LOCUS       Exported     3940 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3940
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1978..2025
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3873..3889
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3890..3921
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1906
                     /label="Mfl637"
                     /note=""
                     /translation="MSYKIKNFSPSNNKETLKKVDFESKPGQITNVYLEDEKQKSFKKIILGRQKNKTGRYQVDNEDVINKNFIKLHTEYIKTDTWFERLVPTKWILALSLLSDLQFLRNARIKYAEKKYEYLSLLDSKNDLSDLKLRRDIDEAINKFIKFSNESHEKIINNLDDVLSKLNEGMSQKMFNEFSPQVKVLSKALFDIEKEKLLKRMEFKFVQVLYDRVESLNELRDCCTCEFNTKKSNNKLLRKRKNLFEYKQTKFIVEKQLKFLNIRLNKLRLESILLFFRKRTISTQFKFELDKFYKTYSVAKEVQQEISSELNKWKKVIKDKVSDQNLTEKKFLFNFIKTESSLLTKFIISNIHNYHKLILTGEVKYGNKNEFLSLRKHYKKQIVPVFKQAELWMENNFKNLDINFEWFLKGSFKLTSLNMIYVKILKAINLGKKNIILSDFLHLLTNKDYEMLFDTISKINEFYPEISFIILHSRTFTISDISKNKKIYWSRNNELVKTTNKEFFEKNLIDICKNELGESNVFSYTKESDNLIDVENRLWNVNTKKFNESGFIFINPLEISTEFIENNSVPINVRIIRTNKYHDKNMYFGITKNKTKIYFYSEDKTFERENEAVLYFEEHSISAII"
     primer_bind     complement(2030..2046)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2113..2701)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2892..3752)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3753..3857)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GTCCTACAAA ATCAAGAATT TTAGCCCGAG
       61 CAATAATAAG GAAACTCTGA AGAAAGTTGA CTTCGAAAGC AAACCAGGCC AGATCACCAA
      121 CGTGTATCTG GAGGACGAAA AACAAAAGAG CTTTAAGAAG ATCATTTTGG GTCGTCAGAA
      181 AAATAAAACG GGTAGATACC AGGTTGACAA TGAGGATGTG ATTAACAAGA ACTTCATCAA
      241 ATTGCACACG GAGTATATCA AAACCGATAC CTGGTTTGAA CGCTTGGTTC CGACCAAATG
      301 GATCCTGGCC CTGAGCCTGC TGAGCGATCT GCAGTTTCTG CGTAATGCCC GTATCAAATA
      361 CGCAGAGAAA AAATACGAGT ACTTGAGCCT TCTGGATAGC AAGAACGATC TGAGCGACCT
      421 GAAGCTGCGT CGCGATATCG ACGAAGCAAT TAACAAGTTC ATTAAGTTCT CCAATGAAAG
      481 CCACGAGAAA ATCATCAACA ACCTGGATGA TGTGCTGTCC AAACTGAACG AAGGTATGAG
      541 CCAGAAAATG TTTAATGAAT TCAGCCCACA GGTTAAAGTA TTGAGCAAAG CGCTGTTCGA
      601 CATTGAGAAG GAGAAACTGC TGAAGCGTAT GGAGTTCAAA TTCGTCCAGG TCCTGTACGA
      661 TCGCGTGGAG AGCCTGAACG AACTGCGCGA CTGTTGTACG TGCGAATTCA ACACCAAGAA
      721 ATCCAATAAT AAACTGCTGC GTAAACGTAA AAATCTGTTC GAATATAAGC AGACCAAGTT
      781 TATCGTGGAG AAGCAACTGA AGTTTCTCAA CATCCGCCTG AACAAGCTGC GCCTGGAATC
      841 CATCCTTCTG TTTTTTCGTA AGCGTACCAT TAGCACTCAG TTTAAGTTCG AGTTGGATAA
      901 GTTCTACAAG ACCTATTCTG TCGCGAAAGA AGTGCAACAG GAGATTTCGA GCGAGCTGAA
      961 CAAATGGAAA AAAGTGATCA AAGATAAGGT GAGCGACCAG AATTTGACCG AAAAGAAATT
     1021 TCTGTTTAAC TTCATTAAGA CTGAGAGCAG CCTGCTCACC AAATTTATTA TCAGCAACAT
     1081 TCACAACTAT CACAAGCTGA TCCTGACCGG TGAAGTTAAA TACGGTAACA AGAATGAATT
     1141 TTTGTCCCTG CGTAAGCATT ATAAGAAGCA AATCGTGCCG GTTTTCAAGC AAGCGGAGCT
     1201 GTGGATGGAG AATAATTTCA AGAATCTGGA CATCAATTTT GAGTGGTTTC TGAAAGGTAG
     1261 CTTTAAACTG ACGAGCCTCA ATATGATTTA TGTTAAAATC CTGAAAGCAA TTAATCTGGG
     1321 TAAGAAAAAC ATCATCCTGT CGGACTTCCT GCACTTGCTG ACCAACAAGG ATTACGAGAT
     1381 GCTTTTTGAC ACGATTAGCA AGATTAATGA GTTCTACCCA GAAATCAGCT TCATCATCCT
     1441 GCATTCGCGC ACCTTTACGA TCTCCGATAT TAGCAAAAAC AAAAAGATTT ATTGGTCACG
     1501 CAACAACGAA CTGGTCAAGA CGACCAATAA GGAGTTCTTC GAGAAAAACC TGATTGATAT
     1561 TTGTAAGAAT GAGCTTGGCG AGAGCAATGT GTTCTCGTAC ACCAAAGAGT CCGATAACCT
     1621 GATCGACGTT GAAAATCGTT TGTGGAATGT CAACACCAAG AAATTCAACG AGAGCGGTTT
     1681 CATTTTCATC AACCCTCTGG AGATCAGCAC CGAATTCATT GAAAACAATT CTGTTCCGAT
     1741 TAATGTTCGC ATCATCCGTA CCAACAAATA TCACGATAAG AATATGTACT TCGGTATCAC
     1801 CAAAAATAAA ACCAAAATCT ATTTCTACTC CGAGGACAAG ACCTTCGAGC GTGAGAACGA
     1861 GGCTGTCCTG TACTTCGAGG AGCATAGCAT CAGCGCTATC ATTTGAAGAG CTTAGAGACC
     1921 GGTCTTGTGC GTGACTATCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1981 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     2041 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     2101 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     2161 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2221 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2281 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2341 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2401 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2461 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2521 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2581 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2641 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2701 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2761 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2821 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2881 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2941 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     3001 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     3061 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     3121 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     3181 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     3241 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     3301 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     3361 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     3421 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     3481 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3541 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3601 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3661 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3721 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3781 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3841 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3901 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//