LOCUS Exported 2758 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2758 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 796..843 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2691..2707 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2708..2739 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..724 /label="Mfl656" /note="" /translation="MQKYIKNFKVSFLNDANSYLIVNEISKKAILIDGGYNANLILNYLINNNLDLTDIFITHFHHDHIVGLDYISIQTNANIHIHEYDFEYLFDVNVNGTQNGQKVDFTNQKISFKVFDKNLKLTINDFLINVIHKGGHSKGTTFYEVDNQYLFVGDTLFIDGFGYHKKLFSDAVNHPLKNMFDKTCNDEIFFESIKSIGNEFSEHLIYPGHWKEGFKIKEIIANNNHPFNKLK" primer_bind complement(848..864) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(931..1519) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1710..2570) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2571..2675) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GCAAAAGTAC ATTAAGAACT TCAAGGTGAG 61 CTTCCTGAAT GATGCGAACT CTTACTTAAT CGTTAATGAG ATTAGCAAGA AGGCAATCCT 121 GATTGATGGT GGTTATAATG CCAACCTGAT TTTGAACTAC CTGATCAATA ACAACCTGGA 181 CTTAACGGAT ATCTTTATTA CGCATTTCCA CCATGATCAC ATCGTTGGTT TGGATTACAT 241 CTCTATTCAA ACCAATGCGA ACATTCACAT TCATGAGTAT GACTTCGAAT ATCTGTTCGA 301 TGTTAATGTT AACGGCACGC AGAATGGTCA AAAGGTTGAT TTCACCAACC AGAAAATTTC 361 CTTCAAGGTT TTTGACAAAA ACCTGAAGCT GACTATTAAC GATTTCCTGA TCAACGTGAT 421 CCATAAAGGC GGTCATAGCA AAGGTACGAC GTTCTACGAA GTCGACAACC AGTATCTGTT 481 TGTTGGTGAC ACCTTGTTTA TTGATGGTTT TGGTTACCAC AAGAAACTGT TCTCGGATGC 541 TGTTAATCAC CCACTGAAGA ATATGTTCGA CAAGACGTGC AACGATGAAA TCTTTTTTGA 601 GAGCATTAAA TCGATTGGTA ACGAGTTCAG CGAGCATCTG ATCTACCCGG GTCACTGGAA 661 AGAAGGTTTC AAGATTAAAG AAATTATTGC GAACAACAAT CACCCGTTTA ACAAGCTGAA 721 GTGAAGAGCT TAGAGACCGC GCGGGTGATA TTACAACTCG CTGAGGTGTC AATCGTCGGA 781 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 841 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 901 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 961 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 1021 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 1081 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 1141 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1201 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1261 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1321 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1381 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 1441 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1501 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 1561 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 1621 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 1681 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 1741 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 1801 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 1861 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 1921 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 1981 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 2041 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 2101 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 2161 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2221 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2281 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2341 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 2401 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 2461 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 2521 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 2581 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 2641 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 2701 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG //