LOCUS       Exported     2917 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2917
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      955..1002
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2850..2866
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2867..2898
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..883
                     /note=""
                     /translation="MFKKIDTFNFKKQSSIFKNLTLLVYKSFIKEPRSIIFMLVVPIFFSVMFFFIFGKEGKTLFNYALLPCLTVLTSLTPAIVEWKNSVFLKRIDITGVKKSMFIGSIWLVYLLAGIFFYIVVLSFNAILSIIVLGAGDSNSFLKAFSSIKIGYLLLSIILICATSIALATLFGGIANSSGSIQGIVMMVYFFSIFLSGIMLPTESLFKSQGMVIFTYFIPHKYPVFLYMYSFSKTGWTETYQGTQVTFQGAVNSFTSSWQPIVGSLAILGGLFTLTTFTFKWTAKR"
                     /label="Mfl645"
     primer_bind     complement(1007..1023)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1090..1678)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1869..2729)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2730..2834)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GTTTAAGAAA ATCGATACGT TCAATTTCAA
       61 GAAACAAAGC AGCATCTTTA AAAATCTGAC CTTGCTGGTC TATAAAAGCT TTATCAAGGA
      121 ACCGCGTAGC ATCATCTTCA TGTTGGTGGT TCCGATCTTT TTTAGCGTCA TGTTCTTTTT
      181 CATCTTCGGC AAAGAGGGCA AGACCCTGTT TAATTACGCC CTGCTGCCTT GCCTCACCGT
      241 ATTAACCAGC CTGACTCCGG CGATCGTCGA GTGGAAGAAT TCCGTCTTTC TGAAGCGTAT
      301 CGATATTACG GGCGTGAAAA AGAGCATGTT TATTGGTAGC ATCTGGCTGG TTTATCTGCT
      361 GGCTGGTATC TTCTTCTATA TTGTGGTGCT GAGCTTCAAC GCGATCCTGA GCATCATTGT
      421 TCTGGGCGCG GGTGACAGCA ACAGCTTCCT GAAGGCCTTT AGCAGCATTA AAATCGGTTA
      481 TCTGCTGCTG TCGATCATCC TGATTTGTGC AACTAGCATT GCGCTGGCAA CTCTGTTTGG
      541 TGGTATCGCG AATAGCTCTG GCAGCATCCA GGGTATTGTC ATGATGGTGT ATTTCTTTAG
      601 CATCTTCCTG AGCGGCATTA TGCTGCCTAC CGAGAGCCTG TTTAAAAGCC AGGGCATGGT
      661 CATCTTCACC TACTTTATCC CGCACAAATA TCCGGTGTTT CTGTATATGT ATAGCTTCAG
      721 CAAAACCGGT TGGACGGAAA CCTACCAGGG CACCCAAGTC ACCTTTCAAG GCGCTGTGAA
      781 TAGCTTTACC AGCAGCTGGC AGCCGATCGT CGGTTCTTTG GCCATCCTGG GCGGTTTATT
      841 TACCCTGACC ACCTTTACTT TTAAATGGAC TGCAAAACGC TGAAGAGCTT AGAGACCGTG
      901 CTATATCTTC CTCCCGGCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
      961 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1021 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1081 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1141 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1201 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1261 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1321 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1381 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1441 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1501 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1561 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1621 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1681 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1741 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1801 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1861 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     1921 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     1981 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2041 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2101 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2161 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2221 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2281 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2341 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2401 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2461 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2521 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2581 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2641 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2701 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2761 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2821 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     2881 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//