LOCUS       Exported     3820 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3820
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1858..1905
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3753..3769
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3770..3801
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1786
                     /label="Mfl652"
                     /note=""
                     /translation="MLLSSFKETFLQNFLSISTWQSLLAIVLFFGLMGGFWYGLRQIKIKFVYRIFIGISIGLIFGIAIQAIIGFPGGSWFSHDGQSAWALVDGNWLNISEYYIEKPNLEGMAEIKGNYVQIKLIIGLDGYEAIINGLTKLSLFKDAKGANVAFNSAISFSPEVKSGINWIIEFNTWASMLRQIFINGILLITIPVVFIAIFRVVAKPGSKGLGRISGKAVAILLINVAIAFTITFWIGYVLKIGKGLNFDQQIPGGSGRTDSKALPEIIWGYIPSNFVATLSATAIIPVIVLAALVGYATTFVRKKHPESMDNLMNFMDRAWDIVMSILMTFMKIMPLAVMAMLTTSITTRAIGALASIGLILGVGYLGLIIMLGVMTLILFLSGINVKAWWKHAWRPFVQAFSTQSSNATLPVTMDTLSNGMKIKDKVTSIVAPLSTSLGLVACAGVQAGLITSVLYTGSETVASMNIFAFFILGLFTTIVASIGIAGVPGTATVVTSAVLNGIGFGAFFAPVYAIFGAIDGLFDMGRTATNVTGGIFAATLVAKDELLFEEGTELISEKKLTALIENKEKKENLKKASKDKKKTNK"
     primer_bind     complement(1910..1926)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1993..2581)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2772..3632)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3633..3737)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GCTGCTGAGC AGCTTCAAAG AAACGTTCTT
       61 GCAAAACTTC CTGTCCATCA GCACCTGGCA AAGCCTGCTG GCGATTGTGC TGTTTTTCGG
      121 TTTAATGGGT GGCTTTTGGT ATGGTCTGCG CCAGATTAAG ATCAAATTTG TGTATCGCAT
      181 TTTCATCGGC ATTAGCATCG GTCTGATTTT TGGTATTGCT ATCCAGGCGA TCATCGGTTT
      241 TCCAGGCGGT AGCTGGTTTA GCCACGACGG TCAAAGCGCG TGGGCGCTGG TCGACGGCAA
      301 TTGGCTGAAT ATCAGCGAGT ACTACATTGA GAAACCGAAT CTGGAGGGTA TGGCGGAGAT
      361 TAAGGGTAAT TACGTGCAAA TTAAATTGAT TATTGGCCTG GACGGCTACG AGGCAATCAT
      421 CAACGGTCTG ACCAAACTGT CTCTGTTCAA AGATGCGAAG GGTGCGAACG TGGCCTTTAA
      481 TTCGGCGATC TCCTTTAGCC CGGAGGTTAA GAGCGGTATC AATTGGATCA TTGAATTCAA
      541 CACGTGGGCA AGCATGCTGC GTCAGATTTT TATTAACGGC ATCCTGCTGA TCACGATCCC
      601 GGTGGTTTTC ATTGCCATTT TTCGCGTGGT TGCAAAACCG GGTAGCAAGG GTCTGGGCCG
      661 CATCAGCGGT AAGGCCGTGG CTATTCTGCT GATCAATGTT GCGATCGCGT TCACGATCAC
      721 GTTCTGGATC GGTTATGTCC TGAAAATTGG TAAGGGTCTG AACTTCGATC AACAAATCCC
      781 GGGCGGCAGC GGTCGTACCG ACAGCAAGGC TTTGCCGGAG ATTATTTGGG GTTACATCCC
      841 AAGCAACTTT GTCGCCACGC TGTCTGCGAC CGCCATCATC CCTGTTATCG TGCTGGCAGC
      901 CCTGGTCGGC TATGCAACGA CCTTCGTCCG TAAGAAACAT CCGGAAAGCA TGGACAACCT
      961 GATGAATTTC ATGGACCGTG CGTGGGACAT TGTGATGTCT ATCTTGATGA CGTTCATGAA
     1021 GATCATGCCG CTGGCGGTGA TGGCCATGCT GACCACGAGC ATTACGACGC GCGCGATCGG
     1081 TGCCCTGGCC TCCATTGGCC TGATTCTGGG TGTCGGCTAT CTGGGCCTGA TTATTATGCT
     1141 GGGTGTGATG ACGTTGATCC TGTTCCTGTC GGGTATCAAC GTTAAGGCAT GGTGGAAACA
     1201 TGCCTGGCGT CCATTCGTTC AGGCATTCTC GACGCAGAGC AGCAACGCGA CCCTGCCGGT
     1261 TACCATGGAT ACCCTGAGCA ACGGCATGAA AATTAAAGAT AAGGTAACCA GCATTGTTGC
     1321 TCCACTGAGC ACGAGCTTAG GCCTGGTGGC GTGTGCGGGT GTCCAGGCGG GTCTGATTAC
     1381 CTCCGTGTTG TATACGGGCA GCGAAACCGT TGCAAGCATG AATATCTTCG CATTTTTTAT
     1441 CCTGGGTCTG TTCACCACCA TTGTGGCGAG CATCGGTATC GCGGGTGTTC CGGGCACGGC
     1501 AACGGTGGTC ACGAGCGCCG TGCTGAATGG CATCGGTTTT GGTGCGTTCT TTGCCCCGGT
     1561 TTATGCGATT TTCGGTGCGA TTGATGGTCT GTTCGACATG GGTCGCACGG CAACCAATGT
     1621 CACCGGTGGT ATTTTCGCCG CGACCCTGGT TGCGAAAGAC GAACTGCTGT TCGAGGAGGG
     1681 TACGGAACTG ATCAGCGAGA AAAAATTGAC CGCGCTGATT GAGAATAAAG AAAAGAAGGA
     1741 AAACCTGAAA AAAGCCTCAA AGGACAAGAA GAAAACCAAC AAGTGAAGAG CTTAGAGACC
     1801 GGACCTTGAC GCCTAATACT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1861 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1921 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1981 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     2041 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2101 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2161 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2221 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2281 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2341 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2401 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2461 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2521 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2581 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2641 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2701 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2761 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2821 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2881 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2941 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     3001 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     3061 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     3121 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     3181 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     3241 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     3301 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     3361 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3421 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3481 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3541 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3601 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3661 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3721 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3781 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//