LOCUS       Exported     3319 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3319
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1357..1404
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3252..3268
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3269..3300
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1285
                     /label="Mfl681"
                     /note=""
                     /translation="MLILYNENQLFMSNPKKNGVMSYMNSNSTTKSKKTFWQKMSDSNDRKRILKLIWKWFKILGFLFIIISMIWGCVQMYQDPYLIQEVTDMTGRRVYMPGVSFEIVISALGERPSQNWAVIVTGLNGQQYAYNVISSWSEAFSKTGSPFYGFFVYPTALLLNSLIKGMSGTLNPELGNTSYGVSVIFAILFTVIIIKSISLAFTWKSQVNQHKQQAFQLKQADIQAKYKGDKSPQARQKMSAEMQALQKKEGFSPFSSMAGAFASMPFLFAIYAIVRSTKSLKIANVGMIALIEKPWSQMLNGEVIYLSLLAVYLPLQIISMLLPLVLQSIKQKSITLTEAQRKSRKKQFIMQGVFAFVFIFVVASVASGVAIYWIFSSTFQIIQTLGFFFWNQHKANRSVQEVQRRKRQEERRLKKLSK"
     primer_bind     complement(1409..1425)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1492..2080)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2271..3131)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3132..3236)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGTCCTTA GGCAGTCGTC GGTCTCAAAT GCTGATTCTG TATAATGAGA ACCAACTGTT
       61 TATGAGCAAT CCTAAGAAGA ATGGTGTCAT GAGCTACATG AATAGCAATA GCACTACCAA
      121 GAGCAAGAAA ACCTTCTGGC AAAAGATGTC TGACAGCAAT GATCGTAAAC GTATCCTGAA
      181 GTTGATTTGG AAATGGTTTA AAATTCTGGG TTTTTTGTTC ATCATTATTA GCATGATCTG
      241 GGGCTGCGTT CAAATGTACC AGGATCCGTA TCTGATTCAG GAGGTGACCG ACATGACCGG
      301 CCGTCGTGTC TATATGCCGG GTGTCAGCTT TGAGATTGTG ATTAGCGCCC TGGGTGAACG
      361 CCCGAGCCAG AACTGGGCGG TTATCGTCAC CGGCCTGAAT GGTCAACAAT ATGCGTACAA
      421 CGTCATTAGC AGCTGGAGCG AAGCTTTTAG CAAAACGGGC AGCCCATTCT ACGGCTTCTT
      481 TGTGTACCCT ACTGCGCTGC TGTTGAACAG CCTGATTAAA GGCATGTCTG GCACCTTGAA
      541 TCCAGAGCTG GGTAACACGA GCTATGGTGT TAGCGTTATT TTCGCCATTC TGTTTACCGT
      601 GATTATCATT AAAAGCATTA GCCTGGCGTT CACCTGGAAA AGCCAAGTCA ATCAGCACAA
      661 GCAACAGGCC TTTCAACTGA AACAAGCAGA TATTCAGGCC AAGTATAAGG GCGATAAAAG
      721 CCCGCAAGCC CGTCAAAAGA TGAGCGCGGA GATGCAGGCG CTGCAAAAGA AAGAAGGTTT
      781 CTCCCCGTTT AGCAGCATGG CTGGTGCATT TGCCTCCATG CCGTTCCTGT TTGCGATCTA
      841 CGCAATTGTG CGTTCCACGA AATCCCTGAA GATCGCGAAT GTTGGTATGA TCGCGCTGAT
      901 TGAAAAGCCA TGGAGCCAGA TGCTGAATGG TGAGGTGATC TACCTGTCAT TACTGGCAGT
      961 GTATCTGCCG CTGCAGATTA TTAGCATGCT GCTGCCGTTG GTGTTGCAGT CCATCAAGCA
     1021 AAAGAGCATT ACGCTGACCG AAGCGCAGCG CAAGAGCCGT AAGAAGCAAT TCATTATGCA
     1081 GGGTGTCTTT GCATTTGTTT TTATCTTTGT GGTCGCTTCT GTCGCGAGCG GTGTTGCAAT
     1141 TTACTGGATT TTTTCAAGCA CGTTTCAAAT CATTCAAACC CTGGGCTTCT TTTTCTGGAA
     1201 TCAGCACAAG GCAAACCGTT CTGTTCAAGA AGTTCAACGT CGTAAACGCC AAGAGGAGCG
     1261 CCGCCTGAAA AAACTTAGCA AGTGAAGAGC TTAGAGACCC TGTATCATGG TTCCCTCGGC
     1321 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1381 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1441 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1501 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1561 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1621 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1681 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1741 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1801 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1861 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1921 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1981 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2041 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     2101 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     2161 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2221 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2281 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2341 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2401 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2461 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2521 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2581 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2641 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2701 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2761 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2821 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2881 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2941 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     3001 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     3061 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     3121 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3181 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3241 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3301 TGGTCAGGTG GAGTGGGAG
//