LOCUS       Exported     3572 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3572
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3538
                     /label="ulaA"
                     /note=""
                     /translation="MNEFIFFIKGFFGAPAVLIGLFALFGSLLQRKKFTEVLVSTLKAAVGYLILSGGIGLLVSTLDDFTTAFNELFGLQGVMPNSDALAGAIMEAIPEIATIAALMMFISIVLNIIFAKFSRFKYIFLTGHHTLFICVAVSTIFHFSGMSLSTDVWYFLIVGSAIVSLYMLLSPALTKKYMNHLTGDDKLYIGHANVMGFAIAGGIGGLVGKLKKGNVKSTEEIKFPKWLSVFSNSTVSVAVMMLMLFGVTYASLWGVAGKEQMVALGIIGENDSILVKIILDALIFTAGVEILIFGITTMINELIPALEGISKKLIPGAKMAVDCSVALSYSPTAVLIGFVSSFTGGIIGLLISIGLNLAAPAAIPAVIIPGIIAHFFTGGVSGTFGNIKGGILGAILGAFVNGIILTIVPLMFILMQSQLGWIFDSNGVLQWAESDYLIFNILGWILNAEWTKWLIMAIVFVGIGGLLVDGHFWIKKDKIKRPELYVKQKTTKKSTIKEEKSN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATATCACG CAAAAGCACC GGGTCTCAAA TGAATGAATT
     2041 TATCTTTTTC ATCAAAGGCT TTTTTGGTGC GCCTGCGGTG CTGATCGGTC TGTTTGCATT
     2101 GTTCGGTAGC CTGCTGCAAC GTAAGAAGTT CACGGAAGTT CTGGTGTCCA CGCTGAAAGC
     2161 GGCCGTTGGT TACCTGATTC TGAGCGGTGG TATTGGCCTG CTGGTCAGCA CCTTAGATGA
     2221 CTTCACCACC GCGTTTAACG AGCTGTTCGG TCTGCAGGGC GTGATGCCAA ATTCCGATGC
     2281 CCTGGCCGGC GCGATCATGG AGGCCATTCC GGAGATCGCA ACGATTGCCG CGTTGATGAT
     2341 GTTTATCAGC ATCGTGCTGA ATATTATTTT CGCGAAATTT AGCCGCTTTA AATATATTTT
     2401 CCTGACCGGC CACCACACTC TGTTTATCTG CGTTGCTGTA AGCACCATCT TCCACTTCTC
     2461 TGGTATGAGC CTGAGCACCG ATGTGTGGTA CTTCTTAATT GTCGGTAGCG CGATTGTTAG
     2521 CTTATATATG CTTCTGAGCC CGGCGCTGAC CAAAAAGTAC ATGAACCACC TTACGGGTGA
     2581 CGATAAGCTG TACATTGGTC ACGCGAATGT GATGGGTTTC GCGATCGCCG GCGGCATTGG
     2641 CGGTTTGGTT GGTAAACTGA AGAAAGGCAA TGTAAAGAGC ACTGAAGAAA TCAAATTCCC
     2701 GAAGTGGCTG AGCGTGTTCA GCAACAGCAC CGTCAGCGTT GCGGTCATGA TGTTGATGCT
     2761 GTTCGGCGTG ACGTACGCTT CTCTGTGGGG TGTGGCTGGT AAAGAACAGA TGGTAGCTCT
     2821 GGGTATCATC GGTGAAAACG ATTCTATTCT GGTTAAGATC ATTCTGGACG CACTGATCTT
     2881 CACCGCTGGC GTTGAAATCC TGATCTTCGG TATCACCACC ATGATTAATG AACTCATCCC
     2941 GGCACTGGAG GGCATTTCCA AGAAGTTAAT TCCGGGCGCC AAAATGGCGG TGGATTGTTC
     3001 TGTTGCTCTG AGCTATAGCC CAACGGCCGT GCTGATCGGC TTCGTGAGCA GCTTCACCGG
     3061 TGGTATCATC GGTCTGCTGA TTAGCATTGG CCTGAATCTG GCGGCGCCGG CCGCGATTCC
     3121 AGCGGTTATT ATTCCGGGTA TTATTGCTCA CTTTTTTACC GGCGGCGTCA GCGGTACCTT
     3181 TGGCAACATT AAGGGCGGTA TTTTGGGTGC GATCTTGGGT GCATTCGTTA ACGGCATTAT
     3241 CCTGACTATT GTGCCGCTGA TGTTCATCCT GATGCAGTCT CAGTTGGGTT GGATTTTTGA
     3301 CTCCAACGGC GTGCTGCAAT GGGCAGAGTC CGACTACTTG ATCTTCAACA TTCTGGGTTG
     3361 GATCCTGAAT GCGGAATGGA CGAAGTGGCT GATTATGGCA ATTGTGTTTG TGGGTATTGG
     3421 TGGTCTGCTG GTCGATGGTC ATTTTTGGAT TAAAAAAGAC AAGATTAAAC GCCCTGAACT
     3481 GTACGTGAAG CAGAAAACCA CCAAAAAGAG CACGATCAAG GAGGAGAAGT CTAACTGAAG
     3541 AGCTTAGAGA CCCGAGTGTA GTTCCCGTCA AT
//