LOCUS       Exported     3959 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3959
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3925
                     /translation="MEQNKALYRKYRPSNFGDIAGHQNVVEILKNELKNNKISHSFLFAGQRGTGKTSIARILAKSVNCLNIKDGLACEVCESCIASNEQRNPDIIEMDAASNNGVDEIREIKNNINSLPFIGKYKIYIIDEVHMLTKAAFNALLKTLEEPPVHAIFILATTEYAKIPATILSRCQIFNFKKIDRISLMNRLSFICENEGYKIDKAVLEEIAIISEGSLRDASNVIEQLMTVTSDHITVEDLKSVFYVATKNEKMKILTNILNNDPATIINYFEKANNQGMDFDVFTLSLIEIVKEIIEFKFTNDENVLNILDKSDLNEFAQVNVKSLFEVADNLSEAYAKTKGTNINFNYLLISLLKTLKEPTEHKIKIEESVLTIQENIIVKEVIAEPVHIQEPEKQILIEKVEEIKEEVIEQPIKTFEEKITQVKDIEIKEESVSIDDDYELKTLTNLKIQLNKNLVDNEEKKTYKVEINEVINLLVGANKNKREDIENKINSFFKVDEQDNLLNPDLAKKYINFFNFKVIAASQNEILLRADDFENVNNLLVFLQNEEYRESIQKEFGKALFLPIDETLWNEVKISFKRLKENNQLPSYEEINYDSYFDKKKQSMVLSHFDDKTIEAASRLFNIDELEIED"
                     /label="Mfl678"
                     /note="DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCCCAG AAGGATACAA CGGTCTCAAA TGGAGCAGAA
     2041 TAAGGCCCTG TATCGTAAGT ACCGCCCGTC AAACTTCGGC GATATCGCGG GTCATCAGAA
     2101 CGTGGTTGAG ATTCTGAAGA ATGAATTGAA GAATAATAAG ATCAGCCATT CCTTTCTCTT
     2161 TGCTGGTCAA CGTGGCACGG GTAAGACCAG CATTGCGCGT ATCCTGGCAA AAAGCGTGAA
     2221 CTGTTTGAAC ATCAAGGATG GTCTGGCTTG TGAGGTTTGT GAGAGCTGCA TTGCGTCTAA
     2281 TGAGCAGCGT AATCCGGATA TTATCGAGAT GGACGCCGCG TCCAACAACG GCGTTGACGA
     2341 GATCCGTGAA ATCAAGAACA ACATCAATAG CCTGCCGTTC ATTGGTAAAT ATAAGATTTA
     2401 CATCATCGAT GAAGTGCATA TGCTGACCAA AGCCGCGTTC AATGCGCTGC TGAAAACCCT
     2461 GGAAGAACCG CCGGTGCACG CAATCTTTAT TCTGGCCACC ACCGAGTATG CCAAAATTCC
     2521 GGCGACCATC CTGTCACGCT GCCAAATTTT CAATTTTAAA AAGATTGACC GTATTAGCCT
     2581 GATGAATCGC CTGAGCTTTA TTTGTGAAAA CGAAGGTTAC AAAATCGACA AAGCGGTTCT
     2641 GGAAGAAATC GCTATCATCA GCGAGGGCTC CCTGCGTGAC GCGTCCAACG TTATCGAACA
     2701 GCTGATGACC GTTACGTCGG ATCACATTAC CGTGGAAGAT CTGAAAAGCG TGTTCTACGT
     2761 TGCGACCAAA AATGAGAAAA TGAAGATTCT GACCAATATC TTGAATAACG ATCCTGCCAC
     2821 CATCATTAAT TATTTTGAAA AAGCAAATAA TCAGGGCATG GACTTCGACG TTTTCACCCT
     2881 GAGCTTGATC GAAATTGTCA AGGAAATTAT CGAGTTTAAG TTTACGAACG ACGAGAATGT
     2941 TTTGAATATC CTGGACAAAA GCGATCTGAA CGAGTTCGCC CAGGTGAATG TTAAGTCCTT
     3001 GTTCGAGGTG GCGGACAACT TAAGCGAGGC CTACGCTAAA ACTAAAGGCA CCAATATTAA
     3061 CTTTAATTAT CTGCTGATTT CATTGTTAAA GACCCTGAAG GAACCTACCG AGCACAAAAT
     3121 TAAGATCGAG GAGAGCGTGC TGACCATTCA GGAGAATATT ATTGTTAAAG AAGTGATCGC
     3181 AGAGCCGGTG CATATTCAGG AACCTGAGAA GCAGATTTTG ATTGAGAAAG TTGAAGAAAT
     3241 TAAAGAAGAA GTCATCGAAC AACCGATTAA GACCTTCGAG GAGAAAATCA CCCAGGTGAA
     3301 AGATATCGAG ATTAAAGAGG AGAGCGTTTC TATCGACGAT GACTATGAGC TGAAAACCCT
     3361 GACTAACCTG AAAATCCAGC TTAACAAGAA CCTGGTTGAT AATGAGGAAA AGAAAACCTA
     3421 TAAGGTCGAG ATCAACGAGG TCATCAATCT GCTGGTCGGC GCGAACAAAA ACAAGCGTGA
     3481 GGACATTGAA AATAAAATTA ATAGCTTCTT CAAAGTTGAC GAGCAGGACA ATCTGCTGAA
     3541 TCCAGACCTG GCAAAGAAAT ACATTAACTT TTTCAATTTC AAAGTGATCG CGGCGAGCCA
     3601 AAACGAAATC CTGCTGCGTG CGGATGACTT TGAAAATGTC AACAACCTGT TAGTGTTTCT
     3661 GCAAAACGAG GAGTATCGCG AGAGCATCCA AAAGGAATTC GGTAAGGCAC TGTTTCTGCC
     3721 GATCGATGAA ACGCTGTGGA ACGAAGTTAA GATTTCTTTC AAACGTTTGA AGGAGAATAA
     3781 CCAGCTGCCG TCGTACGAAG AGATTAACTA TGACAGCTAT TTTGATAAGA AGAAACAAAG
     3841 CATGGTGCTG TCTCACTTCG ACGACAAAAC CATCGAAGCT GCCTCTCGCC TGTTCAATAT
     3901 TGATGAACTG GAAATCGAAG ATTGAAGAGC TTAGAGACCG CTGGGCACTA TGCTTTACT
//