LOCUS       Exported     3154 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3154
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1192..1239
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3087..3103
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3104..3135
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1120
                     /label="prfA"
                     /note="Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA."
                     /translation="MNPKTYEALETMQKRVDQIDKDLQSEEIVSDVKKMLELNKERANLIEVVEKFIEYKTIIQSIADAKEILGNEKDAEMIELAKMELSENEDAVEPIVAIIEELLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQNWKITMLDANSSEAGGFSQVSFMVKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSDVEIEIKNSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGVVAASQDGRSQHDNKDIAMTMLRARIYEAELEKQQAEADATRKNAVGTGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGKIDDFIIALVNDEQRQKVEAQLQDNE"
     primer_bind     complement(1244..1260)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1327..1915)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2106..2966)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2967..3071)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GAATCCAAAG ACCTACGAGG CCCTGGAAAC
       61 GATGCAGAAG CGTGTGGACC AGATCGATAA GGATCTGCAG TCTGAGGAAA TCGTGAGCGA
      121 CGTGAAGAAA ATGCTGGAGC TCAATAAAGA GCGTGCGAAC CTGATTGAGG TTGTAGAAAA
      181 GTTCATTGAG TACAAGACCA TTATCCAATC TATCGCGGAC GCTAAGGAGA TTCTGGGCAA
      241 TGAGAAAGAT GCGGAAATGA TCGAACTGGC CAAGATGGAG CTCTCTGAAA ATGAAGATGC
      301 CGTCGAACCG ATTGTTGCGA TTATCGAAGA ACTGCTGCTG CCGAAAGACC CGAATGACGA
      361 TAAGAACGTT ATCGTTGAAA TCCGCGGTGC AGCGGGTGGC GACGAAGCGA ACATCTTTGC
      421 GGGTGATCTG CTGAGAATGT ATAAACTGTA TGCGGAAACC CAAAACTGGA AAATCACGAT
      481 GCTGGATGCC AACTCTTCCG AAGCCGGCGG TTTTAGCCAA GTGAGCTTCA TGGTCAAAGG
      541 CGACCGCGTC TACAGCAAAC TGAAGTTCGA GTCCGGTGCT CACCGTGTTC AAAGAGTCCC
      601 GAAAACCGAA GCGAAAGGCC GCATCCAGAC CAGCACCGCT ACCGTGGCAG TACTCCCGGA
      661 GATGTCTGAT GTCGAAATTG AGATCAAAAA CAGCGATCTG CGTATCGACA CCTATCGCAG
      721 CAGCGGCGCG GGTGGTCAAC ATGTTAATAC CACCGATAGC GCGGTCCGTA TTACGCACAT
      781 TCCGACCGGC GTGGTGGCTG CTAGCCAGGA TGGTCGTAGC CAACATGACA ACAAGGATAT
      841 CGCTATGACC ATGCTGCGTG CGCGTATCTA TGAAGCAGAA CTGGAGAAGC AACAGGCAGA
      901 AGCCGACGCC ACCCGTAAAA ACGCTGTAGG CACCGGCGCG CGTAGCGAAA AGATCCGTAC
      961 CTATAACTAC CCGCAAAATC GTGTGACGGA TCACCGCGTC GGTCTGACGC TGAATAAGTT
     1021 AGACCAAGTC ATGGAAGGTA AGATTGACGA TTTCATCATT GCGCTGGTTA ACGACGAGCA
     1081 GCGCCAAAAG GTTGAAGCAC AACTGCAGGA TAATGAGTGA AGAGCTTAGA GACCCGCTAA
     1141 GATCCGTAGG TCTGCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1201 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1261 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1321 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1381 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1441 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1501 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1561 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1621 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1681 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1741 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1801 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1861 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG
     1921 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     1981 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     2041 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     2101 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     2161 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     2221 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     2281 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     2341 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     2401 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     2461 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     2521 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     2581 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     2641 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     2701 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     2761 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     2821 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     2881 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     2941 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     3001 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     3061 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC
     3121 CGGAGGCTTT TGACTTGGTC AGGTGGAGTG GGAG
//