LOCUS Exported 3944 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3944 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3910 /translation="MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVREIDALGGEMAKAADKTALQTKLLNSSRGPGVWALRVQSDKEEYSKYMQKIVEEQINLQLIESIATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSEILTGHEKFSSGPNEEPTSNGISESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQELCYLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESISGDAWYIQGFSTSMPTDVQLLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNINNLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPLILRRDEAYIGVMIDDLINKGVWEPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELEEIRFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVIETRFAGYVKTERKTVERLVKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRITGVNPADIQMLLFYLKNNYATEQK" /label="Mfl671" /note="NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34." primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGTATGT ATAGCGACCG CGGTCTCAAA TGAACAAGCA 2041 TTTCGATGTT ATCGTTATCG GTGGTGGTCA TGCGGGTGTG GAAGCCGCGC TGGCATCCGC 2101 GCGTCTGAAT AAAAAGACCG CACTGATCAA TCTGTATAAG GACCGTATCG CTGCCATGCC 2161 GTGTAACCCG AGCGTCGGTG GTCCGGCAAA AGGTATCGTG GTGCGTGAGA TCGACGCGCT 2221 GGGTGGTGAG ATGGCAAAGG CAGCCGATAA GACCGCCCTG CAAACCAAGT TGCTGAATAG 2281 CAGCCGTGGT CCGGGCGTTT GGGCTCTGCG CGTTCAAAGC GACAAGGAGG AATATAGCAA 2341 ATACATGCAG AAAATTGTCG AGGAACAGAT CAATCTGCAG CTGATTGAGT CAATCGCGAC 2401 CGACCTGATT ATTGAGAATA ATACTGTGAC CGGTGTTCTG TTGAAGGACG GTGAAATCTT 2461 GAAAAGCAAA TGCGTTGTTC TGACCACTGG TACGTATCTG AAATCCGAAA TTTTGACGGG 2521 CCACGAGAAG TTTAGCTCCG GCCCGAATGA GGAGCCTACC AGCAATGGCA TCAGCGAGTC 2581 CTTGAAGAAA TCTGGCGTCG AGCTGTTCCG TTTCAAGACG GGTACCCCGC CGCGCATCTA 2641 TAAGAATAGC GTGGACTTGT CCAAGGCTAT CAAGGAGCCG GGCACGGATG CAAAACTGGC 2701 GTTCAGCTTC TCGACCAATG AGTACACGCC GATTGAGAAA CAAGAGCTGT GCTACCTGAT 2761 TCACAGCAGC AGCGAAACCA AGAAGATCAT TGAGGACAAC TTAACCAAGA GCGCCATGTA 2821 CAGCGGTAAG GTTGAAAGCA TCGGTCCGCG TTACTGTCCG AGCTTCGAGG ACAAGATCGT 2881 CCGTTTTTCC AGCAAAGAAA CCCACCAAAT CTTTATTGAG CCGGAGAGCA TCTCTGGCGA 2941 CGCGTGGTAT ATTCAAGGTT TCAGCACCAG CATGCCGACG GACGTCCAAC TGCTGATGAT 3001 CCGTAGCCTG CCGGGTTTCG AAAAGGCAGA AGTTAAGACG TGGGCCTATG CTATCGAGTA 3061 CGATTGCGTT AACCCGATGC AACTGAACCC GAGCCTGGAG ATTAAAAATA TTAACAATCT 3121 GTTCACTGCG GGCCAAATTA ATGGTACCAG CGGTTACGAG GAGGCTGCAG GCCAAGGTCT 3181 GATTGCTGGC ATCAATGCGA GCCGTAAAAT TGATGGCCTG GATCCGCTGA TTTTACGCCG 3241 TGACGAGGCA TACATCGGCG TTATGATTGA TGATCTGATC AATAAGGGCG TGTGGGAGCC 3301 GTACCGCCTC CTGACCAGCC GTGCGGAGCA CCGTTTGCTG TTGCGTAATG ACAATGCGGA 3361 GCACCGCCTG AAGGAGAAAG GCTATGAGAT TGGCCTGATC AAACAGGCCG AATACAATGA 3421 ATACCTGAAG TATACGGAGG AGATCAATAA TGCTATTAAG GAGCTGGAGG AAATCCGTTT 3481 TACGCCTAAG AGCGAACTTG CCATCAAGCT GAAGGAGCTG GGTCAAGCGG ACCTGTCCCA 3541 TGGTTACAGC GGTATTGAGA TTCTTAAGAT CCCAACGGTG GACATTGAAG TCATGATTCC 3601 GTACATTAGC AGCCTGCAAA ACCTGAAAAA CAATCAGCTG CAAAGCATTG TGATCGAAAC 3661 CCGTTTCGCG GGTTATGTTA AGACTGAGCG TAAAACCGTT GAGCGTCTGG TTAAACTGGA 3721 GAAAAAGAAG ATTCCGTCGG ATATCGATTA CGACAAGGTT GAAAACTTGG CAACTGAAGC 3781 TCGTCAGAAA CTGAAAAAGG TCAAGCCGCT GAATATCGGT CAGGCCAGCC GCATCACCGG 3841 TGTCAATCCG GCTGATATCC AAATGTTGCT GTTCTATCTG AAAAATAACT ACGCAACCGA 3901 ACAGAAGTGA AGAGCTTAGA GACCCCTACG GTTAAGGCTT GTCT //