LOCUS       Exported     3436 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3436
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1474..1521
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3369..3385
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3386..3417
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1402
                     /translation="MKKLLLALLSTTIISTSATTVISCGTEKHFGEIYLITDSGKVDDKSFNQSTYEAGTEFVQEILGINQKIAYIQPESTAPKTMKRAYKTAESNNAKTLLLPGFHHAMPGEGEHQAAEVMKNSGSTIILDSNSAANNEIGVVFRGDVSGFYAGMSSIIYSLKNENYTNNTLSLGAYGGVSNPASVDNFIVGYLASIEVYNYLAKDTEFLNHFDIPEATRNVTVTKAQTGWPKSKDDTTWFSNSFLIGQSKLVLDNLRDTSKSIKPNVLMPVAGPQTSDALSYDTSWKVIGVDTNQAESYGKDAQNRFITSAEKDLKNAAIVSLAHTPEWMNNEQYPDILKKVDEGYKDKIQITKEVITDNKSEFVDVNIGDKELWTGTDVWVNGTMSYGGSNLLDEDLAIKIKKYFSAEALTEASKSLFSKYINGTIPSTGTIIAQEPIKDYADTILNELNKSTKKINE"
                     /label="Mfl669"
                     /note=""
     primer_bind     complement(1526..1542)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1609..2197)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2388..3248)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3249..3353)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAAAAGCTG CTGCTGGCAC TTCTGTCTAC
       61 TACGATCATC TCTACGAGCG CGACCACCGT CATCAGCTGC GGTACGGAAA AGCATTTTGG
      121 TGAGATTTAC CTGATCACCG ATAGCGGCAA AGTGGATGAT AAAAGCTTTA ATCAGAGCAC
      181 CTATGAGGCG GGCACCGAGT TTGTTCAGGA GATCCTGGGT ATCAACCAAA AAATCGCGTA
      241 TATTCAGCCG GAGAGCACGG CTCCAAAGAC CATGAAGCGC GCATACAAAA CCGCCGAGTC
      301 CAACAATGCC AAAACCCTGC TGCTGCCGGG CTTCCACCAC GCTATGCCGG GTGAAGGTGA
      361 ACACCAGGCG GCTGAGGTCA TGAAGAACTC CGGTAGCACC ATTATCCTGG ATTCTAATAG
      421 CGCAGCTAAT AATGAAATTG GCGTGGTTTT CCGTGGTGAT GTCTCCGGCT TCTATGCGGG
      481 TATGAGCAGC ATTATTTATA GCCTGAAGAA CGAGAATTAC ACCAACAACA CTTTGAGCTT
      541 AGGTGCGTAC GGCGGCGTGA GCAACCCGGC CAGCGTTGAC AACTTTATTG TTGGCTACCT
      601 CGCCAGCATC GAAGTGTACA ATTACTTGGC GAAAGATACC GAGTTTTTGA ATCACTTTGA
      661 CATTCCGGAG GCGACGCGTA ACGTTACGGT TACCAAAGCC CAGACCGGTT GGCCGAAAAG
      721 CAAGGATGAT ACCACCTGGT TCAGCAATAG CTTCCTGATT GGCCAGAGCA AGCTCGTGTT
      781 GGACAATTTG CGTGACACCA GCAAAAGCAT TAAGCCGAAT GTACTGATGC CGGTTGCGGG
      841 TCCTCAGACC TCGGATGCAC TGAGCTACGA TACCTCCTGG AAAGTGATTG GCGTGGATAC
      901 CAATCAGGCG GAGTCGTACG GCAAGGACGC CCAGAACCGC TTCATTACCA GCGCAGAGAA
      961 GGACCTGAAG AATGCGGCCA TCGTTAGCCT GGCGCACACC CCGGAATGGA TGAATAACGA
     1021 ACAGTATCCG GACATTCTGA AGAAAGTTGA TGAGGGTTAC AAAGACAAGA TTCAGATCAC
     1081 GAAGGAGGTG ATTACCGACA ATAAAAGCGA ATTCGTCGAT GTGAACATTG GTGATAAGGA
     1141 ATTGTGGACC GGTACCGACG TTTGGGTTAA TGGTACCATG AGCTACGGCG GTAGCAATCT
     1201 GCTCGACGAG GATCTGGCCA TCAAGATCAA GAAGTATTTC AGCGCCGAAG CACTGACCGA
     1261 AGCGTCTAAG AGCTTGTTCA GCAAATACAT TAACGGCACG ATTCCGAGCA CGGGCACCAT
     1321 CATTGCACAG GAACCGATCA AAGATTATGC GGACACGATC CTGAATGAGC TGAACAAGAG
     1381 CACCAAAAAG ATCAATGAGT GAAGAGCTTA GAGACCGGTA TATTTCTCCC CTCGGGCGCT
     1441 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1501 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1561 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1621 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1681 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1741 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1801 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1861 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1921 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1981 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2041 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2101 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2161 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2221 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2281 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2341 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2401 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2461 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2521 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2581 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2641 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2701 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2761 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2821 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2881 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2941 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3001 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3061 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3121 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3181 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3241 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3301 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3361 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3421 TCAGGTGGAG TGGGAG
//