LOCUS       Exported     3944 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3944
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3910
                     /translation="MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVREIDALGGEMAKAADKTALQTKLLNSSRGPGVWALRVQSDKEEYSKYMQKIVEEQINLQLIESIATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSEILTGHEKFSSGPNEEPTSNGISESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQELCYLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESISGDAWYIQGFSTSMPTDVQLLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNINNLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPLILRRDEAYIGVMIDDLINKGVWEPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELEEIRFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVIETRFAGYVKTERKTVERLVKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRITGVNPADIQMLLFYLKNNYATEQK"
                     /label="Mfl671"
                     /note="NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGTATGT ATAGCGACCG CGGTCTCAAA TGAACAAGCA
     2041 TTTCGATGTT ATCGTTATCG GTGGTGGTCA TGCGGGTGTG GAAGCCGCGC TGGCATCCGC
     2101 GCGTCTGAAT AAAAAGACCG CACTGATCAA TCTGTATAAG GACCGTATCG CTGCCATGCC
     2161 GTGTAACCCG AGCGTCGGTG GTCCGGCAAA AGGTATCGTG GTGCGTGAGA TCGACGCGCT
     2221 GGGTGGTGAG ATGGCAAAGG CAGCCGATAA GACCGCCCTG CAAACCAAGT TGCTGAATAG
     2281 CAGCCGTGGT CCGGGCGTTT GGGCTCTGCG CGTTCAAAGC GACAAGGAGG AATATAGCAA
     2341 ATACATGCAG AAAATTGTCG AGGAACAGAT CAATCTGCAG CTGATTGAGT CAATCGCGAC
     2401 CGACCTGATT ATTGAGAATA ATACTGTGAC CGGTGTTCTG TTGAAGGACG GTGAAATCTT
     2461 GAAAAGCAAA TGCGTTGTTC TGACCACTGG TACGTATCTG AAATCCGAAA TTTTGACGGG
     2521 CCACGAGAAG TTTAGCTCCG GCCCGAATGA GGAGCCTACC AGCAATGGCA TCAGCGAGTC
     2581 CTTGAAGAAA TCTGGCGTCG AGCTGTTCCG TTTCAAGACG GGTACCCCGC CGCGCATCTA
     2641 TAAGAATAGC GTGGACTTGT CCAAGGCTAT CAAGGAGCCG GGCACGGATG CAAAACTGGC
     2701 GTTCAGCTTC TCGACCAATG AGTACACGCC GATTGAGAAA CAAGAGCTGT GCTACCTGAT
     2761 TCACAGCAGC AGCGAAACCA AGAAGATCAT TGAGGACAAC TTAACCAAGA GCGCCATGTA
     2821 CAGCGGTAAG GTTGAAAGCA TCGGTCCGCG TTACTGTCCG AGCTTCGAGG ACAAGATCGT
     2881 CCGTTTTTCC AGCAAAGAAA CCCACCAAAT CTTTATTGAG CCGGAGAGCA TCTCTGGCGA
     2941 CGCGTGGTAT ATTCAAGGTT TCAGCACCAG CATGCCGACG GACGTCCAAC TGCTGATGAT
     3001 CCGTAGCCTG CCGGGTTTCG AAAAGGCAGA AGTTAAGACG TGGGCCTATG CTATCGAGTA
     3061 CGATTGCGTT AACCCGATGC AACTGAACCC GAGCCTGGAG ATTAAAAATA TTAACAATCT
     3121 GTTCACTGCG GGCCAAATTA ATGGTACCAG CGGTTACGAG GAGGCTGCAG GCCAAGGTCT
     3181 GATTGCTGGC ATCAATGCGA GCCGTAAAAT TGATGGCCTG GATCCGCTGA TTTTACGCCG
     3241 TGACGAGGCA TACATCGGCG TTATGATTGA TGATCTGATC AATAAGGGCG TGTGGGAGCC
     3301 GTACCGCCTC CTGACCAGCC GTGCGGAGCA CCGTTTGCTG TTGCGTAATG ACAATGCGGA
     3361 GCACCGCCTG AAGGAGAAAG GCTATGAGAT TGGCCTGATC AAACAGGCCG AATACAATGA
     3421 ATACCTGAAG TATACGGAGG AGATCAATAA TGCTATTAAG GAGCTGGAGG AAATCCGTTT
     3481 TACGCCTAAG AGCGAACTTG CCATCAAGCT GAAGGAGCTG GGTCAAGCGG ACCTGTCCCA
     3541 TGGTTACAGC GGTATTGAGA TTCTTAAGAT CCCAACGGTG GACATTGAAG TCATGATTCC
     3601 GTACATTAGC AGCCTGCAAA ACCTGAAAAA CAATCAGCTG CAAAGCATTG TGATCGAAAC
     3661 CCGTTTCGCG GGTTATGTTA AGACTGAGCG TAAAACCGTT GAGCGTCTGG TTAAACTGGA
     3721 GAAAAAGAAG ATTCCGTCGG ATATCGATTA CGACAAGGTT GAAAACTTGG CAACTGAAGC
     3781 TCGTCAGAAA CTGAAAAAGG TCAAGCCGCT GAATATCGGT CAGGCCAGCC GCATCACCGG
     3841 TGTCAATCCG GCTGATATCC AAATGTTGCT GTTCTATCTG AAAAATAACT ACGCAACCGA
     3901 ACAGAAGTGA AGAGCTTAGA GACCCCTACG GTTAAGGCTT GTCT
//