCCTGATCGA AGCGGCCATT TACAAATATA GCAATTTGGT
      361 GGACATCCTG ATTTCGTATT GTGGTGTGAA AGACGAGGAG GTTCTGATTA AATCCGGCTT
      421 TGAGCGTAAG CAGGAGTACC TGTTTTTTCT GCCGTGGAAC AATCGCGAAA AGCAAGAAAG
      481 CAGCAAGATT ATCAAACTGG ACCTGAGCCT GGCGCACGAT TTCGAGCTGG TTAAGGAGGC
      541 GATCTTGCAT AGCAGCAAGC AGAGCAGCAT TCTGGATACG AAGGGTGACA GCCTGGTCAA
      601 ATTCCATAAC ATCCAGAAAC ACTTTGAAGA TGATCTGTGG TACATCCCAA GCCTCCAAAC
      661 CGTGGTTTGC TTCCGTATCA AAAATGGTAT TTTCGATTTC ATTGGCTCGT TTTCGAGCCA
      721 CAATATTGAT CTGGAGGAAC TGCTGAAAAT CATCGTGCCG AACGGTATTA AGCGTGTGGA
      781 TTTTGGCTTC ATGCCGGACA TCAAGGAGGG TATTCACATT ACTAAGACGG ACACCTTCGT
      841 CAAAACGGGC CTGGATATTC ATAGCTGTCT GTACTTGAAG GAGGTTACCA CGGGTCTGAA
      901 AGATAAGAAA TTTATGTTTC CGTTGCTGAG CCGTCAGAAG TGAAGAGCTT AGAGACCGTA
      961 GTGCCTGTTC GTTCTGTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1021 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1081 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1141 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1201 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1261 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1321 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1381 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1441 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1501 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1561 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1621 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1681 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1741 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1801 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1861 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1921 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     1981 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2041 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2101 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2161 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2221 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2281 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2341 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2401 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2461 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2521 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2581 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2641 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2701 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2761 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2821 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2881 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     2941 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//