LOCUS       Exported     3733 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3733
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1771..1818
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3666..3682
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3683..3714
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1699
                     /label="MG_RS00180"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /translation="MNLENEMCFNQRILIGSISTEQLNKTIVIIGWIKRIKKLGEINFIIVGDKSGTIQVTCKDKEQIQQLTREDIVIVKAKLQRLDSVRFELINPTIKLFSKSKTPPLIIEDETDALEEVRLKYRYLDLRRRLMQKRLLLRHQFILAIRNWFNQQGFIEIETPTLSKSTPEGAQDFLVPARIRKDCFYALVQSPQIYKQLLMIAGVEKYFQIARVYRDEDSRKDRQPEHTQIDFEISFCNQKMIMNLVEKLFFSVFLDVFQIKIKKTFPVFKFSELFERFGSDKPDLRYGFEIKDFTSLFQDHQNQFTKLIEAKGIIGGIELTNIELSTDKIKALRKIAKDHDVSLEVHNKNNSTLKTSIKCDEKNTLLLVANKSKKKAWTALGAIRNELKYHLDIVKPNQYSFCWVVDFPLYDFDEKTNQWISNHNIFSKPKQEWIDNFESNKNEALSEQFDLVLNGFEIGSGSIRINDPIVQKRLMNSLNIDPNKFAFLLEAYQYGAPVHGGMGLGIDRLMMILNQTDNIREVIAFPKNNHGIEVHTNAPDKIDKEEVKWWIKELVK"
     primer_bind     complement(1823..1839)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1906..2494)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2685..3545)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3546..3650)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GAACCTGGAG AATGAAATGT GCTTTAACCA
       61 AAGAATCCTG ATCGGTTCGA TCAGCACCGA ACAGCTGAAC AAGACCATCG TTATCATCGG
      121 TTGGATCAAG CGTATTAAGA AACTGGGTGA GATCAACTTT ATTATCGTGG GCGACAAGAG
      181 CGGTACTATT CAGGTTACGT GCAAAGACAA AGAGCAGATC CAACAGCTCA CCCGTGAGGA
      241 TATCGTTATC GTGAAGGCGA AGCTGCAGCG TCTGGACAGC GTTCGCTTTG AGCTGATTAA
      301 CCCGACCATT AAATTGTTCT CCAAGAGCAA GACCCCGCCG CTGATCATTG AGGATGAAAC
      361 GGACGCACTG GAAGAGGTTC GTCTGAAATA CCGCTATCTG GATCTGCGTC GTCGTCTGAT
      421 GCAAAAGCGT CTGCTGCTGC GCCACCAGTT CATTCTCGCT ATCCGTAATT GGTTCAACCA
      481 GCAAGGTTTC ATCGAAATCG AAACGCCGAC CCTGAGCAAG AGCACGCCGG AGGGCGCGCA
      541 GGACTTTTTG GTGCCGGCGA GAATCAGAAA GGACTGCTTT TATGCGCTGG TCCAAAGCCC
      601 GCAAATTTAC AAACAGCTGT TGATGATTGC GGGTGTCGAA AAGTATTTCC AGATTGCGCG
      661 TGTGTACCGT GACGAGGACA GCCGTAAGGA CCGCCAGCCA GAACATACGC AGATCGATTT
      721 CGAGATTTCG TTTTGCAATC AAAAAATGAT CATGAATCTG GTCGAGAAAC TGTTTTTCAG
      781 CGTTTTTCTG GACGTTTTCC AGATCAAAAT CAAGAAAACC TTTCCGGTTT TTAAGTTTTC
      841 AGAACTGTTC GAGCGCTTCG GCTCCGACAA GCCGGATCTG CGTTACGGTT TCGAAATCAA
      901 AGACTTTACT AGCCTGTTTC AAGATCACCA GAATCAGTTT ACGAAACTGA TCGAGGCGAA
      961 AGGTATTATT GGTGGTATCG AGCTGACCAA TATCGAACTG AGCACGGATA AAATTAAGGC
     1021 GCTGCGCAAG ATCGCGAAAG ACCACGATGT CAGCCTGGAG GTTCACAATA AGAATAACTC
     1081 CACCTTAAAG ACCAGCATTA AGTGTGATGA GAAAAACACC CTGTTGCTGG TTGCTAATAA
     1141 ATCTAAGAAA AAGGCCTGGA CGGCGTTGGG TGCCATTCGT AACGAACTGA AATACCACCT
     1201 GGATATTGTC AAACCGAATC AGTATTCCTT CTGTTGGGTC GTAGACTTCC CGCTGTACGA
     1261 TTTTGACGAG AAAACCAATC AATGGATTAG CAACCACAAT ATCTTTAGCA AACCTAAACA
     1321 GGAGTGGATT GATAACTTCG AAAGCAATAA AAATGAGGCG CTGAGCGAAC AGTTCGACTT
     1381 GGTGCTGAAT GGTTTCGAGA TCGGTAGCGG CTCGATCCGT ATCAATGATC CGATCGTGCA
     1441 GAAGCGTCTG ATGAACTCTC TGAACATTGA CCCGAACAAA TTCGCGTTTC TGCTGGAAGC
     1501 GTACCAATAC GGCGCGCCGG TCCATGGTGG CATGGGTCTG GGCATCGACC GCCTGATGAT
     1561 GATTCTGAAC CAAACCGACA ACATTCGTGA AGTTATTGCC TTTCCGAAGA ACAACCATGG
     1621 TATCGAGGTG CACACGAATG CACCGGACAA GATCGACAAA GAAGAGGTTA AGTGGTGGAT
     1681 CAAGGAGCTG GTCAAGTGAA GAGCTTAGAG ACCCGGCTGG CTGTAAGAAT ACTCGCTGAG
     1741 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1801 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1861 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1921 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1981 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     2041 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     2101 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     2161 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     2221 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2281 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2341 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2401 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2461 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2521 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2581 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2641 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2701 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2761 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2821 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2881 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2941 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     3001 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     3061 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     3121 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     3181 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     3241 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     3301 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3361 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3421 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3481 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3541 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3601 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3661 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     3721 GGTGGAGTGG GAG
//