LOCUS       Exported     3662 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3662
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..3628
                     /label="pyrG"
                     /note="Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates."
                     /translation="MAKFVFVTGGVVSGLGKGITASSLGNLLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTDDGGETDLDLGHYERFIDEKLTKMSSTSAGKIYLETINAERRGDWGGQTIQVVPHITDSIKNKVYQAAKTSGADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVVFIHVALLLYLNASKEYKTKPIQMSVKELLSLGIQPDIIVQRTDKHSSKEIKEKISLFCNIPTSNVIDAIDKESIYEVPLEMYEQNLHGLVLDQLQIRTKKTDMESWIKFCEKIKASKEEFEVTFVGKYIELQDAYLSVIESLKIAGYEFKRKLKINWIQADKLNESNYKEVLQNAKGILVPGGFGDRGIEGMILASKFARENSIPYLGICLGMQIATISIARDWLNLPEANSTEFDHSGAVPIFDFIRGIDVQNLGGTLRLGAFYKTTIKEGTKAEKLYGSREVFERHRHRYEFNNEYKKALEEKGLVFSGIYEEKKLVEIIELPNHPFFVGSQYHPEFTSRPNKPNPLFKGFVEAIVNNK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATGTCCTT AGGCAGTCGT CGGTCTCAAA TGGCAAAGTT
     2041 TGTGTTCGTT ACCGGCGGTG TCGTTAGCGG TCTGGGCAAG GGCATTACGG CTTCCAGCCT
     2101 GGGTAATCTG CTGAAAGCTA GCGGTCTGAA AGTTTTTATG CAGAAGTTCG ATCCGTACTT
     2161 GAACGTAGAT CCAGGTACCA TGAGCCCGTA CCAGCATGGT GAGGTGTTTG TCACCGATGA
     2221 CGGCGGCGAA ACGGACCTGG ATCTGGGTCA TTATGAACGT TTCATTGATG AGAAGCTGAC
     2281 GAAGATGTCC TCCACCAGCG CGGGTAAGAT TTACCTGGAA ACCATCAACG CCGAGCGCCG
     2341 TGGTGACTGG GGTGGTCAAA CCATCCAAGT CGTTCCGCAC ATTACCGACA GCATTAAAAA
     2401 TAAGGTTTAC CAGGCGGCCA AGACCAGCGG CGCGGACGTT ATTATCAGCG AAATTGGCGG
     2461 TACCGTCGGT GACATTGAAA GCCAACCATT CATTGAGGCG ATTCGTCAAA TTCGTATGGA
     2521 GCAAGGTAAA GAAAACGTGG TGTTTATCCA TGTTGCATTG CTGCTGTACC TGAACGCGTC
     2581 TAAGGAGTAT AAGACTAAGC CGATCCAAAT GAGCGTTAAA GAGCTTCTTA GCCTGGGTAT
     2641 TCAGCCGGAC ATCATTGTAC AGCGCACCGA CAAGCATTCG AGCAAAGAGA TCAAAGAAAA
     2701 GATCAGCCTG TTTTGTAATA TTCCGACGAG CAACGTTATT GATGCAATCG ACAAAGAGAG
     2761 CATCTACGAG GTTCCGCTGG AGATGTATGA GCAAAATCTG CACGGTCTGG TCCTGGATCA
     2821 GCTGCAAATT CGTACCAAGA AAACGGACAT GGAAAGCTGG ATCAAATTCT GTGAGAAGAT
     2881 CAAGGCTAGC AAGGAGGAAT TTGAAGTCAC CTTCGTCGGC AAGTATATTG AACTGCAGGA
     2941 CGCGTACCTG TCCGTCATCG AAAGCCTGAA GATCGCCGGT TACGAATTTA AGCGTAAATT
     3001 GAAGATCAAC TGGATTCAAG CCGACAAGTT AAACGAGAGC AACTACAAGG AGGTCCTTCA
     3061 AAACGCCAAA GGTATCCTGG TTCCGGGTGG CTTTGGTGAC CGTGGCATCG AAGGCATGAT
     3121 TCTGGCGAGC AAATTCGCCC GTGAAAACAG CATTCCGTAC CTTGGTATCT GCCTGGGCAT
     3181 GCAAATTGCG ACCATTTCGA TCGCGCGTGA TTGGCTGAAT CTGCCGGAAG CGAATAGCAC
     3241 GGAATTCGAC CACAGCGGCG CAGTGCCGAT TTTTGACTTC ATCCGTGGTA TTGATGTGCA
     3301 AAACCTGGGC GGTACGCTGC GCCTGGGCGC TTTTTACAAA ACGACCATCA AGGAAGGCAC
     3361 CAAGGCTGAG AAGCTGTATG GCAGCCGCGA AGTGTTCGAG CGTCATCGTC ACCGTTACGA
     3421 ATTTAACAAC GAGTACAAGA AAGCGCTCGA GGAAAAAGGT CTGGTGTTCT CCGGTATTTA
     3481 CGAGGAGAAA AAGCTGGTTG AGATTATCGA GCTGCCGAAT CACCCGTTTT TTGTTGGTAG
     3541 CCAGTATCAC CCGGAATTTA CTAGCCGCCC GAACAAACCG AACCCGCTGT TTAAGGGTTT
     3601 TGTGGAAGCG ATCGTGAATA ACAAGTGAAG AGCTTAGAGA CCGGCTCGAT ACAGTTTCAC
     3661 GT
//