LOCUS       Exported     3797 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3797
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3763
                     /note=""
                     /translation="MCLFTWTCFASIFLIYFFITNLKKKEKKSMFKKRDKSSKVYEFWYLFLLIVGICIGSGIYVKNQELISQTKSPWIATVLWLTIGLVCVISIVVFMEIAKSTEKEGNGTVSNWCKLFINRKFASFVSVLYTTIYMPAYQSIFVSLTIAYFFAFTGITPDPKALLSVYILVGVSLFVLFAFVNVYSANISRKMQFFAMFIKFIPLIIAFFAGFLIAILGSNPKGNGMGMDSGTDLLFMNFLGGMGAILFSFDGYIFTANTQKSAKNKEVVPIAIISGLIFITMFYVLMAISLFLGGDGSVETVLIQVFSGFSEHPSDTSIKAASTVVYLIMFLICLLNINMFTHFGTSNLLSDHNMKLTYLRKGGNGFKKSAFTQMAISVTFYVALILVGGLSSHGKWAGMSTNVNINFFDPETQKVIYIAYLTSPMFYVGIMSSVCVVLIFIVISMLMIAAMWNRKTQKVKVSKVKFFWPSAIISTTLFLFFTICGLIVFLAPQLIDSSDMNWKTNGGMMFTIIFIVTLLATIVIWLLQERMFKINPFENGFEGEIDKDMKVNNWMAAKEAQELREINNETKTKIKST"
                     /label="Mfl664"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATCAGTTT CTTTGGCAGC CGGTCTCAAA TGTGCCTGTT
     2041 CACTTGGACC TGTTTCGCGA GCATTTTCCT CATCTACTTT TTTATTACCA ATCTGAAGAA
     2101 AAAGGAGAAA AAGAGCATGT TTAAAAAGCG CGACAAGAGC TCGAAAGTGT ATGAATTTTG
     2161 GTATCTGTTT CTGTTGATTG TTGGTATTTG CATTGGCTCC GGCATCTATG TTAAAAATCA
     2221 GGAGCTGATC AGCCAAACCA AATCCCCATG GATTGCAACC GTCCTGTGGC TGACCATTGG
     2281 CCTGGTCTGC GTGATCAGCA TCGTTGTGTT CATGGAGATT GCCAAAAGCA CCGAGAAAGA
     2341 AGGTAATGGT ACTGTTAGCA ATTGGTGCAA ACTGTTTATT AACCGTAAGT TCGCTTCTTT
     2401 TGTCAGCGTG CTGTACACCA CCATTTACAT GCCGGCGTAC CAGTCTATTT TCGTGAGCCT
     2461 GACGATTGCT TACTTTTTTG CATTCACGGG CATTACCCCG GACCCGAAAG CGTTGTTATC
     2521 TGTGTACATC CTGGTGGGTG TCAGCCTGTT CGTCCTGTTT GCATTCGTCA ACGTTTACTC
     2581 TGCCAACATC AGCCGTAAAA TGCAATTCTT CGCTATGTTC ATCAAATTCA TTCCGCTGAT
     2641 TATCGCGTTC TTCGCGGGCT TTCTGATTGC GATTTTAGGC AGCAACCCGA AAGGCAACGG
     2701 CATGGGCATG GACTCCGGTA CCGATCTGCT GTTTATGAAC TTTTTGGGCG GTATGGGCGC
     2761 AATCCTGTTT AGCTTCGACG GTTACATCTT TACGGCCAAC ACGCAGAAAA GCGCTAAGAA
     2821 TAAGGAAGTT GTGCCTATCG CCATCATCAG CGGCCTGATT TTCATCACGA TGTTTTACGT
     2881 CTTGATGGCC ATTAGCCTGT TCCTTGGTGG TGACGGCAGC GTCGAAACGG TTCTGATTCA
     2941 GGTTTTCAGC GGCTTCAGCG AGCATCCGAG CGACACGAGC ATCAAGGCTG CCTCCACCGT
     3001 TGTCTATCTC ATTATGTTCC TGATTTGCCT GCTGAATATC AATATGTTCA CCCACTTCGG
     3061 TACGTCTAAT CTGTTAAGCG ACCACAATAT GAAGCTGACG TATCTGCGTA AGGGTGGTAA
     3121 CGGTTTCAAG AAATCCGCGT TTACCCAAAT GGCCATTAGC GTGACGTTCT ATGTTGCGCT
     3181 GATCTTGGTG GGTGGTCTGA GCAGCCACGG CAAGTGGGCG GGCATGTCCA CGAATGTGAA
     3241 CATTAACTTT TTTGATCCAG AAACGCAGAA GGTCATTTAT ATTGCATATC TGACCAGCCC
     3301 GATGTTCTAC GTGGGTATCA TGTCCAGCGT GTGTGTGGTT CTGATTTTTA TTGTGATTAG
     3361 CATGCTGATG ATCGCAGCAA TGTGGAATCG TAAGACCCAA AAGGTTAAAG TTAGCAAGGT
     3421 TAAATTCTTC TGGCCAAGCG CGATTATCAG CACCACCTTG TTCCTGTTCT TTACCATCTG
     3481 CGGCCTGATC GTGTTCCTGG CCCCACAACT GATCGATTCT AGCGACATGA ACTGGAAAAC
     3541 GAATGGTGGC ATGATGTTTA CGATTATTTT CATTGTTACC CTGTTGGCGA CGATCGTTAT
     3601 TTGGCTGCTG CAGGAAAGAA TGTTCAAGAT CAACCCATTT GAAAACGGTT TTGAGGGTGA
     3661 GATCGATAAA GATATGAAAG TTAATAACTG GATGGCAGCG AAAGAAGCCC AGGAACTGCG
     3721 TGAGATTAAT AATGAAACCA AGACGAAGAT TAAGAGCACC TGAAGAGCTT AGAGACCCGC
     3781 TAAGATCCGT AGGTCTG
//