LOCUS Exported 2953 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2953 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 991..1038 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2886..2902 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2903..2934 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..919 /label="Mfl644" /note="" /translation="MTRLYHEKLVNATAMVKAAHENKYAIGHFNINNLEWTKAILEAAQASNTPVILGTSEGAIKYMGGPVTVVGMVNGLLEELNITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEENLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGELGDPTQAAEISKTGIAMLAAGIGNIHGKYPEWWTGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTELQLAFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTEWFGCKGKA" primer_bind complement(1043..1059) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1126..1714) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1905..2765) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2766..2870) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GACCCGCCTG TACCATGAGA AGCTGGTGAA 61 TGCCACCGCC ATGGTGAAGG CGGCACATGA AAACAAATAC GCCATCGGCC ACTTCAATAT 121 CAATAATTTG GAGTGGACCA AGGCCATTCT GGAGGCAGCC CAAGCGTCCA ATACCCCGGT 181 TATCCTGGGT ACTAGCGAAG GCGCGATCAA ATACATGGGT GGTCCTGTGA CGGTTGTGGG 241 TATGGTCAAT GGCCTGTTGG AAGAATTGAA TATTACGGTC CCGGTGGCTT TGCATCTGGA 301 TCACGGTCAG TCCATCGAGA TGGCGAAGAA ATGTATTTTA GCGGGTTACA GCAGCGTCAT 361 GTTTGATGGT AGCCACCTCC CATACGAGGA GAACCTGGCT AAGACGAAAG AACTGCTGAT 421 TTTTGCGAAT GAGCACGAAA TTAGCGTTGA GGCGGAGATT GGTTCGATTG GCGGTGAAGA 481 GGACGGTGTT GTTGGTAACG GTGAGCTGGG CGATCCAACC CAAGCGGCGG AGATTAGCAA 541 GACCGGTATT GCTATGCTGG CGGCGGGTAT CGGTAACATC CATGGTAAGT ACCCGGAATG 601 GTGGACCGGC CTGAGCTTCG ACACCCTGGA AGAACTGCAA GCAGCGTGTA AAATGCCGAT 661 GGTGCTGCAC GGTGGCTCCG GTATCCCGCA GGACCAGGTC GAAAAGGCCA TCAGCATGGG 721 TATTAGCAAG ATTAACGTGA ACACCGAGCT GCAACTGGCA TTCCGTGATG CTACGCGTAA 781 GTACATTGAA GCCGGCAAAG ATCTGGACGA CAAGACGAAA GGTTTCGATC CGCGCAAACT 841 GTTGAATCCG GGCTACCAGG CTCTGAAGGA AACGTTTAAT GAACTGACCG AGTGGTTCGG 901 CTGTAAAGGT AAAGCCTGAA GAGCTTAGAG ACCCCGCGTG GTTCTTACAG TATCGCTGAG 961 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 1021 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 1081 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 1141 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 1201 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1261 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1321 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1381 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1441 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1501 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1561 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 1621 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 1681 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 1741 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 1801 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 1861 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 1921 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 1981 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 2041 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 2101 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 2161 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 2221 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 2281 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2341 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2401 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 2461 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 2521 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 2581 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 2641 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 2701 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 2761 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 2821 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 2881 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA 2941 GGTGGAGTGG GAG //