LOCUS       Exported     3391 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3391
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1429..1476
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3324..3340
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3341..3372
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1357
                     /label="Mfl653"
                     /note=""
                     /translation="MKTVIIGGSATGMGVAAKLKRLNEQNEIVVIQDKDYVSLGACGIPYYIGHNFENPETLIARKIEKFEESQISVISNSKVNKIDFENKKVIFEESQISYDNLVIAVGAKPITPEIKNIDAKNIFTVNSKEDAIEIRKTINDDSNVLIIGSGLIGLEMVENIKHSTAAKISIIEKADRVLKNLFDEEFSELVEKEILNQEIELFKNNEVIEFVKDNNGKVTGAKTINGEIIKCDVVILSIGVIPNTSFLRDTDLELENNGAIKINEYCETNIPNVYAGGDCCSSLSYLYKNTRTFYLATVANKQAKIIANNVNKNKSSKFVGALGTTIIRFFDLELARTGENRMFIDMNSIETKEVLIKDKDHTNYVTGQEDIWLKVIIDVKTKEIINVQMIGKNKSVLRIYGLVGLIWSRSKVDETLEQIDLPYSPPFSRTTDIIHIALSKLI"
     primer_bind     complement(1481..1497)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1564..2152)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2343..3203)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3204..3308)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GAAAACCGTG ATTATTGGTG GTTCCGCGAC
       61 GGGCATGGGT GTTGCGGCGA AACTGAAGCG TCTGAACGAA CAGAACGAAA TCGTGGTCAT
      121 CCAGGATAAA GATTATGTAA GCTTGGGTGC GTGTGGTATC CCTTATTATA TCGGTCACAA
      181 CTTCGAAAAC CCGGAGACTC TTATTGCGCG TAAAATCGAA AAGTTCGAGG AAAGCCAGAT
      241 CTCGGTGATC AGCAATAGCA AGGTGAACAA AATTGACTTT GAGAACAAGA AGGTTATTTT
      301 TGAAGAGAGC CAAATCTCCT ATGACAACCT GGTTATTGCG GTCGGTGCGA AGCCGATTAC
      361 CCCAGAAATT AAGAATATTG ACGCCAAGAA CATTTTCACC GTGAACAGCA AGGAGGATGC
      421 CATTGAAATT CGCAAGACGA TTAATGACGA CTCCAACGTG CTGATCATCG GTTCCGGCCT
      481 GATCGGCCTG GAGATGGTGG AAAACATCAA GCACAGCACC GCGGCGAAAA TTTCTATTAT
      541 CGAAAAGGCA GACCGTGTTT TAAAGAACCT GTTTGATGAA GAGTTTAGCG AACTGGTGGA
      601 GAAGGAGATT CTGAATCAGG AGATTGAGTT GTTCAAGAAC AATGAGGTTA TTGAATTTGT
      661 GAAAGATAAC AATGGCAAAG TTACCGGTGC TAAGACGATT AATGGCGAGA TTATCAAATG
      721 CGACGTGGTT ATTCTGAGCA TTGGCGTTAT CCCGAATACT AGCTTCCTGC GCGACACGGA
      781 TCTGGAGCTG GAAAATAATG GCGCTATTAA AATCAATGAG TACTGTGAAA CGAATATCCC
      841 GAACGTTTAT GCGGGTGGTG ACTGCTGTTC CAGCCTGAGC TACTTGTACA AAAACACGCG
      901 TACCTTCTAC CTGGCAACCG TTGCGAATAA ACAAGCAAAG ATTATTGCAA ACAACGTGAA
      961 CAAAAATAAA AGCTCCAAGT TCGTTGGCGC GCTTGGTACG ACCATTATCC GCTTTTTCGA
     1021 TCTGGAGCTG GCCCGTACGG GCGAGAACCG CATGTTTATT GACATGAACA GCATTGAAAC
     1081 CAAGGAGGTA CTGATTAAAG ATAAGGATCA CACCAACTAC GTCACCGGCC AGGAGGATAT
     1141 CTGGCTGAAG GTCATTATCG ACGTGAAAAC GAAGGAAATC ATTAATGTTC AGATGATCGG
     1201 CAAGAACAAA AGCGTCCTGC GCATTTATGG TCTGGTTGGT CTGATTTGGA GCCGTTCGAA
     1261 GGTCGATGAA ACCCTGGAGC AGATTGATCT GCCATATAGC CCGCCTTTCA GCCGTACGAC
     1321 GGATATTATC CATATCGCGC TGTCCAAATT GATTTGAAGA GCTTAGAGAC CGTGTGGTCA
     1381 ATCCCATGTC TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1441 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1501 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1561 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1621 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1681 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1741 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1801 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1861 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1921 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1981 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2041 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2101 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2161 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2221 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2281 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2341 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2401 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2461 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2521 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2581 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2641 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2701 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2761 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2821 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2881 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2941 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     3001 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3061 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3121 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3181 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3301 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3361 AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA G
//