LOCUS       Exported     2989 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2989
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1027..1074
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2922..2938
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2939..2970
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..955
                     /label="MG_RS00100"
                     /note="Specifically catalyzes the removal of N-terminal proline residues from peptides."
                     /translation="MNTKLNVKGYLNVGDNHQLYYWTQGNPNGKPVLYIHGGPGSGTDEGCLKYFDLETTWIILLDQRGCGKSKTNDIFYENNTDKLVSDFEILRQKLNIKNWTLFGGSWGSALALVYAIKHPQVVDKIFLRALFLAREKDWSEALMGLGKMFYPYEHQRFMDSIPKAYQNSYEQIVNYCYDQFQNGDESTKEKLAKAWVDWESTLLSPINKIHSTATDFKLVEKLALLECHYAVNKSFLDENFILDNISVLKNKSIYLAHGRFDLICPLYQPLALKQAFPELQLYVTNNAGHSGSDANNLATIKHLLKTYL"
     primer_bind     complement(1079..1095)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1162..1750)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1941..2801)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2802..2906)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GAACACCAAG CTGAACGTGA AGGGCTATCT
       61 GAACGTTGGT GACAACCATC AGCTGTACTA TTGGACTCAG GGTAACCCTA ACGGCAAGCC
      121 GGTTCTGTAC ATTCACGGTG GTCCAGGTAG CGGTACCGAC GAAGGTTGCC TGAAGTACTT
      181 CGATTTAGAA ACCACGTGGA TCATCTTGCT GGACCAACGT GGTTGTGGTA AGTCGAAAAC
      241 GAACGATATC TTCTACGAAA ATAACACGGA TAAGTTGGTC AGCGATTTCG AAATTCTCCG
      301 CCAAAAGCTG AACATTAAGA ATTGGACCCT GTTTGGTGGC AGCTGGGGTT CTGCACTGGC
      361 TCTGGTCTAC GCGATCAAAC ACCCTCAAGT TGTCGACAAG ATCTTCCTGC GTGCCTTGTT
      421 CCTGGCCCGC GAAAAAGACT GGTCTGAAGC CCTGATGGGC CTGGGTAAAA TGTTTTATCC
      481 GTACGAGCAT CAGCGTTTTA TGGACAGCAT CCCGAAGGCG TACCAAAATT CCTACGAGCA
      541 GATTGTCAAT TATTGTTATG ATCAATTTCA GAATGGTGAC GAGAGCACGA AAGAGAAACT
      601 GGCGAAGGCG TGGGTCGATT GGGAGTCGAC GCTGCTGAGC CCAATCAATA AGATTCACAG
      661 CACGGCAACG GACTTTAAGC TGGTCGAAAA GCTGGCCCTG CTGGAATGCC ACTACGCGGT
      721 TAATAAGAGC TTCCTGGATG AAAATTTTAT TCTGGATAAT ATCTCGGTGC TGAAGAACAA
      781 AAGCATTTAC CTGGCGCACG GCCGTTTCGA CCTCATTTGT CCGCTGTATC AACCGCTGGC
      841 GCTGAAACAA GCATTCCCGG AACTGCAGCT GTACGTTACC AACAACGCGG GTCATAGCGG
      901 TTCGGATGCG AATAATCTGG CAACCATCAA GCACTTGCTC AAGACCTATC TGTGAAGAGC
      961 TTAGAGACCG GGCACTCAAT ATACCCTGTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1021 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1081 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1141 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1201 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1261 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1321 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1381 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1441 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1501 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1561 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1621 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1681 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1741 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1801 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1861 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1921 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1981 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2041 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2101 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2161 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2221 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2281 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2341 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2401 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2461 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2521 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2581 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2641 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2701 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2761 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2821 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2881 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2941 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//