LOCUS       Exported     3412 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3412
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1450..1497
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3345..3361
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3362..3393
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1378
                     /label="MG_RS00185"
                     /note="Derived by automated computational analysis usinggene prediction method: Protein Homology."
                     /translation="MDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFVWNHDFAKEILLNVFNGFVNSVIEVKKNKLLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPNNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDMWNVLDNIIPRLKNLILMRGATLYLRADSGNYQTLICNPNYKKQDKSTWAMIDYLDHHFSSTINKKGYKVLNKKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKRNNRWIGVKKTPITDLSKSSKGGRYKTKRLITVY"
     primer_bind     complement(1502..1518)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1585..2173)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2364..3224)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3225..3329)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GGATAAACGC ACCTCTGAGA TCGAGTTCCA
       61 CTTGAAAAAC CTGTTAGCAT GCGACGCGTA CAAACTGAGC CATCGTCTGA TGTATCCGCA
      121 AGACACGCAG AATCTGTATT CCATGCTGAC CGCCCGTGGT GTCTACGGCG ACTTTAAGGA
      181 GTTCGTATGG AATCACGACT TCGCAAAGGA GATTTTGCTG AACGTGTTCA ATGGTTTCGT
      241 TAACAGCGTC ATCGAGGTGA AGAAAAATAA ACTGCTGGCA GCGGCGCTGA CGGACAAACT
      301 GGTTTCTGTT TTCAACGACC ACGAATTGGC GAACGAGTTC ACCCAACATA TCTGTCACCT
      361 GGCGAGCTTC CTGGAGAAAA ATAAGAAAAT GCCGCTGGTG GCTAAGATTC ATGAATCCGA
      421 CCAAAGCCTG CCGTTCCGCA CTCCGCTGAT CACTATCGAG GGTGTGGAGA ACATTCCGAA
      481 CAATTTCGTG TGGCTGGTTA ACTACTTTGA AACGGTTCTT CTGGAGAATA TCTGGCTGTT
      541 TCAAACCGCA AGCACGGTCG CCAAACGTAT TAAGAGCTTG CTGGAGAAAT ATGCAAAAGA
      601 AACCGCGGAC GAAACCAGCT TTATTAACTT TCAGTGCCAT GACTTTAGCA TGCGTGGTAT
      661 GAGCAGCCTG CAGAGCGCGC TGTACGTCGC CCGCGCGCAT TTGCAGTACT TCACCGGCAG
      721 CGATACCATC CTGGGTGGTG ATAACAGCCG CTCGATCCTG GCTTCCGAAC ATTCCGTTAT
      781 GTGTGCGGAC GGCAGCAAGC ACGAGCTCAA AACTTTTCAG CGCTTGCTGG AAAAATTCAA
      841 GGATAAGAAG CTGAGCCTGG TGATCGATTC GTATGACATG TGGAACGTGT TGGATAACAT
      901 CATTCCGCGC CTGAAGAATT TGATTCTGAT GCGTGGCGCG ACGTTGTATC TGCGTGCCGA
      961 CAGCGGCAAT TACCAAACTT TGATCTGTAA TCCGAACTAC AAGAAGCAGG ACAAAAGCAC
     1021 GTGGGCCATG ATCGATTATC TGGACCATCA CTTTTCTAGC ACCATTAACA AAAAGGGCTA
     1081 CAAGGTGCTG AATAAAAAGA TTGGTATTAT TTATGGTGAT GGTATTACGT ACCAAAAGAT
     1141 CGAGTGGATT CTGAACTGTC TGAAAAATCA CGGTTACTGC AGCTCCAACA TTATCTTCGG
     1201 CGTCGGCTCC TCCACCTACC AGAATTTGAA CCGCGACACG CTGGGCTTCG TGTATAAACT
     1261 GACGGCAATT AAGCGCAACA ACCGCTGGAT TGGCGTTAAA AAGACGCCGA TCACGGATCT
     1321 GAGCAAGAGC TCCAAGGGTG GTCGTTACAA GACCAAACGC TTGATTACCG TGTACTGAAG
     1381 AGCTTAGAGA CCCTGGAATC AGCCTTGTGT CTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1441 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1501 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1561 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1621 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1681 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1741 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1801 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1861 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1921 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1981 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2041 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2101 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2161 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2221 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2281 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2341 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2401 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2461 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2521 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2581 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2641 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2701 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2761 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2821 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2881 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2941 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3001 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3061 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3121 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3181 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3241 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3301 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3361 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//