LOCUS       Exported     2953 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2953
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      991..1038
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2886..2902
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2903..2934
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..919
                     /label="Mfl644"
                     /note=""
                     /translation="MTRLYHEKLVNATAMVKAAHENKYAIGHFNINNLEWTKAILEAAQASNTPVILGTSEGAIKYMGGPVTVVGMVNGLLEELNITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEENLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGELGDPTQAAEISKTGIAMLAAGIGNIHGKYPEWWTGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTELQLAFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTEWFGCKGKA"
     primer_bind     complement(1043..1059)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1126..1714)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1905..2765)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2766..2870)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GACCCGCCTG TACCATGAGA AGCTGGTGAA
       61 TGCCACCGCC ATGGTGAAGG CGGCACATGA AAACAAATAC GCCATCGGCC ACTTCAATAT
      121 CAATAATTTG GAGTGGACCA AGGCCATTCT GGAGGCAGCC CAAGCGTCCA ATACCCCGGT
      181 TATCCTGGGT ACTAGCGAAG GCGCGATCAA ATACATGGGT GGTCCTGTGA CGGTTGTGGG
      241 TATGGTCAAT GGCCTGTTGG AAGAATTGAA TATTACGGTC CCGGTGGCTT TGCATCTGGA
      301 TCACGGTCAG TCCATCGAGA TGGCGAAGAA ATGTATTTTA GCGGGTTACA GCAGCGTCAT
      361 GTTTGATGGT AGCCACCTCC CATACGAGGA GAACCTGGCT AAGACGAAAG AACTGCTGAT
      421 TTTTGCGAAT GAGCACGAAA TTAGCGTTGA GGCGGAGATT GGTTCGATTG GCGGTGAAGA
      481 GGACGGTGTT GTTGGTAACG GTGAGCTGGG CGATCCAACC CAAGCGGCGG AGATTAGCAA
      541 GACCGGTATT GCTATGCTGG CGGCGGGTAT CGGTAACATC CATGGTAAGT ACCCGGAATG
      601 GTGGACCGGC CTGAGCTTCG ACACCCTGGA AGAACTGCAA GCAGCGTGTA AAATGCCGAT
      661 GGTGCTGCAC GGTGGCTCCG GTATCCCGCA GGACCAGGTC GAAAAGGCCA TCAGCATGGG
      721 TATTAGCAAG ATTAACGTGA ACACCGAGCT GCAACTGGCA TTCCGTGATG CTACGCGTAA
      781 GTACATTGAA GCCGGCAAAG ATCTGGACGA CAAGACGAAA GGTTTCGATC CGCGCAAACT
      841 GTTGAATCCG GGCTACCAGG CTCTGAAGGA AACGTTTAAT GAACTGACCG AGTGGTTCGG
      901 CTGTAAAGGT AAAGCCTGAA GAGCTTAGAG ACCCCGCGTG GTTCTTACAG TATCGCTGAG
      961 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1021 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1081 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1141 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1201 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1261 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1321 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1381 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1441 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1501 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1561 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1621 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1681 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     1741 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     1801 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     1861 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     1921 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     1981 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2041 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2101 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2161 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2221 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2281 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2341 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2401 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2461 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2521 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2581 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2641 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     2701 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     2761 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     2821 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     2881 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     2941 GGTGGAGTGG GAG
//