LOCUS       Exported     2878 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2878
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      916..963
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2811..2827
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2828..2859
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..844
                     /label="Mfl641"
                     /note=""
                     /translation="MSKNLTFAEMSALDQINKNINFFVSNNLQNLSKNYNVGEATLVRLVKKRGFSSLKEMQISYAKKIDIENLFGNSDNNQNTKIINDVANFEVFSIIQTSECINIKDINKTAELLINSNKRLFFGIENSQLSATFLANNLQKIGLNCSVYQSVHGAITELNFLNKEDSIILIFSSSGETKECIEVAKIAHEMNLRYIWIGSEFASKKSEYLQMANIKIFHSYHKNEILRFPNISSSSGQLFISNLLFNIVINNKDGTSDILTKSNTMVKGWNS"
     primer_bind     complement(968..984)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1051..1639)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1830..2690)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2691..2795)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GAGCAAGAAT TTAACGTTCG CGGAGATGAG
       61 CGCCCTGGAT CAGATCAACA AGAACATTAA CTTTTTTGTT TCCAATAATC TGCAAAACCT
      121 GTCCAAGAAC TACAATGTCG GTGAGGCGAC TCTGGTCCGT CTGGTTAAAA AGCGTGGTTT
      181 TAGCAGCCTG AAAGAGATGC AGATTAGCTA CGCGAAGAAA ATCGATATTG AAAATCTGTT
      241 TGGCAATAGC GACAATAATC AGAATACCAA GATTATTAAT GATGTCGCAA ATTTTGAGGT
      301 TTTCAGCATT ATCCAAACGA GCGAGTGCAT CAATATTAAA GATATCAACA AAACGGCCGA
      361 ACTGCTGATT AACAGCAATA AGCGCCTGTT TTTCGGTATC GAGAACTCCC AGTTGTCTGC
      421 AACCTTCCTG GCCAATAATC TGCAAAAAAT CGGTCTGAAT TGCAGCGTGT ATCAGAGCGT
      481 TCATGGTGCC ATTACCGAAC TGAACTTTCT GAACAAGGAG GATAGCATCA TTCTGATTTT
      541 CAGCAGCAGC GGTGAAACCA AGGAGTGCAT CGAGGTTGCC AAAATCGCAC ATGAGATGAA
      601 TCTGCGTTAC ATCTGGATCG GTAGCGAGTT CGCATCCAAA AAGTCCGAGT ATCTGCAGAT
      661 GGCTAATATC AAGATCTTCC ACAGCTACCA CAAGAACGAG ATTCTGCGTT TCCCGAACAT
      721 TAGCAGCAGC AGCGGCCAAC TCTTCATTAG CAATCTGCTG TTCAACATCG TGATCAACAA
      781 CAAGGATGGT ACGAGCGACA TTTTGACCAA AAGCAACACC ATGGTTAAAG GTTGGAATTC
      841 CTGAAGAGCT TAGAGACCGC CCGGCAATTC TTATAGCTCG CTGAGGTGTC AATCGTCGGA
      901 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      961 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1021 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1081 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1141 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1201 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1261 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1321 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1381 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1441 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1501 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1561 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1621 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1681 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1741 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1801 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1861 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1921 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     1981 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2041 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2101 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2161 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2221 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2281 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2341 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2401 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2461 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2521 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2581 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2641 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2701 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2761 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2821 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//